Genome assembly projects get a boost from high-accuracy long-read sequencing.

This Review describes proximity labeling methods that make use of peroxidases (APEX) or biotin ligases (TurboID, BioID), and their applications to studying protein–protein and protein–nucleic acid interactions in living systems.

The Perspective reviews the computational methods for subclonal reconstruction and recommends best practices for analyses and quality assessments.

Advances in single-cell sequencing technologies enable generation of datasets of millions of cells. scfind facilitates efficient and sophisticated gene search in massive single-cell datasets.

HD-fMOST is a microscopy technique for imaging large samples at high throughput and with high definition, which is achieved with a line-illumination modulation approach. The technology is illustrated by imaging fluorescently labeled neurons in whole mouse brains.

This work reports a dual transposase–peroxidase fusion to survey the accessible chromatin regions and the proximal proteome in one assay, providing a tool to capture both the genomic and proteomic contents of open chromatin.

Commonly used organic dyes can photoconvert to blue-shifted fluorescent species, especially under intense illumination. The mechanism of this process and how to avoid unwanted artifacts in super-resolution microscopy are explored here.

Connecting the Ming dynasty to sharper views in brain imaging.

A genetically encoded neurotransmitter sensor, engineered from a tick lipocalin, detects serotonin in the living brain.

Beyond the well-known pantheon of model organisms are others. A shift is underway to level the playing field.

Global protein structure readouts help detect protein functional changes.

DeepLabCut-Live! performs pose estimation in real time and allows closed-loop feedback on behavior.

Loop-seq is a high-throughput sequencing assay that measures DNA looping and can help explain how DNA bendability contributes to nucleosome organization.