Resources

This Resource presents surface and volume atlases of human brain development during early infancy, at monthly intervals.

The AlphaFill algorithm transplants missing small molecules and ions from experimentally determined structures to predicted protein models in the AlphaFold protein structure database. All AlphaFill entries are available for visual inspection and download through the AlphaFill website.

This Resource presents and analyzes four datasets containing both gene expression and morphological profile data for cells subjected to hundreds to thousands of chemical or genetic perturbations and highlights their complementary nature.

This Resource presents a serial block-face EM dataset of the whole larval zebrafish brain, including automated segmentation of neurons, detection of synapses and reconstruction of circuitry for visual motion processing.

This work presents a quantitative draft of the mouse proteome and phosphoproteome constructed from 41 healthy tissues covering 15 major anatomical systems and 66 cell lines.

This resource presents an in silico generated DNA methylation atlas that can be used for cell-type deconvolution of human tissues.

This three-dimensional structural SARS-CoV-2–human interactome web server allows hypothesis-driven exploration of the mechanisms of SARS-CoV-2 pathology and host response.

A knowledgebase developed for increased the transparency of reporting in spheroid research.

CytoSig, an interactive database and model to predict cytokine signaling activity at bulk and single-cell levels.

The LIVECell dataset comprises annotated phase-contrast images of over 1.6 million cells from different cell lines during growth from sparse seeding to confluence for improved training of deep learning-based models of image segmentation.

A protein expression atlas of transcription factors charted onto cell lineage maps of Caenorhabditis elegans development that uncovers mechanisms of spatiotemporal cell fate patterning and regulators of embryogenesis.

GPCRmd is a community-driven online platform to visualize, analyze and share G-protein-coupled receptor (GPCR) molecular dynamics data. It currently contains simulation data representing 100% of GPCR classes, 71% of receptor subtypes and 80% of GPCR families.

The meltome atlas compiles the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans, providing a resource for analyzing protein stability in the context of function and interactions.

This Resource describes genetic tools for microbial eukaryotes, providing a roadmap for developing genetically tractable organisms.

A library of mutant mouse lung endothelial cells expressing a comprehensive repertoire of heparin sulfate structure modifications enables studies of the structure–function relationships of this complex polysaccharide.

A CHO cell library displaying a near-complete repertoire of glycosaminoglycan (GAG) modifications provides a resource for cell-based binding assays, recombinant proteoglycan expression, and assembly of GAG glycan microarrays.

Metagenomic mining generates a rich resource of regulatory sequences with species-selective and universal activity, making it possible to engineer synthetic circuits with tunable gene expression across diverse bacterial hosts.

A collection of 1,406 high-quality, immunoprecipitation- and immunoblotting-grade monoclonal antibodies to 737 human transcription factors is made available as a community resource, along with all validation data.

Based on machine learning-predicted interaction interfaces, this resource enables interpretation of genomic variants and disease mutations in light of the protein-protein interactome.

This work characterizes the maturation kinetics of 50 cyan to far-red fluorescent proteins and provides evidence that proteins that mature faster than their brighter but slower counterparts are more useful for quantitative evaluation of fast processes.