Research Highlights

Two novel techniques refine genome-wide mapping of R loops.

Building redundancy into fluorogenic sequencing makes for error-free DNA sequence reads.

Using antibodies to target specific proteins for rapid degradation is a versatile approach for studying the function of endogenous proteins.

Reflective coverslips can improve spatiotemporal resolution and collection efficiency in diSPIM light-sheet fluorescence microscopy.

Trans-Tango allows trans-synaptic mapping of presynaptic and postsynaptic partners in Drosophila.

Combining red calcium indicators with an optimized illumination strategy enables neuronal activity to be imaged in deep layers of the mouse cortex and hippocampus.

Ultradeep sequencing of Hi-C data from mouse cells in different developmental stages can address outstanding questions about 3D chromatin function.

A class of semiconductor polymer particles can be used for afterglow imaging to avoid autofluorescence when imaging deep structures in living animals.

Protein evolution yields a programmable base editor that can revert the most common pathogenic point mutations in the genome.

A technique combining mass spectrometry and infrared spectroscopy shows promise for unambiguous sequencing of linear oligosaccharides.

Ultralow concentrations of DNA can be optically sequenced with SMRT DNA sequencing.

An optical setup for observing targeted fluorophores lasing from within tissue.

A method to analyze intact glycopeptides on a proteome-wide scale almost doubles the number of confirmed glycoproteins.

Two new methods simultaneously measure epitope and transcriptome levels in single cells.

Image-guided targeting of fluorescently labeled neurons enables automated electrophysiological recordings of defined neuronal cell types.

Antibodies that initiate signaling cascades and thereby replace reprogramming factors promise an efficient and safe way to produce pluripotent stem cells.

Fate maps of human brain cancer cells, transplanted in mouse, challenge traditional views of tumor genomic heterogeneity.

An integrated workflow allows the quantitative interrogation of the impact of alternative splicing at the proteome level.

A method to detect highly reactive lysine residues in the proteome helps spot those ripe for targeting by covalent ligands.

Single-particle reconstruction algorithms can be used to obtain 3D structures from 2D localization microscopy images.