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Compared in this Analysis are two widely used procedures for ribosomal RNA removal in metatranscriptomic samples, and the authors present recommendations to prevent misleading analyses of microbial communities.
A comparison of ordination analysis methods used to reveal gradients or clusters in sequence data from microbial communities reveals which methods are best suited for which community structure at different sequencing depth.
The authors compare quality metrics of libraries from seven strand-specific RNA-seq methods in terms of complexity, strand specificity, evenness and continuity of coverage, and expression profiling. They provide a computational pipeline to compare these metrics from any RNA-seq protocol.