The past year has been long and challenging for all of us. Here’s how Nature Methods has been faring since COVID-19 was declared a pandemic.

Immune cells can transform themselves with plasticity that cancer labs seek to harness.

A deep-learning-guided approach enables protein engineering using only a small number (‘low N’) of functionally characterized variants of target proteins.

PCprophet combines complex-level scoring and machine learning to predict novel protein complexes from protein cofractionation mass spectrometry data and to perform differential analysis across experimental conditions.

Phasor S-FLIM combines novel electronics for multichannel fluorescence lifetime acquisition and a phasor-based unmixing algorithm for real-time analysis of reliable spectral lifetime imaging data, enabling new biological observations.

A deep-learning-guided approach enables protein engineering using only a small number (‘low N’) of functionally characterized variants of target proteins.

An updated version of DIAMOND uses improved algorithmic procedures and a customized high-performance computing framework to make seemingly prohibitive large-scale protein sequence alignments feasible.

The mini-mScope is a miniature microscope that can image neural activity at the mesoscale in most of the dorsal cortex of freely behaving mice.

Bolaños et al. present a realistic three-dimensional virtual mouse model that can be animated and that facilitates the training of pose estimation algorithms.

The split-pool barcoding strategy has been extended to single-cell RNA sequencing (scRNA-seq) of microbes.

Germanium nanoparticles enable optical trapping at high spatiotemporal resolution.

KIN-CLIP allows determination of binding and dissociation kinetics of RNA-binding proteins in cells.

In situ long-read sequencing combined with expansion microscopy enables precise views of transcriptomes in intact biological systems.

Two independent studies describe the reproduction of early events in cardiac development in vitro.

The past year has been long and challenging for all of us. Here’s how Nature Methods has been faring since COVID-19 was declared a pandemic.