Image: Shira Friedman. Cover design: Erin Dewalt.

Read the September issue

Latest Research

  • Article |

    Antigen-specific CD8+ T cells can be imaged by immunoPET with the help of synTacs, MHC-based tools that bind to relevant T-cell receptors.

    • Andrew W. Woodham
    • , Stad H. Zeigler
    • , Ella L. Zeyang
    • , Stephen C. Kolifrath
    • , Ross W. Cheloha
    • , Mohammad Rashidian
    • , Rodolfo J. Chaparro
    • , Ronald D. Seidel
    • , Scott J. Garforth
    • , Jason L. Dearling
    • , Maia Mesyngier
    • , Phaneendra K. Duddempudi
    • , Alan B. Packard
    • , Steven C. Almo
    •  & Hidde L. Ploegh
  • Article |

    Tension-PAINT integrates molecular tension probes with DNA-PAINT to enable ~25-nm-resolution mapping of piconewton mechanical events. Tension-PAINT can be used to study dynamic forces, and an irreversible variant integrates force history over time.

    • Joshua M. Brockman
    • , Hanquan Su
    • , Aaron T. Blanchard
    • , Yuxin Duan
    • , Travis Meyer
    • , M. Edward Quach
    • , Roxanne Glazier
    • , Alisina Bazrafshan
    • , Rachel L. Bender
    • , Anna V. Kellner
    • , Hiroaki Ogasawara
    • , Rong Ma
    • , Florian Schueder
    • , Brian G. Petrich
    • , Ralf Jungmann
    • , Renhao Li
    • , Alexa L. Mattheyses
    • , Yonggang Ke
    •  & Khalid Salaita
  • Brief Communication |

    MassIVE.quant is a data repository and data resource for reproducible quantitative mass spectrometry–based proteomics.

    • Meena Choi
    • , Jeremy Carver
    • , Cristina Chiva
    • , Manuel Tzouros
    • , Ting Huang
    • , Tsung-Heng Tsai
    • , Benjamin Pullman
    • , Oliver M. Bernhardt
    • , Ruth Hüttenhain
    • , Guo Ci Teo
    • , Yasset Perez-Riverol
    • , Jan Muntel
    • , Maik Müller
    • , Sandra Goetze
    • , Maria Pavlou
    • , Erik Verschueren
    • , Bernd Wollscheid
    • , Alexey I. Nesvizhskii
    • , Lukas Reiter
    • , Tom Dunkley
    • , Eduard Sabidó
    • , Nuno Bandeira
    •  & Olga Vitek
  • Article |

    Temporal decomposition through manifold fitting (TDM) is an analysis technique that decomposes blood oxygenation level dependent (BOLD) responses in task-based fMRI into different components that likely correspond to microvasculature- and macrovasculature-driven signals.

    • Kendrick Kay
    • , Keith W. Jamison
    • , Ru-Yuan Zhang
    •  & Kamil Uğurbil
  • Article |

    Red and yellow versions of the genetically encoded dopamine sensor dLight1 have been developed and allow multiplexed imaging of dopamine with neurotransmitter or cell-type-specific calcium combined with green sensors or actuators, as demonstrated ex vivo and in behaving rodents.

    • Tommaso Patriarchi
    • , Ali Mohebi
    • , Junqing Sun
    • , Aaron Marley
    • , Ruqiang Liang
    • , Chunyang Dong
    • , Kyle Puhger
    • , Grace Or Mizuno
    • , Carolyn M. Davis
    • , Brian Wiltgen
    • , Mark von Zastrow
    • , Joshua D. Berke
    •  & Lin Tian
  • Article |

    ScNT-seq leverages metabolic labeling of nascent RNA and droplet-based sequencing for parallel analysis of newly transcribed and pre-existing mRNAs, which enables time-resolved analysis of dynamic biological processes in single cells.

    • Qi Qiu
    • , Peng Hu
    • , Xiaojie Qiu
    • , Kiya W. Govek
    • , Pablo G. Cámara
    •  & Hao Wu

News & Comment

  • This Month |

    Frugally built technology to study the ocean’s microbes, and engineering for societal good.

    • Vivien Marx
  • Research Highlight |

    Addition of a second constriction to CsgG-family nanopores improves the accuracy of homopolymer sequencing.

    • Arunima Singh
  • Editorial |

    We distributed an informal questionnaire to learn from scientists about their professional use of WeChat. We share some of their answers and discuss the multipurpose platform offered by WeChat.

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

Naturejobs.com

Nature events Directory