Flexible and fatigue-resistant optical fibers made from hydrogel allow optogenetic manipulations in the periphery in freely behaving mice.

This Perspective introduces advances in quantitative phase imaging and artificial intelligence-based image analysis and further describes how the two technologies intersect and synergize to enable biomedical research.

This analysis leverages experimentally sequenced data and in silico mixtures to simulate transcript expression differences, which enables a performance assessment of long-read tools developed for isoform detection, differential transcript expression analysis and differential transcript usage analysis.

Ultrasound-based modalities are revealing the brain’s inner workings with steadily increasing speed, resolution and depth.

Next-generation red and green G-protein-coupled receptor-based dopamine sensors with improved properties have been developed. Their performance is demonstrated in cell culture, in brain slices and in vivo in the mouse.

An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of predicted structures do not agree with experimentally resolved data. Therefore, care must be taken when using these predictions for informing structural hypotheses.

An integrative framework to simultaneously interrogate the dynamics of the transcriptome and proteome at subcellular resolution that combines two methods, localization of RNA (LoRNA) and a streamlined density-based localization of proteins by isotope tagging (dLOPIT).

Monomeric and tandem dimer derivatives of the bright and photostable green fluorescent protein StayGold offer versatile tools for tagging target proteins and membranes in extended live-cell imaging.

By combining fast lift-over and selective re-mapping, levioSAM2 enables efficient and accurate read mapping and variant calling leveraging complete reference genomes.

A combination of hardware developments has increased the achievable spatial resolution in 7 Tesla human neuroimaging to about 0.4 mm.

Labs range in size, and no one size is likely to fit all. Here some researchers share their lab size experiences and preferences.

This month we continue our annual celebration of beautiful images.

Newly developed DNA devices can be used to study intracellular sodium and potassium ion levels with organellar resolution.