Browse Articles

  • Article | | open

    KCNQ1 is a voltage-gated potassium channel that is important in cardiac and epithelial function. Here the authors present a mechanism for KCNQ1 activation and inactivation in which voltage sensor activation promotes pore opening more effectively in the intermediate open state than the fully open state, generating inactivation.

    • Panpan Hou
    • , Jodene Eldstrom
    • , Jingyi Shi
    • , Ling Zhong
    • , Kelli McFarland
    • , Yuan Gao
    • , David Fedida
    •  & Jianmin Cui
  • Article | | open

    Cas9 and Cpf1 have both been adapted for genome engineering, editing and gene expression regulation. Here the authors design a fusion guide RNA that can interact with both proteins for multiple and orthogonal genome manipulation.

    • Jiyeon Kweon
    • , An-Hee Jang
    • , Da-Eun Kim
    • , Jin Wook Yang
    • , Mijung Yoon
    • , Ha Rim Shin
    • , Jin-Soo Kim
    •  & Yongsub Kim
  • Article | | open

    Primase is the specialised DNA-dependent RNA polymerase responsible for the initiation of DNA synthesis during DNA replication. Here the authors use a structural biology approach to identify the initiation site in the S. solfataricus PriSLX primase and to demonstrate that its Fe-S cluster is dispensable for primer synthesis.

    • Sandro Holzer
    • , Jiangyu Yan
    • , Mairi L. Kilkenny
    • , Stephen D. Bell
    •  & Luca Pellegrini
  • Article | | open

    Realising deep-strong coupling phenomena for interacting light-matter systems remains an experimental challenge. Here, Langford et al. employ a circuit quantum electrodynamics chip with moderate coupling between a resonator and transmon qubit to realise digital quantum simulation of deep-strong coupling dynamics.

    • N. K. Langford
    • , R. Sagastizabal
    • , M. Kounalakis
    • , C. Dickel
    • , A. Bruno
    • , F. Luthi
    • , D. J. Thoen
    • , A. Endo
    •  & L. DiCarlo
  • Article | | open

    During glacial periods the oceans stored carbon removed from the atmosphere, yet identifying precisely where that storage occurred remains challenging. Here, the authors show that the deep equatorial Pacific Ocean was a reservoir for respired carbon during glacial periods for at least the last 350 kyr.

    • A. W. Jacobel
    • , J. F. McManus
    • , R. F. Anderson
    •  & G. Winckler
  • Article | | open

    Exploring the helicity-orbital coupling induced skyrmion properties is essential for the spintronic applications. Here the authors report the current controlled skyrmions and antiskyrmions dynamics with locking-unlocking helicity in frustrated magnets by including the dipole-dipole interaction in their model.

    • Xichao Zhang
    • , Jing Xia
    • , Yan Zhou
    • , Xiaoxi Liu
    • , Han Zhang
    •  & Motohiko Ezawa
  • Article | | open

    China has entered a new normal phase of economic development with a changing role in global trade. Here the authors show that emissions embodied in China’s exports declined from 2007 to 2012, while developing countries become the major destinations of China’s export emissions.

    • Zhifu Mi
    • , Jing Meng
    • , Dabo Guan
    • , Yuli Shan
    • , Malin Song
    • , Yi-Ming Wei
    • , Zhu Liu
    •  & Klaus Hubacek
  • Article | | open

    The Labrador Sea drives the circulation of warm water around the North Atlantic’s subpolar gyre, yet the effect it has on European climate remains unclear. Here, the authors present a highly-resolved 3000 year-long reconstruction of gyre strength, and link gyre weakening to European cold periods.

    • Paola Moffa-Sánchez
    •  & Ian R. Hall
  • Article | | open

    Bivalve molluscs have evolved various characteristics to adapt to benthic filter-feeding. Here, Li et al investigate the genome, transcriptomes and proteomes of scallop Chlamys farreri, revealing evidences of molecular adaptations to semi-sessile life and neurotoxins.

