Method to Watch

Mechanoimmunology methods help dissect the mechanical forces within the immune system.

CRISPR systems can be leveraged to direct recombinases to integrate gene-sized DNA sequences.

Strategies to overcome poor reproducibility in MRI studies are needed.

In vitro models and single-cell atlases help uncover the mysteries of human embryogenesis.

Computational approaches are pushing the limits of unknown metabolite annotation.

Advances in fluorescence microscopy and spectroscopy show their promise for applications that complement in situ structural biology methods like cryoelectron tomography.

The combination of microscopy, targeted illumination and single-cell sequencing is driving applications from direct evolution to spatial omics.

Data integration methods weave genomics data into holistic pictures of biological systems.

Lineage tracing methods help unravel complexities of cell state and differentiation programs.

Viral tools are a mainstay in mammalian neuroscience, but there is room for improvement.

Advances in label-free microscopy are expanding experimental observations, from biophysics to cell biology and beyond.

The diverse microbial world provides unprecedented potential for exploring genome editing tools.

Method development is indispensable for obtaining complete genomes, and deciphering them.

High-throughput and ultrasensitive mass spectrometry–based approaches are gaining ground in subcellular compositional analysis.

Probing spatially organized DNA and its interacting elements in single cells will deepen our understanding of cell-type-specific gene regulation.

Emerging algorithms are extracting information about macromolecular motions from cryo-EM data.

Glycoproteomics is coming of age, thanks to advances in instrumentation, experimental methodologies and computational search algorithms.

Organoids generated by spatially organizing multiple cell types, called assembloids, will enable deeper insights into tissue function.

Computational approaches help us explore complexities of the T cell receptor repertoire.

Method development pushes the limit of completeness of genome sequences.