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Living bacteria in silica gels

Nature Materials volume 1, pages 42–44 (2002)Cite this article

Abstract

The encapsulation of enzymes within silica gels has been extensively studied during the past decade for the design of biosensors and bioreactors1,2,3,4,5,6,7,8,9,10,11. Yeast spores and bacteria have also been recently immobilized within silica gels12,13,14,15,16,17 where they retain their enzymatic activity, but the problem of the long-term viability of whole cells in an inorganic matrix has never been fully addressed. It is a real challenge for the development of sol–gel processes18. Generic tests have been performed to check the viability of Escherichia coli bacteria in silica gels. Surprisingly, more bacteria remain culturable in the gel than in an aqueous suspension. The metabolic activity of the bacteria towards glycolysis decreases slowly, but half of the bacteria are still viable after one month. When confined within a mineral environment, bacteria do not form colonies. The exchange of chemical signals between isolated bacteria rather than aggregates can then be studied, a point that could be very important for 'quorum sensing'19.

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Figure 1
Figure 2: Determination of the formation of metabolites by 13 C NMR.

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Authors and Affiliations

  1. Chimie de la Matière Condensée, Université Pierre et Marie Curie, 4 place Jussieu, Paris, 75252, France

    Nadine Nassif, Cécile Roux, Thibaud Coradin & Jacques Livage

  2. Unité INSERM 510, Pathogènes et Fonctions Epithéliales Polarisées, Faculté de Pharmacie Paris XI, Chatenay-Malabry, 92296, France

    Nadine Nassif & Odile Bouvet

  3. Service de RMN, UMR 7576, ENSCP, 11 rue P.M. Curie, Paris, 75231, France

    Marie Noelle Rager

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  1. Nadine Nassif
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Correspondence to Jacques Livage.

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Nassif, N., Bouvet, O., Noelle Rager, M. et al. Living bacteria in silica gels. Nature Mater 1, 42–44 (2002). https://doi.org/10.1038/nmat709

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