Method of the Year 2020: spatially resolved transcriptomics

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Latest Research

  • Review Article |

    This Review describes proximity labeling methods that make use of peroxidases (APEX) or biotin ligases (TurboID, BioID), and their applications to studying protein–protein and protein–nucleic acid interactions in living systems.

    • Wei Qin
    • , Kelvin F. Cho
    • , Peter E. Cavanagh
    •  & Alice Y. Ting
  • Article |

    EXODUS is a high-speed isolation method for the enrichment of exosome from biological fluids with high purity and yield.

    • Yuchao Chen
    • , Qingfu Zhu
    • , Liming Cheng
    • , Yong Wang
    • , Meng Li
    • , Qinsi Yang
    • , Liang Hu
    • , Doudou Lou
    • , Jiaoyuan Li
    • , Xianjun Dong
    • , Luke P. Lee
    •  & Fei Liu
  • Brief Communication |

    A two-photon miniature microscope with enlarged field of view and axial scanning capabilities has been developed and applied in freely moving mice.

    • Weijian Zong
    • , Runlong Wu
    • , Shiyuan Chen
    • , Junjie Wu
    • , Hanbin Wang
    • , Zhe Zhao
    • , Guoqing Chen
    • , Rui Tu
    • , Danlei Wu
    • , Yanhui Hu
    • , Yangyang Xu
    • , Yao Wang
    • , Zhuoli Duan
    • , Haitao Wu
    • , Yunfeng Zhang
    • , Jue Zhang
    • , Aimin Wang
    • , Liangyi Chen
    •  & Heping Cheng
  • Article |

    Megabodies, built by grafting nanobodies onto larger protein scaffolds, help alleviate problems of particle size and preferential orientation at the water–air interfaces during cryo-EM based structure determination experiments and are shown to be generalizable to soluble and membrane-bound proteins.

    • Tomasz Uchański
    • , Simonas Masiulis
    • , Baptiste Fischer
    • , Valentina Kalichuk
    • , Uriel López-Sánchez
    • , Eleftherios Zarkadas
    • , Miriam Weckener
    • , Andrija Sente
    • , Philip Ward
    • , Alexandre Wohlkönig
    • , Thomas Zögg
    • , Han Remaut
    • , James H. Naismith
    • , Hugues Nury
    • , Wim Vranken
    • , A. Radu Aricescu
    • , Els Pardon
    •  & Jan Steyaert

News & Comment

  • Method to Watch |

    Glycoproteomics is coming of age, thanks to advances in instrumentation, experimental methodologies and computational search algorithms.

    • Arunima Singh
  • Method to Watch |

    Organoids generated by spatially organizing multiple cell types, called assembloids, will enable deeper insights into tissue function.

    • Nina Vogt
  • Method to Watch |

    Computational approaches help us explore complexities of the T cell receptor repertoire.

    • Madhura Mukhopadhyay
  • Method to Watch |

    Method development pushes the limit of completeness of genome sequences.

    • Lin Tang
  • Method to Watch |

    Prime editors enable different types of small mutations to be introduced into genomes.

    • Lei Tang

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

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