Image: Daniel Castranova, Brant Weinstein & Bakary Samasa. Cover Design: Erin Dewalt.

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Latest Research

  • Article |

    Using nanopore sequencing as readout, nanoNOMe–seq enables chromosome-level allele-specific profiles of CpG methylation and chromatin accessibility on human cells, in which the chromatin accessibility is profiled through exogenous GpC methylation introduced by a GpC methyltransferase.

    • Isac Lee
    • , Roham Razaghi
    • , Timothy Gilpatrick
    • , Michael Molnar
    • , Ariel Gershman
    • , Norah Sadowski
    • , Fritz J. Sedlazeck
    • , Kasper D. Hansen
    • , Jared T. Simpson
    •  & Winston Timp
  • Article |

    Direct infusion–shotgun proteome analysis (DI-SPA) using data-independent acquisition mass spectrometry (DIA-MS) achieves fast and reproducible results by omitting the liquid-chromatography fractionation step and directly performing gas-phase peptide fractionation by ion mobility.

    • Jesse G. Meyer
    • , Natalie M. Niemi
    • , David J. Pagliarini
    •  & Joshua J. Coon
  • Article |

    A computational tool, Avant-garde, automates refinement of data-independent acquisition mass spectrometry-based quantitative proteomics data.

    • Alvaro Sebastian Vaca Jacome
    • , Ryan Peckner
    • , Nicholas Shulman
    • , Karsten Krug
    • , Katherine C. DeRuff
    • , Adam Officer
    • , Karen E. Christianson
    • , Brendan MacLean
    • , Michael J. MacCoss
    • , Steven A. Carr
    •  & Jacob D. Jaffe
  • Article |

    Engineered deubiquitinases (enDUBs) use nanobodies to bring deubiquitinases to target proteins for selective ubiquitin chain removal. enDUBs can rescue the functional expression of mutant ion channels like those responsible for cystic fibrosis.

    • Scott A. Kanner
    • , Zunaira Shuja
    • , Papiya Choudhury
    • , Ananya Jain
    •  & Henry M. Colecraft
  • Article |

    Simultaneous widefield calcium imaging and fMRI provide insight into neural activity at multiple scales and can be used to decipher the cellular origin of BOLD activity.

    • Evelyn M. R. Lake
    • , Xinxin Ge
    • , Xilin Shen
    • , Peter Herman
    • , Fahmeed Hyder
    • , Jessica A. Cardin
    • , Michael J. Higley
    • , Dustin Scheinost
    • , Xenophon Papademetris
    • , Michael C. Crair
    •  & R. Todd Constable

News & Comment

Collection

Expanding the CRISPR Toolbox

The CRISPR-Cas9 system is best known for its ability to knock out or replace specific genes, via targeted cleavage of the genome. But scientists are developing many more applications, typically by using an inactive Cas9 to target other enzymes to specific genomic sites. From transcriptional regulation to base editing, these developments are extending the range of biological questions that can be probed with CRISPR/Cas9.

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