Research Highlights

Independent efforts shine light on the 3D genome structure by looking at multiple contacts along an allele or equalizing the distance between restriction sites for higher-resolution Hi-C maps.

The genetically encoded sensor dLight1 reports dopamine release in culture, slices and behaving mice.

Cas9 induces larger-than-anticipated mutations in mouse and human cells. In the latter, efficient editing depends on inhibition of the DNA-damage-repair protein p53.

Reversible primer termination, enabled by polymerase–nucleotide conjugates, provides an enzymatic method for the de novo synthesis of oligonucleotides.

Self-assembled nanoparticles give insight into the regulation of macromolecular crowding.

The STARmap method advances RNA sequencing in intact tissues.

Two new methods provide high-resolution profiles of splicing events in yeast.

Aberration correction adds depth to light-sheet microscopy.

3C-based methods and polymer modeling indentify the impact of structural variants.

A combination of behavioral analysis and neural activity recording provides a glimpse into how the brain orchestrates complex behaviors.

Interferometric scattering microscopy enables quantitative mass imaging and biophysical characterization of single biomolecules in solution.

Inside a mouse brain, human cerebral organoids can show their potential.

Chromatin-associated RNA sequencing elucidates the role of RNAs in genome regulation.

Biobeam is a software package that simulates optical effects during imaging within scattering tissue, which could help improve image acquisition and extraction.

PDB-Dev hosts integrative structural models of biomolecular assemblies solved by hybrid methods.

Two approaches apply deep learning to improve single-molecule localization microscopy.