Image: Ali Punjani, Haowei Zhang and David J. Fleet. Cover Design: Erin Dewalt.

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Latest Research

  • Article |

    diaPASEF makes use of the correlation between the ion mobility and the m/z of peptides to trap and release precursor ions in a TIMS-TOF mass spectrometer for an almost complete sampling of the precursor ion beam with data-independent acquisition.

    • Florian Meier
    • , Andreas-David Brunner
    • , Max Frank
    • , Annie Ha
    • , Isabell Bludau
    • , Eugenia Voytik
    • , Stephanie Kaspar-Schoenefeld
    • , Markus Lubeck
    • , Oliver Raether
    • , Nicolai Bache
    • , Ruedi Aebersold
    • , Ben C. Collins
    • , Hannes L. Röst
    •  & Matthias Mann
  • Article |

    Using nanopore sequencing as readout, nanoNOMe–seq enables chromosome-level allele-specific profiles of CpG methylation and chromatin accessibility on human cells, in which the chromatin accessibility is profiled through exogenous GpC methylation introduced by a GpC methyltransferase.

    • Isac Lee
    • , Roham Razaghi
    • , Timothy Gilpatrick
    • , Michael Molnar
    • , Ariel Gershman
    • , Norah Sadowski
    • , Fritz J. Sedlazeck
    • , Kasper D. Hansen
    • , Jared T. Simpson
    •  & Winston Timp
  • Article |

    Direct infusion–shotgun proteome analysis (DI-SPA) using data-independent acquisition mass spectrometry (DIA-MS) achieves fast and reproducible results by omitting the liquid-chromatography fractionation step and directly performing gas-phase peptide fractionation by ion mobility.

    • Jesse G. Meyer
    • , Natalie M. Niemi
    • , David J. Pagliarini
    •  & Joshua J. Coon
  • Article |

    A computational tool, Avant-garde, automates refinement of data-independent acquisition mass spectrometry-based quantitative proteomics data.

    • Alvaro Sebastian Vaca Jacome
    • , Ryan Peckner
    • , Nicholas Shulman
    • , Karsten Krug
    • , Katherine C. DeRuff
    • , Adam Officer
    • , Karen E. Christianson
    • , Brendan MacLean
    • , Michael J. MacCoss
    • , Steven A. Carr
    •  & Jacob D. Jaffe
  • Article |

    Engineered deubiquitinases (enDUBs) use nanobodies to bring deubiquitinases to target proteins for selective ubiquitin chain removal. enDUBs can rescue the functional expression of mutant ion channels like those responsible for cystic fibrosis.

    • Scott A. Kanner
    • , Zunaira Shuja
    • , Papiya Choudhury
    • , Ananya Jain
    •  & Henry M. Colecraft

News & Comment

  • Research Highlight |

    High-density microelectrode arrays with dual-mode capabilities can ‘image’ electrical activity in neuronal cultures or slice preparations.

    • Nina Vogt
  • Research Highlight |

    RNA timestamps are molecular recorders that enable one to infer the age of an RNA molecule and record transcriptional activity in a temporal manner.

    • Lei Tang
  • Editorial |

    A new method should be thoroughly tested, applied, described — and peer-reviewed — before biological discoveries generated using the method are published.

  • Research Highlight |

    Technological advances push the limits of single-particle cryo-EM to achieve atomic resolution.

    • Rita Strack
  • This Month |

    A soccer striker and his ion channel rescue tools.

    • Vivien Marx


Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

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