    • Yuli Li
    • , Xiaoqing Sun
    • , Xiaoli Hu
    • , Xiaogang Xun
    • , Jinbo Zhang
    • , Ximing Guo
    • , Wenqian Jiao
    • , Lingling Zhang
    • , Weizhi Liu
    • , Jing Wang
    • , Ji Li
    • , Yan Sun
    • , Yan Miao
    • , Xiaokang Zhang
    • , Taoran Cheng
    • , Guoliang Xu
    • , Xiaoteng Fu
    • , Yangfan Wang
    • , Xinran Yu
    • , Xiaoting Huang
    • , Wei Lu
    • , Jia Lv
    • , Chuang Mu
    • , Dawei Wang
    • , Xu Li
    • , Yu Xia
    • , Yajuan Li
    • , Zhihui Yang
    • , Fengliang Wang
    • , Lu Zhang
    • , Qiang Xing
    • , Huaiqian Dou
    • , Xianhui Ning
    • , Jinzhuang Dou
    • , Yangping Li
    • , Dexu Kong
    • , Yaran Liu
    • , Zhi Jiang
    • , Ruiqiang Li
    • , Shi Wang
    •  & Zhenmin Bao
  • Article | | open

    Using CRISPR to write specific genetic sequences can sometimes be difficult due to the preference of mammalian cells to repair breaks using NHEJ. Here the authors form nanoparticles to localize the template sequence to the nuclease, shifting repair in favor of HDR.

    • Jared Carlson-Stevermer
    • , Amr A. Abdeen
    • , Lucille Kohlenberg
    • , Madelyn Goedland
    • , Kaivalya Molugu
    • , Meng Lou
    •  & Krishanu Saha
  • Article | | open

    Phase modifications take place in electron transport through quantum dots, yet the details are not agreed between theory and experimental observations to date. Edlbauer et al. confirm the theory by scanning fourteen consecutive resonant states of a large quantum dot hosting hundreds of electrons.

    • Hermann Edlbauer
    • , Shintaro Takada
    • , Grégoire Roussely
    • , Michihisa Yamamoto
    • , Seigo Tarucha
    • , Arne Ludwig
    • , Andreas D. Wieck
    • , Tristan Meunier
    •  & Christopher Bäuerle
  • Article | | open

    The magnitude of organic carbon burial in lakes and reservoirs is poorly constrained. Here, using a compilation of modern data from the literature and statistical modeling, the authors estimate a global yearly organic carbon burial of 0.15 Pg C in inland waters, of which 40% is stored in reservoirs.

    • Raquel Mendonça
    • , Roger A. Müller
    • , David Clow
    • , Charles Verpoorter
    • , Peter Raymond
    • , Lars J. Tranvik
    •  & Sebastian Sobek
  • Article | | open

    Complement component C1q activates efferocytosis, suppresses inflammatory responses, and is thereby thought to limit autoimmune disease. Here, the authors show that macrophage transcription factor MafB regulates total serum levels of C1q, which contributes to preventing autoimmune disease in mice.

    • Mai Thi Nhu Tran
    • , Michito Hamada
    • , Hyojung Jeon
    • , Risako Shiraishi
    • , Keigo Asano
    • , Motochika Hattori
    • , Megumi Nakamura
    • , Yuki Imamura
    • , Yuki Tsunakawa
    • , Risa Fujii
    • , Toshiaki Usui
    • , Kaushalya Kulathunga
    • , Christina-Sylvia Andrea
    • , Ryusuke Koshida
    • , Risa Kamei
    • , Yurina Matsunaga
    • , Makoto Kobayashi
    • , Hisashi Oishi
    • , Takashi Kudo
    •  & Satoru Takahashi
  • Article | | open

    Human indoleamine 2,3-dioxygenase 1 (hIDO1) is an immunotherapeutic target for cancer therapy. Here, the authors present the substrate-, inhibitor- and effector-bound hIDO1 crystal structures, which give insights into the mechanism and reveal a second small molecule binding site, which is of interest for drug design.

    • Ariel Lewis-Ballester
    • , Khoa N. Pham
    • , Dipanwita Batabyal
    • , Shay Karkashon
    • , Jeffrey B. Bonanno
    • , Thomas L. Poulos
    •  & Syun-Ru Yeh
  • Article | | open

    Extracellular vesicles (EV)-associated RNAs are serum biomarkers potentially exploitable for early cancer diagnosis. Here the authors develop a catalyzed hairpin DNA circuit within a cationic lipid-polymer hybrid nanoparticle that can detect low–level EV-associated RNAs in early stage cancer patients.

    • Jiaming Hu
    • , Yan Sheng
    • , Kwang Joo Kwak
    • , Junfeng Shi
    • , Bohao Yu
    •  & L. James Lee
  • Article | | open

    It is unclear whether the transfer of plasmids carrying antibiotic resistance genes can explain their persistence when antibiotics are not present. Here, Lopatkin et al. show that conjugal plasmids, even when costly, are indeed transferred at sufficiently high rates to be maintained in the absence of antibiotics.

