Image: Jonathan Grimm. Cover Design: Marina Spence.

Read the August issue

Latest Research

  • Article |

    Chemically inducible trimerization tools based on split FRB or FKBP with full-length FKBP or FRB, respectively, expand the chemogenetics toolbox. Their efficiency and fast kinetics enable new types of protein manipulation in live cells.

    • Helen D. Wu
    • , Masaki Kikuchi
    • , Onur Dagliyan
    • , Adam K. Aragaki
    • , Hideki Nakamura
    • , Nikolay V. Dokholyan
    • , Takashi Umehara
    •  & Takanari Inoue
  • Article |

    Supervised machine-learning models trained using Drosophila epigenetic and STARR-seq data can be transferred to predict mouse and human enhancers.

    • Anurag Sethi
    • , Mengting Gu
    • , Emrah Gumusgoz
    • , Landon Chan
    • , Koon-Kiu Yan
    • , Joel Rozowsky
    • , Iros Barozzi
    • , Veena Afzal
    • , Jennifer A. Akiyama
    • , Ingrid Plajzer-Frick
    • , Chengfei Yan
    • , Catherine S. Novak
    • , Momoe Kato
    • , Tyler H. Garvin
    • , Quan Pham
    • , Anne Harrington
    • , Brandon J. Mannion
    • , Elizabeth A. Lee
    • , Yoko Fukuda-Yuzawa
    • , Axel Visel
    • , Diane E. Dickel
    • , Kevin Y. Yip
    • , Richard Sutton
    • , Len A. Pennacchio
    •  & Mark Gerstein
  • Article |

    A general tuning strategy is introduced for improving the utility of rhodamines for biological imaging applications. The strategy yielded bright, versatile and bioavailable far-red and near-infrared ‘Janelia Fluor’ dyes.

    • Jonathan B. Grimm
    • , Ariana N. Tkachuk
    • , Liangqi Xie
    • , Heejun Choi
    • , Boaz Mohar
    • , Natalie Falco
    • , Kathy Schaefer
    • , Ronak Patel
    • , Qinsi Zheng
    • , Zhe Liu
    • , Jennifer Lippincott-Schwartz
    • , Timothy A. Brown
    •  & Luke D. Lavis
  • Article |

    OligoFISSEQ combines Oligopaints with fluorescence in situ sequencing to enable the 3D mapping of many regions across the genome in human cells to interrogate genome organization at improved genomic resolution. OligoFISSEQ is compatible with immunochemistry and OligoSTORM for super-resolution imaging.

    • Huy Q. Nguyen
    • , Shyamtanu Chattoraj
    • , David Castillo
    • , Son C. Nguyen
    • , Guy Nir
    • , Antonios Lioutas
    • , Elliot A. Hershberg
    • , Nuno M. C. Martins
    • , Paul L. Reginato
    • , Mohammed Hannan
    • , Brian J. Beliveau
    • , George M. Church
    • , Evan R. Daugharthy
    • , Marc A. Marti-Renom
    •  & C.-ting Wu

News & Comment

  • Editorial |

    We’ve made some recent updates to our various content type offerings at Nature Methods. Here is a cheat sheet.

  • Research Highlight |

    RIC-seq enables in situ mapping of intra- and intermolecular RNA–RNA interactions.

    • Lin Tang
  • Research Highlight |

    barRNAseq allows detailed explorations of signaling pathways that control embryonic differentiation.

    • Madhura Mukhopadhyay
  • Research Highlight |

    Tethering fluorescent proteins to ribosomes clears up background signals when imaging neural circuits.

    • Nina Vogt
  • Research Highlight |

    Direct imaging of carbohydrates using electrospray ion beam deposition and scanning tunneling microscopy.

    • Arunima Singh
  • Research Highlight |

    Intravital three-dimensional bioprinting enables the biofabrication of constructs in a pre-existing 3D matrix, such as inside tissues of live animals.

    • Lei Tang

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

Naturejobs.com

Nature events Directory