Research Highlights

As more phage-derived Cas9 inhibitors are discovered, their detailed characterization is a prerequisite to using them as research tools.

A recombinase-based barcoding approach enables fate mapping in the hematopoietic system under physiological conditions.

DNA and chromatin structures can be visualized in situ with electron tomography.

Through scattering, in situ metal particle growth enables biomolecule visualization within tissue.

An intermediate cell state of chemical reprogramming can be induced to convert directly between cell types.

A self-inactivating rabies virus enables long-term tracing of neural networks without toxicity.

A large-scale approach to analyze how protein sequence determines folding provides new insights into an old question.

Spontaneously blinking membrane probes enable live-cell super-resolution imaging for up to half an hour.

CRISPR-mediated chromatin looping provides insight into the effect of genomic structure on gene expression.

Developments in data-independent-acquisition mass spectrometry allow efficient and accurate quantitation of peptidoforms across large sample cohorts.

A chemical caging strategy allows mCherry to be used for localization microscopy.

Combining an inducible methyltransferase with a methylation-sensitive zinc finger creates an epigenetic memory circuit.

The Metafluidics initiative encourages researchers to upload and share open-source microfluidics designs.

A tapered optical fiber delivers spatially precise and efficient optogenetic illumination to the rodent brain.

In vitro Cas9-binding assays show the effects of mutations in the target sequence on binding kinetics.

Single-cell transcriptomics is used to determine what cell types are present in brain organoids and how much these cell types vary across organoids.

A serial-section electron microscopy data set of larval zebrafish brain—imaged at several scales—provides a resource for structure–function analyses of the animals' neural circuitry.

Human protein subcellular locations light up in the Cell Atlas resource.

Two sets of complementary tools extend our capacity to manipulate the gut microbiome.

A multicolor reporter mouse model enables monitoring of the fate and dynamics of microglia in health and disease.