News & Views

  • News & Views |

    Robots can be used to detect marked animals with less disturbance when assessing ecological drivers of population change.

    • Philip N Trathan
    •  & Louise Emmerson
  • News & Views |

    Use of adenoviral vectors to deliver donor templates for genome editing facilitates precise genome modifications in human cells. This has implications for both basic and translational applications of rare-cleaving nuclease technologies.

    • Andrew Scharenberg
  • News & Views |

    A new distributed computing framework for data analysis enables neuroscientists to meet the computational demands of modern experimental technologies.

    • John P Cunningham
  • News & Views |

    Barcoded semisynthetic nucleosomes combined with massively parallel sequencing provide an innovative new platform for analyzing the histone-recognition and histone-modifying activities of chromatin-associated proteins.

    • Erin K Shanle
    • , Scott B Rothbart
    •  & Brian D Strahl
  • News & Views |

    Experimenter gender affects behavioral assays of pain and stress in laboratory mice.

    • Melissa Bateson
  • News & Views |

    The combination of mass spectroscopy with immunohistochemistry allows highly multiplexed, directly quantitative imaging of tissue samples for both basic and clinical research.

    • David L Rimm
  • News & Views |

    A first community experiment comparing the performance of analysis methods for single-particle tracking data declares no winner but reveals valuable information for users and developers.

    • Michael J Saxton
  • News & Views |

    An approach is described that simplifies the isolation of rare human pluripotent stem cells engineered to carry precise disease-relevant mutations.

    • Ryan Forster
    •  & Dirk Hockemeyer
  • News & Views |

    A new family of genetically encoded ratiometric calcium indicators optimized for imaging of calcium signals in vivo exhibits near-linear fluorescence dynamics while minimizing artifacts caused by movement.

    • Christian D Wilms
    •  & Michael Häusser
  • News & Views |

    New methods for measuring the sensitivity of chromatin to DNase digestion and Tn5 transposition help us map and interpret the genome's regulatory sequences.

    • Anil Raj
    •  & Graham McVicker
  • News & Views |

    RNA-seq is a recent and immensely popular technology for cataloging and comparing gene expression. Two papers from the international RGASP consortium report on large-scale competitions to identify the best algorithms for RNA-seq analysis, with surprising variability in the results.

    • Ian Korf
  • News & Views |

    Use of helper interactions to encourage weak heteromeric interactions between fluorescent protein pairs helps ensure optimal fluorescence resonance energy transfer (FRET) signals and minimizes the impact on target protein interactions.

    • Kees Jalink
  • News & Views |

    An algorithm called CONTACT identifies correlated side-chain motions in proteins from X-ray crystallographic data, providing insights into dynamics and function.

    • Mark A. Wilson
  • News & Views |

    Massive parallelization of scanning-based super-resolution imaging allows fast imaging of large fields of view.

    • Christopher J Rowlands
    • , Elijah Y S Yew
    •  & Peter T C So
  • News & Views |

    Methods for high-throughput and high-resolution dissection of enhancers in Drosophila are described by two independent groups.

    • Matthew Slattery
    •  & Kevin P White
  • News & Views |

    More than 40 years after the invention of semiconductor pH microtransducers known as ISFETs, this transistor-based technology may revolutionize quantitative PCR.

    • Carlotta Guiducci
    •  & Fabio M Spiga
  • News & Views |

    Two approaches to serially enrich protein post-translational modifications allow the detection of multiple modifications in a single biological sample using mass spectrometry.

    • Kristofor Webb
    •  & Eric J Bennett
  • News & Views |

    Two reports describing the use of direct-conversion electron detectors and algorithms that correct for beam-induced sample motion in single-particle electron cryomicroscopy demonstrate that this technique can solve structures of macromolecules at near-atomic resolution.

    • Robert M. Glaeser
  • News & Views |

    Classic gene targeting and gene replacement can now be achieved in zebrafish after cleaving the genome with engineered nucleases in the presence of donor DNA. This simple-to-implement method enables new classes of biological study in this important model organism.

    • David Jonah Grunwald
  • News & Views |

    Statistical analysis of single-molecule tracking data maps the number of states occupied by a molecule in a bacterial cell.

    • Ji Yu
  • News & Views |

    Classification of proteins by ligand binding similarity offers an alternative approach to evolutionary methods for organizing and understanding biology, allowing new insights into protein function and physiological signal transduction.

    • Gerard J P van Westen
    •  & John P Overington
  • News & Views |

    A fully automated pipeline that systematically models three-dimensional (3D) structural details of protein interactions will allow researchers to interpret perturbation effects within protein pathways and networks.

    • Joan Teyra
    •  & Philip M Kim
  • News & Views |

    The Genome Multitool (GEM) mapper rapidly and accurately provides all alignments of a read within a user-defined number of mismatches.

    • Gregory G Faust
    •  & Ira M Hall
  • News & Views |

    A new toolbox for structural biology that combines single-molecule fluorescence and molecular modeling is used to generate high-precision structures of protein complexes.

    • Timothy D Craggs
    •  & Achillefs N Kapanidis
  • News & Views |

    Researchers adapt a popular Orbitrap-based mass spectrometer to detect and analyze large, intact protein complexes, accelerating a new frontier in structural biology.

    • Philip D Compton
    •  & Neil L Kelleher
  • News & Views |

    A new high-throughput method for monitoring G protein–coupled receptor activation is highly suited to assaying Gα12/13-coupled receptors and is used to deorphanize a group of receptors activated by lysophosphatidylserine.

    • Marc Parmentier
  • News & Views |

    Two studies plant a signpost on the road toward a robust and detailed chromatin interaction map.

    • Xianghong Jasmine Zhou
    •  & Frank Alber
  • News & Views |

    Through a collaborative effort, engineers and neuroscientists have created a method, using electrospun nanofibers as surrogate neuronal axons, to piece together the complexities of myelination.

    • Ragnhildur Thóra Káradóttir
    •  & John Henry Stockley
  • News & Views |

    An experimental infrastructure consisting of environmentally controlled and spatially linked habitat patches permits studies on terrestrial animal dispersal at an unprecedented scale for an experiment with such strict control.

    • Nick M Haddad
  • News & Views |

    In toto imaging of living embryos has now become much faster. Light-sheet illumination and fluorescence detection with four objective lenses provide complete coverage of large samples in a snap.

    • Michael Weber
    •  & Jan Huisken
  • News & Views |

    A precomputed database of lineage-restricted reference genes yields a fast and accurate tool that uses sequence similarity alone to compute clade abundances from shotgun metagenomic data sets.

    • Daniel H Haft
    •  & Andrey Tovchigrechko