    • Allison J. Lopatkin
    • , Hannah R. Meredith
    • , Jaydeep K. Srimani
    • , Connor Pfeiffer
    • , Rick Durrett
    •  & Lingchong You
  • Article | | open

    Cachexia is a metabolic syndrome leading to muscle and adipose tissue loss in majority of cancer patients. Here the authors show that, in a mouse model, BET inhibitor JQ1 counteracts muscle and adipose tissue wasting tempering cachexia and prolonging survival through a mechanism unrelated to tumour growth.

    • Marco Segatto
    • , Raffaella Fittipaldi
    • , Fabrizio Pin
    • , Roberta Sartori
    • , Kyung Dae Ko
    • , Hossein Zare
    • , Claudio Fenizia
    • , Gianpietro Zanchettin
    • , Elisa Sefora Pierobon
    • , Shinji Hatakeyama
    • , Cosimo Sperti
    • , Stefano Merigliano
    • , Marco Sandri
    • , Panagis Filippakopoulos
    • , Paola Costelli
    • , Vittorio Sartorelli
    •  & Giuseppina Caretti
  • Article | | open

    Synchronous neural activity is related with memory encoding and retrieval, but it is not clear whether this happens across the whole brain. Here, authors use intracranial recordings to show that gamma networks are largely asynchronous, desynchronizing while theta synchronizes during memory encoding and retrieval.

    • E. A. Solomon
    • , J. E. Kragel
    • , M. R. Sperling
    • , A. Sharan
    • , G. Worrell
    • , M. Kucewicz
    • , C. S. Inman
    • , B. Lega
    • , K. A. Davis
    • , J. M. Stein
    • , B. C. Jobst
    • , K. A. Zaghloul
    • , S. A. Sheth
    • , D. S. Rizzuto
    •  & M. J. Kahana
  • Article | | open

    Tip-growing cells can find their growing path toward the source of attractive signals. Here, using experimental data and mathematical modeling, Luo et al. demonstrate that tip-localized exocytosis can integrate guidance cues with Rho GTPase signaling to control cell wall mechanics and direct tip growth in Arabidopsis pollen tubes.

    • Nan Luo
    • , An Yan
    • , Gang Liu
    • , Jingzhe Guo
    • , Duoyan Rong
    • , Masahiro M. Kanaoka
    • , Zhen Xiao
    • , Guanshui Xu
    • , Tetsuya Higashiyama
    • , Xinping Cui
    •  & Zhenbiao Yang
  • Article | | open

    Drosophila Polo kinase is the founding member of the Polo-Like Kinase (PLK) family and a master regulator of mitosis and cytokinesis. Here the authors uncover a molecular mechanism for the spatiotemporal regulation of Polo kinase during mitotic entry through a phosphorylation event that triggers nuclear import.

    • David Kachaner
    • , Damien Garrido
    • , Haytham Mehsen
    • , Karine Normandin
    • , Hugo Lavoie
    •  & Vincent Archambault
  • Article | | open

    Sortilin is an endocytosis receptor with a luminal β-propeller domain. Here the authors present the structures of the β-propeller domain at neutral and acidic pH, which reveal that sortilin dimerises and undergoes conformational changes at low pH and further propose a model for low pH-induced ligand release by endocytosis receptors.

    • Nadia Leloup
    • , Philip Lössl
    • , Dimphna H. Meijer
    • , Martha Brennich
    • , Albert J. R. Heck
    • , Dominique M. E. Thies-Weesie
    •  & Bert J. C. Janssen
  • Article | | open

    Checkpoint kinase 1 (CHK1) is critical for intrinsic cell cycle control and coordination of cell cycle progression. Here the authors show that CHK1 loss or chemical inhibition impacts on normal B cell development, lymphomagenesis and cancer cell survival.

    • Fabian Schuler
    • , Johannes G. Weiss
    • , Silke E. Lindner
    • , Michael Lohmüller
    • , Sebastian Herzog
    • , Simon F. Spiegl
    • , Philipp Menke
    • , Stephan Geley
    • , Verena Labi
    •  & Andreas Villunger
  • Article | | open

    β1 and β2 adrenergic receptors (ARs) are the predominant G protein-coupled receptor subtypes expressed in mammalian hearts. Here the authors describe a signaling mechanism where the biased agonist carvedilol converts β1AR from a classical Gαs-coupled to a Gαi-coupled receptor to mediate β-arrestin-dependent signaling.

    • Jialu Wang
    • , Kenji Hanada
    • , Dean P. Staus
    • , Michael A. Makara
    • , Giri Raj Dahal
    • , Qiang Chen
    • , Andrea Ahles
    • , Stefan Engelhardt
    •  & Howard A. Rockman