All life sciences research is potentially subject to ethical considerations. Institutions should support collaborations with professional ethicists and philosophers to help life scientists navigate ethical crossroads.

The global imaging community is pursuing innovative approaches to achieve more equitable access to instruments and expertise.

The capability of high-resolution in situ imaging by electron cryo-tomography (cryo-ET) has now been expanded to large multicellular tissues by newly developed workflows involving lift-out and serial sectioning using focused ion beam milling under cryogenic conditions.

This Perspective discusses the integration of small-scale datasets with each other or with larger reference atlases, particularly in the context of single-cell approaches.

Serialized on-grid lift-in sectioning for tomography (SOLIST) improves the throughput of the serial lift-out technique for creating lamellas, addressing a major bottleneck in the use of cryo-electron tomography for in situ structural biology.

FICTURE is a segmentation-free approach for identifying tissue architecture in spatial transcriptomics data. FICTURE is compatible with both imaging-based and sequencing-based methods and is uniquely suited for handling the largest available datasets.

SN2N, a Self-inspired Noise2Noise module, offers a versatile solution for volumetric time-lapse super-resolution imaging of live cells. SN2N uses self-supervised data generation and self-constrained learning for training with a single noisy frame.

This work introduces a k-mer-based approach to customizing a pangenome reference, making it more relevant to a new sample of interest. This method enhances the accuracy of genotyping small variants and large structural variants.

Enhanced Classification of Localized Point clouds by Shape Extraction (ECLiPSE) is a robust feature extraction and classification pipeline for diverse and heterogeneous structures in both 2D and 3D single-molecule localization microscopy data.

MiLoPYP is a two-step, dataset-specific contrastive learning-based method for fast and accurate detection and localization of a diverse range of target structures in cryo-electron tomography data, enabling improved in situ structural biology.

All life sciences research is potentially subject to ethical considerations. Institutions should support collaborations with professional ethicists and philosophers to help life scientists navigate ethical crossroads.