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News & Comment

  • News & Views |

    Single objective light-sheet fluorescence microscopes combine the convenience of conventional sample mounting with sensitive subcellular and super-resolution imaging of cells and tissues.

    • Reto Fiolka
  • Technology Feature |

    As single-cell proteomics emerges, perhaps labs can avoid the need to infer protein levels from mRNA abundances.

    • Vivien Marx
  • This Month |

    Staying mobile and building a Toy-Story-related way to stitch terabyte-sized images in microscopy.

    • Vivien Marx
  • Comment |

    Whole-body energy expenditure is the summed metabolic activities of tissues and, to remove the influence of body size, ratios of energy expenditure to body mass are often applied but can generate spurious differences. In 2011, a group of experts proposed adoption of ANCOVA for the analysis of metabolic rate but, seven years later, analyses based on ratios remain the most frequent. We discuss some of the barriers to adopting better analytical procedures.

    • Rodrigo Fernández-Verdejo
    • , Eric Ravussin
    • , John R. Speakman
    •  & Jose E. Galgani
  • Comment |

    The PLUMED consortium unifies developers and contributors to PLUMED, an open-source library for enhanced-sampling, free-energy calculations and the analysis of molecular dynamics simulations. Here, we outline our efforts to promote transparency and reproducibility by disseminating protocols for enhanced-sampling molecular simulations.

    • Massimiliano Bonomi
    • , Giovanni Bussi
    • , Carlo Camilloni
    • , Gareth A. Tribello
    • , Pavel Banáš
    • , Alessandro Barducci
    • , Mattia Bernetti
    • , Peter G. Bolhuis
    • , Sandro Bottaro
    • , Davide Branduardi
    • , Riccardo Capelli
    • , Paolo Carloni
    • , Michele Ceriotti
    • , Andrea Cesari
    • , Haochuan Chen
    • , Wei Chen
    • , Francesco Colizzi
    • , Sandip De
    • , Marco De La Pierre
    • , Davide Donadio
    • , Viktor Drobot
    • , Bernd Ensing
    • , Andrew L. Ferguson
    • , Marta Filizola
    • , James S. Fraser
    • , Haohao Fu
    • , Piero Gasparotto
    • , Francesco Luigi Gervasio
    • , Federico Giberti
    • , Alejandro Gil-Ley
    • , Toni Giorgino
    • , Gabriella T. Heller
    • , Glen M. Hocky
    • , Marcella Iannuzzi
    • , Michele Invernizzi
    • , Kim E. Jelfs
    • , Alexander Jussupow
    • , Evgeny Kirilin
    • , Alessandro Laio
    • , Vittorio Limongelli
    • , Kresten Lindorff-Larsen
    • , Thomas Löhr
    • , Fabrizio Marinelli
    • , Layla Martin-Samos
    • , Matteo Masetti
    • , Ralf Meyer
    • , Angelos Michaelides
    • , Carla Molteni
    • , Tetsuya Morishita
    • , Marco Nava
    • , Cristina Paissoni
    • , Elena Papaleo
    • , Michele Parrinello
    • , Jim Pfaendtner
    • , Pablo Piaggi
    • , GiovanniMaria Piccini
    • , Adriana Pietropaolo
    • , Fabio Pietrucci
    • , Silvio Pipolo
    • , Davide Provasi
    • , David Quigley
    • , Paolo Raiteri
    • , Stefano Raniolo
    • , Jakub Rydzewski
    • , Matteo Salvalaglio
    • , Gabriele Cesare Sosso
    • , Vojtěch Spiwok
    • , Jiří Šponer
    • , David W. H. Swenson
    • , Pratyush Tiwary
    • , Omar Valsson
    • , Michele Vendruscolo
    • , Gregory A. Voth
    •  & Andrew White

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

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