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A common JAK2 haplotype confers susceptibility to myeloproliferative neoplasms

Nature Genetics volume 41, pages 450–454 (2009)Cite this article

Abstract

Genome-wide association studies have identified a number of new disease susceptibility loci that represent haplotypes defined by numerous SNPs. SNPs within a disease-associated haplotype are thought to influence either the expression of genes or the sequence of the proteins they encode. In a series of investigations of the JAK2 gene in myeloproliferative neoplasms, we uncovered a new property of haplotypes that can explain their disease association. We observed a nonrandom distribution of the somatic JAK2V617F oncogenic mutation between two parental alleles of the JAK2 gene. We identified a haplotype that preferentially acquires JAK2V617F and confers susceptibility to myeloproliferative neoplasms. One interpretation of our results is that a certain combination of SNPs may render haplotypes differentially susceptible to somatic mutagenesis. Thus, disease susceptibility loci may harbor somatic mutations that have a role in disease pathogenesis.

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Figure 1: Cytogenetic analysis of hematopoietic progenitor clones in a subject with primary myelofibrosis.
Figure 2: Detection of multiple acquisitions of the JAK2V617F mutation in exon 14 of the JAK2 gene in MPN.
Figure 3: Association analysis results for eight SNPs from the JAK2 genomic region.
Figure 4: Determination of the JAK2 gene haplotypes that carry JAK2V617F in 17 subjects with uniparental disomy of chromosome 9p (9pUPD).

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Acknowledgements

The study was supported by funding from the Austrian Academy of Sciences, Austrian Science Fund (FWF, P20033-B11) and the Initiative for Cancer Research of the Medical University of Vienna. We thank C. Ay and N. Bachhofner for help with sample collection and T. Burkard for advice on statistical analysis. We thank H. Pickersgill for help with the manuscript.

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Authors and Affiliations

  1. Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria

    Damla Olcaydu, Ashot Harutyunyan, Roland Jäger, Tiina Berg & Robert Kralovics

  2. Department of Internal Medicine I, Division of Hematology and Blood Coagulation, Medical University of Vienna, Vienna, Austria

    Bettina Gisslinger, Ingrid Pabinger, Heinz Gisslinger & Robert Kralovics

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  1. Damla Olcaydu
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  2. Ashot Harutyunyan
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  3. Roland Jäger
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Contributions

R.K. designed the study and drafted the paper with assistance of D.O. and A.H.; D.O., T.B. and R.J. performed the experiments; D.O. and A.H. performed statistical analyses; T.B., B.G., H.G. and I.P. coordinated and performed the case and control sample collection and clinical management of cases.

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Correspondence to Robert Kralovics.

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Olcaydu, D., Harutyunyan, A., Jäger, R. et al. A common JAK2 haplotype confers susceptibility to myeloproliferative neoplasms. Nat Genet 41, 450–454 (2009). https://doi.org/10.1038/ng.341

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