News & Views

  • News & Views |

    Understanding of fundamental questions in human embryology is hampered by limited access to in utero developmental events. Manfrin et al. have engineered an in vitro platform that recapitulates early morphogenic events of human development with unprecedented spatial and temporal control.

    • Leonardo Morsut
    •  & Giorgia Quadrato
  • News & Views |

    The interpretation of fragmentation patterns in tandem mass spectrometry is crucial for peptide sequencing, but the relative intensities of these patterns are difficult to predict computationally. Two groups have applied deep neural networks to address this long-standing problem in the proteomics field, extending theoretical spectra with an additional dimension of high-accuracy fragment ion intensities.

    • Hannes L. Röst
  • News & Views |

    Light-sheet fluorescence microscopes are making it possible to follow subcellular dynamics with unprecedented detail, but they are complex to build and maintain. A new method that can generate arbitrary light sheets provides much-needed simplicity and additional versatility.

    • Manu Forero-Shelton
  • News & Views |

    A recent approach for single-cell RNA-sequencing data uses Bayesian deep learning to correct technical artifacts and enable accurate and multifaceted downstream analyses.

    • Gregory P. Way
    •  & Casey S. Greene
  • News & Views |

    A type of neural network first described in 2015 can be trained to translate between images of the same field of view acquired by different modalities. Trained networks can use information inherent in grayscale images of cells to predict fluorescent signals.

    • Roger Brent
    •  & Laura Boucheron
  • News & Views |

    A new article by Pandarinath et al. describes an artificial neural network model that captures some key aspects of the activity of populations of neurons in the primary motor cortex.

    • Aaron P. Batista
    •  & James J. DiCarlo
  • News & Views |

    A new detector built for X-ray free-electron lasers provides unprecedented speed and accuracy for macromolecular crystallography at synchrotron radiation facilities—and finally allows crystallographers to harness the full capabilities of those sources.

    • Henry N. Chapman
  • News & Views |

    Overexpression of mouse thymic-stromal-cell-derived lymphopoietin in immune-compromised mice that harbor a reconstituted human immune system rescues lymph node formation and enhances adaptive immune responses.

    • Alexandre P. A. Theocharides
    •  & Markus G. Manz
  • News & Views |

    Quanti.us is a platform that allows large-scale manual image annotation by a distributed workforce through Amazon’s Mechanical Turk crowdsourcing marketplace.

    • Martin L. Jones
    •  & Helen Spiers
  • News & Views |

    Two methods for controlling protein activities with small molecules provide a general solution to a long-standing challenge in mammalian synthetic biology.

    • Bryan C. Dickinson
  • News & Views |

    An interpretable, deep neural network produces mechanistic hypotheses on how genetic interactions contribute to whole-cell phenotypes.

    • Kristin Branson
  • News & Views |

    Two complementary approaches improve 3D mass spectrometry imaging of biomolecules on 3D surfaces and within tissue sections and single cells.

    • Klaus Dreisewerd
    •  & Joanne Y Yew
  • News & Views |

    Direct RNA quantification using barcoded fluorescence hybridization enables accurate and scalable gene expression profiling.

    • Jinzhou Yuan
    •  & Peter A Sims
  • News & Views |

    Advances in image scanning microscopy move super-resolution imaging deeper into tissues with faster visualization and finer details.

    • Sam Duwé
    •  & Peter Dedecker
  • News & Views |

    High-density multielectrode arrays in turtle cortex allow parallel assessment of functional and structural connectomics.

    • Harvey A Swadlow
    •  & Jose-Manuel Alonso
  • News & Views |

    Useful new methods are being introduced to experimentally determine the genome-wide consequences of Cas9-based editing.

    • Fyodor D Urnov
  • News & Views |

    Two complementary approaches for directing human hematopoietic stem cells along the T cell lineage will have applications in both fundamental and translational research.

    • Anne-Catherine Dolens
    •  & Tom Taghon
  • News & Views |

    Advanced signal processing techniques enable the genome-wide analysis of methylation in native DNA using nanopore sequencing.

    • Michael C Schatz
  • News & Views |

    Four studies overcome the limitations of pooled and arrayed genetic screens by integrating single-cell transcriptomics.

    • Daniel E Wagner
    •  & Allon M Klein
  • News & Views |

    A sequencing approach, m6A-LAIC-seq, globally quantifies the proportion of m6A-modified transcripts of a specific gene and the differential m6A modification levels among its isoforms.

    • Hailing Shi
    •  & Chuan He
  • News & Views |

    A new statistical approach mitigates technical errors in single-cell DNA sequencing data to advance the study of tumor evolution and diversity.

    • Nicholas E Navin
    •  & Ken Chen
  • News & Views |

    Two new approaches for target profiling of RNA-binding proteins require less cell material, avoid radioactive reagents and give more accurate data in a shorter time compared to previous methods.

    • Georges Martin
    •  & Mihaela Zavolan
  • News & Views |

    Lattice light-sheet-PAINT brings localization microscopy to large volumes, and SuReSim enables ground truth modeling of super-resolution experiments.

    • Ralf Jungmann
  • News & Views |

    To drive its migration through a fibrillar matrix—and thus to spread, invade or metastasize—a cancer cell must exert physical forces. The first visualization of these forces in three dimensions reveals surprising migration dynamics.

    • Karin Wang
    • , Li-Heng Cai
    • , Bo Lan
    •  & Jeffrey J Fredberg
  • News & Views |

    Thermal profiling in combination with quantitative proteomics makes it possible to identify cellular membrane protein targets of small molecules.

    • Yves Leestemaker
    •  & Huib Ovaa
  • News & Views |

    Two approaches for robust, objective segmentation of single-molecule localization data promise better quantitative insights into protein clustering from super-resolution imaging methods.

    • David Baddeley
  • News & Views |

    An integrated single-molecule fluorescence approach enables the study of nano-to-millisecond protein conformational dynamics in living mammalian cells.

    • Anne Plochowietz
    •  & Achillefs N Kapanidis
  • News & Views |

    Nanopore sequencing gets a boost with accurate error modeling and variant-calling tools for Oxford Nanopore Technology's highly anticipated MinION platform.

    • Nicholas J Loman
    •  & Mick Watson