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Although structural variation is less explored than single-nucleotide variation, recent studies have shown it to be associated with several human diseases. Three fresh computational methods might help to elucidate this inadequately understood part of our genetic makeup.

Cue achieves versatile and performant structural variant calling and genotyping using a deep-learning approach.

Two miniature microscopes with innovative light paths are described and applied to imaging of juvenile zebra finches and mice.

Femtosecond laser microdissection enables transcriptomic analyses of single neurons based on their phenotype.

Outbreak.info empowers real-time variant monitoring and tracing of associated publications and resources during the ‘infodemic’ of SARS-CoV-2.

This Review describes advances in cryogenic electron tomography on focused ion beam lamellae, highlighting the key benefits of this technology for in situ structural biology and discussing important future directions.

The nanopore community is stepping toward a new frontier of single-molecule protein sequencing. Here, we offer our opinions on the unique potential for this emerging technology, with a focus on single-cell proteomics, and some challenges that must be overcome to realize it.

The development of mass spectrometry-based single-cell proteomics technologies opens unique opportunities to understand the functional crosstalk between cells that drive tumor development.

It’s a creative, perhaps wild and sometimes challenging encounter. But some labs make a habit of inviting artists in.

A bioengineering and machine learning-guided approach models human axial development and somitogenesis in vitro.

Single-cell proteomics is a challenging goal and an area of rapid methods development. This Focus issue highlights the many paths toward high-throughput, high-sensitivity measurements.

Recent technological advances in mass spectrometry promise to add single-cell proteomics to the biologist’s toolbox. Here we discuss the current status and what is needed for this exciting technology to lead to biological insight — alone or as a complement to other omics technologies.

Increasing evidence suggests that the spatial distribution of biomolecules within cells is a critical component in deciphering single-cell molecular heterogeneity. State-of-the-art single-cell MS imaging is uniquely capable of localizing biomolecules within cells, providing a dimension of information beyond what is currently available through in-depth omics investigations.

New instruments are emerging in single-cell and single-molecule proteomics. This will offer labs a choice of technologies.

We argue that the study of single-cell subcellular organelle omics is needed to understand and regulate cell function. This requires and is being enabled by new technology development.