Building on a century of enzymology research and new genome-wide insights into enzyme families, increased interdisciplinary communication and a broad vision will advance our understanding of biological catalysts and enhance our ability to manipulate them.
Volume 5 Issue 8, August 2009
The 2009 ESBOC meeting covered advances in synthesis, biosynthesis and biological mechanisms of vitamins and cofactors. Recent exciting developments in the field include the development of 'green' chemistry for manufacture of vitamins, the discovery that some vitamins act directly to regulate gene expression via riboswitches, and initial attempts to exploit the potential of vitamin analogs as therapeutic drugs.
News & Views
The parallel determination of more than 100 absolute intracellular metabolite concentrations by isotope-labeled HPLC-MS allowed a comprehensive analysis of the cellular state, its metabolic capacities and modes of regulation. Among other findings, it became obvious that for most enzymes the concentrations were above the Km values, which indicates a trend toward saturation of most enzyme active sites.
Random mutations usually destabilize protein tertiary structure. Overexpression of the chaperonin GroEL/GroES protects evolving proteins from the destabilizing effects of adaptive mutations and improves the quantity and quality of enzyme variants with modified function.
In This Issue
Insights gained from advanced techniques and large datasets are charting new courses in enzymological research. In this issue, we feature a collection of articles highlighting the current ideas and future challenges that are redirecting research in the field.