Samples from 19 patients with non-small-cell lung cancer treated with a combination of chemotherapy and immune checkpoint blockade are profiled with single-cell RNA sequencing and spatial transcriptomics to identify factors associated with treatment resistance.

This study introduces AdipoExpress, an eQTL meta-analysis of 2,344 subcutaneous adipose tissue samples, which triples the size of previous studies and expands the discovery of eQTLs colocalized with GWAS signals for cardiometabolic traits.

Genome-wide analysis and genetic manipulation at loci regulated by p53, E2F4 and RFX7 show that convergent promoters with similar epigenetic features can be co-regulated and simultaneously expressed in the same direction.

A gap-free telomere-to-telomere genome assembly for a ram of Hu sheep uncovers genes and variants associated with domestication and selection for wool fineness.

This work presents a framework for estimating cell division numbers using DNA replication-associated polyguanine tract mutations, with applications for understanding tumor natural histories and origins.

This study introduces the concept of Ancestry Components and shows that they can offer improved population stratification correction for geographically correlated traits. By using ancestry-aware polygenic score construction in admixed individuals, the authors find that effect sizes are conserved across ancestry groups.

A long- and short-read barley pan-transcriptome assembled from 20 diverse barley genotypes offers insights into genotype- and tissue-dependent gene expression and function.

Xenium spatial transcriptomic profiling of pulmonary fibrosis characterizes cell composition dynamics and histopathological features associated with the disease.

Simultaneous profiling of the genome, methylome, epigenome and transcriptome using single-molecule chromatin fiber sequencing and multiplexed arrays isoform sequencing identifies the genetic and molecular basis of an undiagnosed Mendelian disease case with an X;13-balanced translocation.

Two-step Mendelian randomization, combined with multiple layers of omics evidence, implicates COL6A3-derived endotrophin as a mediator of coronary artery disease risk in the context of obesity.

This Comment outlines the creation of the Global Alliance for Spatial Technologies (GESTALT), a collaborative initiative aimed at fostering the growth and standardization of spatial tissue profiling technologies. It explores the need for GESTALT, its community-driven structure and its goals, spanning from the immediate to the long term.