All life sciences research is potentially subject to ethical considerations. Institutions should support collaborations with professional ethicists and philosophers to help life scientists navigate ethical crossroads.

The global imaging community is pursuing innovative approaches to achieve more equitable access to instruments and expertise.

The capability of high-resolution in situ imaging by electron cryo-tomography (cryo-ET) has now been expanded to large multicellular tissues by newly developed workflows involving lift-out and serial sectioning using focused ion beam milling under cryogenic conditions.

This Perspective discusses the integration of small-scale datasets with each other or with larger reference atlases, particularly in the context of single-cell approaches.

20ExM achieves isotropic ~20× expansion of cells and tissues in a single shot for super-resolution imaging with <20-nm resolution on a conventional microscope.

SCUBA-D is a denoising diffusion probabilistic model that can perform a variety of protein backbone design tasks, including generation of backbones from random noise, from user-sketched designs, and backbones to scaffold predefined motifs that can bind small molecules or other proteins.

Confocal light-field microscopy allows volumetric voltage imaging in the mouse brain at speeds sufficiently high to extract the spiking activity of hundreds of neurons.

Combining localization and polarization microscopy can yield detailed insights into subcellular structures. POLCAM uses a polarization camera and wide-field microscopy for rapid measurement of super-resolution orientation imaging in live cells.

To avoid head fixation or drawbacks of miniaturized devices for freely moving rodents, a robotic device can move a headstage for microscopy or electrophysiology with the animal, thereby enabling naturalistic behavior.

This work presents scNanoSeq-CUT&Tag, using long-read sequencing to profile histone modifications and DNA-binding proteins at the single-cell level.

For Nature Methods’ 20th anniversary, our current and past editors reminisce about their favorite papers, initiatives and projects at the journal.

For Nature Methods’ 20th anniversary, our current and past editors reminisce about their favorite papers, initiatives and projects at the journal.

For Nature Methods’ 20th anniversary, our current and past editors reminisce about their favorite papers, initiatives and projects at the journal.

For Nature Methods’ 20th anniversary, our current and past editors reminisce about their favorite papers, initiatives and projects at the journal.