All life sciences research is potentially subject to ethical considerations. Institutions should support collaborations with professional ethicists and philosophers to help life scientists navigate ethical crossroads.

The global imaging community is pursuing innovative approaches to achieve more equitable access to instruments and expertise.

The capability of high-resolution in situ imaging by electron cryo-tomography (cryo-ET) has now been expanded to large multicellular tissues by newly developed workflows involving lift-out and serial sectioning using focused ion beam milling under cryogenic conditions.

This Perspective discusses the integration of small-scale datasets with each other or with larger reference atlases, particularly in the context of single-cell approaches.

This study introduces a chemical method, BACS, that generates Ψ-to-C mutation signatures, allowing for sequencing and quantification of Ψ at single-base resolution.

UnitMatch tracks neurons in electrophysiological recordings across multiple recording sessions. UnitMatch relies on the spike waveform and allows tracking neurons even when their functional properties change such as during memory formation.

Vitessce is a robust and versatile web-based framework for interactive visualization of large-scale multiomics and spatial data at the single-cell level.

This work presents Strainy, a long-read metagenome assembler that allows the identification of strain distributions and mutational patterns in environmental metagenomes.

This Analysis study compares computational methods for single-cell multi-omics prediction and integration, generating useful insights for method users and developers working with different analysis purposes and biological problems.

A neural space–time model can recover a dynamic scene by modeling its spatiotemporal relationship in multi-shot imaging reconstruction for reduced motion artifacts and improved imaging of fast processes in living cells.

Developing new methods takes passion and a penchant for risk-taking.

I don’t have good luck in the match points. —Rafael Nadal, Spanish tennis player.

All life sciences research is potentially subject to ethical considerations. Institutions should support collaborations with professional ethicists and philosophers to help life scientists navigate ethical crossroads.