All life sciences research is potentially subject to ethical considerations. Institutions should support collaborations with professional ethicists and philosophers to help life scientists navigate ethical crossroads.

The global imaging community is pursuing innovative approaches to achieve more equitable access to instruments and expertise.

The capability of high-resolution in situ imaging by electron cryo-tomography (cryo-ET) has now been expanded to large multicellular tissues by newly developed workflows involving lift-out and serial sectioning using focused ion beam milling under cryogenic conditions.

This Perspective discusses the integration of small-scale datasets with each other or with larger reference atlases, particularly in the context of single-cell approaches.

Genes2Genes is a dynamic programming framework that enables precise alignment for single-cell trajectories at the per-gene level.

Cortical development is influenced by morphogen gradients. To mimic patterning events during brain development, polarized cortical assembloids are generated with the help of a localized FGF8 source.

CAST is a deep learning-based method that enables across-sample searching and matching based on spatial molecular features and reconstructing spatially resolved single-cell multi-omic profiles, as well as supports downstream differential analysis.

The Genomics 2 Proteins portal is an open-source tool for proteome-wide linking of human genetic variants to protein sequences and structures. The portal serves as a discovery tool to hypothesize the structure–function relationship between natural or synthetic variations and their molecular phenotypes.

Arkitekt is an open-source platform that facilitates the implementation of complex quantitative bioimaging workflows in real time, from acquisition to visualization and analysis.

Serialized on-grid lift-in sectioning for tomography (SOLIST) improves the throughput of the serial lift-out technique for creating lamellas, addressing a major bottleneck in the use of cryo-electron tomography for in situ structural biology.

Developing new methods takes passion and a penchant for risk-taking.

I don’t have good luck in the match points. —Rafael Nadal, Spanish tennis player.

All life sciences research is potentially subject to ethical considerations. Institutions should support collaborations with professional ethicists and philosophers to help life scientists navigate ethical crossroads.