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Latest Research

  • Article |

    The DNA-based, ratiometric fluorescent reporter CalipHluor enables quantitative imaging of pH and calcium in acidic organelles with single-organelle resolution. The probe was used to identify a lysosome-specific Ca2+ importer in animals.

    • Nagarjun Narayanaswamy
    • , Kasturi Chakraborty
    • , Anand Saminathan
    • , Elizabeth Zeichner
    • , KaHo Leung
    • , John Devany
    •  & Yamuna Krishnan
  • Article |

    fMRIPrep is a robust and easy-to-use pipeline for preprocessing of diverse fMRI data. The transparent workflow dispenses of manual intervention, thereby ensuring the reproducibility of the results.

    • Oscar Esteban
    • , Christopher J. Markiewicz
    • , Ross W. Blair
    • , Craig A. Moodie
    • , A. Ilkay Isik
    • , Asier Erramuzpe
    • , James D. Kent
    • , Mathias Goncalves
    • , Elizabeth DuPre
    • , Madeleine Snyder
    • , Hiroyuki Oya
    • , Satrajit S. Ghosh
    • , Jessey Wright
    • , Joke Durnez
    • , Russell A. Poldrack
    •  & Krzysztof J. Gorgolewski
  • Article |

    An ex vivo 3D culture model of mycobacterial granulomas recapitulates the in vivo physiology of these structures and enables longitudinal imaging studies. The platform allows genetic and pharmacological manipulation of this key structure.

    • Mark R. Cronan
    • , Molly A. Matty
    • , Allison F. Rosenberg
    • , Landry Blanc
    • , Charlie J. Pyle
    • , Scott T. Espenschied
    • , John F. Rawls
    • , Véronique Dartois
    •  & David M. Tobin
  • Article |

    Imaging of neuronal activity across the whole zebrafish brain in combination with online analysis allows for manipulating neuronal activity according to function. This approach is used to ablate or activate neurons in fictively swimming zebrafish larvae.

    • Nikita Vladimirov
    • , Chen Wang
    • , Burkhard Höckendorf
    • , Avinash Pujala
    • , Masashi Tanimoto
    • , Yu Mu
    • , Chao-Tsung Yang
    • , Jason D. Wittenbach
    • , Jeremy Freeman
    • , Stephan Preibisch
    • , Minoru Koyama
    • , Philipp J. Keller
    •  & Misha B. Ahrens
  • Brief Communication |

    The combination of positive and negative selection strategies, paired with the use of shRNAs to avoid random integration, allows efficient and scarless CRISPR-based homologous recombination.

    • Kazuya Ikeda
    • , Nobuko Uchida
    • , Toshinobu Nishimura
    • , Joseph White
    • , Renata M. Martin
    • , Hiromitsu Nakauchi
    • , Vittorio Sebastiano
    • , Kenneth I. Weinberg
    •  & Matthew H. Porteus
  • Article |

    A particle-filter algorithm for single-particle cryo-electron microscopy, implemented in a tool called THUNDER, provides high-dimensional parameter estimation, improving the obtainable resolution for several protein structures.

    • Mingxu Hu
    • , Hongkun Yu
    • , Kai Gu
    • , Zhao Wang
    • , Huabin Ruan
    • , Kunpeng Wang
    • , Siyuan Ren
    • , Bing Li
    • , Lin Gan
    • , Shizhen Xu
    • , Guangwen Yang
    • , Yuan Shen
    •  & Xueming Li

News & Comment

  • This Month |

    A probe for a journey into bubbles and why it’s good to be both inventor and discoverer.

    • Vivien Marx
  • Comment |

    Inbred mice are preferred over outbred mice because it is assumed that they display less trait variability. We compared coefficients of variation and did not find evidence of greater trait stability in inbred mice. We conclude that contrary to conventional wisdom, outbred mice might be better subjects for most biomedical research.

    • Alexander H. Tuttle
    • , Vivek M. Philip
    • , Elissa J. Chesler
    •  & Jeffrey S. Mogil
  • Research Highlight |

    A new computational method integrates RNA single-cell sequencing and spatial data.

    • Tal Nawy

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