Research Highlights in 2018

A coordinate-based framework quantifies the correlation and interaction of biomolecules in images acquired with super-resolution microscopy techniques.

The multipurpose MAC-tag enables integrative interaction proteomics to localize proteins with sub-organelle precision.

CasRx is a programmable CRISPR–Cas system that targets RNA for efficient knockdown and splicing modulation.

A double-deletion rabies virus variant overcomes the cytotoxicity that has previously limited long-term applications for retrograde labeling of projection neurons.

A deep-learning-based approach improves the speed, accuracy, and robustness of biomedical image reconstruction.

A new approach uses beams of light to direct cytoplasmic flows.

A combination of heat denaturation and mass spectrometry is applied to monitor protein-complex dynamics in the cell.

CRISPR-based imaging of regulatory elements shows that their mobility increases as transcription is initiated.

Upconversion nanoparticles can serve as intermediaries to illuminate optogenetic tools in the mouse brain.

SUSHI is a new method for imaging the extracellular space with high resolution in living brain tissue to reveal insights into the structure and dynamics of the extracellular space.

How to make large synthetic gene libraries out of some beads, an oligo array and an emulsion.

The combination of photoactivatable fluorescent markers with single-cell RNA-seq allows transcriptome analysis of cells from specific tissue locations.

SNP-CLING enables determining the dynamics of alleles on different chromosomes in live cells.

Acoustic reporter genes that produce gas vesicles offer a way to image and locate microorganisms in mammalian hosts.

A method combining limited proteolysis with mass spectrometry systematically detects protein–metabolite interactions.

The combination of DNA-PAINT and spinning-disk confocal microscopy makes super-resolution microscopy in whole cells affordable and easy to implement.

A computational framework enables automated annotation, analysis, and sharing of mouse brain data at single-cell resolution.

Methylation patterns can help determine the origin of metagenomic sequences.

Cellular engineering that allows budding yeast to survive with the four core human histones opens the door to exploring the function of histone variants and their modifications.

Virus stamping can target single cells in complex tissues, both in culture and in vivo.