Volume 20

  • No. 7 July 2024

    Tuning Cas kinetics for one-pot testing

    Tong et al. found that the cleavage kinetics of Cas effectors need to be precisely tuned to maximize their sensitivity in one-pot nucleic acid detection. The image exemplifies the synchronized interplay between substrate amplification and cleavage by Cas effectors.

    See Tong et al.

  • No. 6 June 2024

    Lighting up proteins by RNA editing

    A method termed RENAPT combines RNA editing and site-specific incorporation of non-canonical amino acids, enabling introduction of small chemical tags into endogenous proteins for live-cell imaging. The cover depicts a super-resolution image of the GRP94 protein, an endoplasmic reticulum-resident chaperone (in red), achieved through RENAPT.

    See Hao et al. and Doura et al.

  • No. 5 May 2024

    A checkpoint for insulin secretion

    A chemical screen reveals that inhibitors of CHEK2, a checkpoint kinase, can enhance glucose-stimulated insulin secretion in human primary islets and animal models. The cover shows a confocal microscopy image of a human islet immunostained with antibodies against insulin (green), Ki67 (red) and DAPI (blue).

    See Chong et al.

  • No. 4 April 2024

    Putting the brakes on dynein

    Cytoplasmic dynein is a motor complex that transports intracellular cargos toward the minus end of microtubules. The image depicts Lis1 binding to the dynein motor domain to facilitate the assembly of active dynein complexes, leading to a slower transport speed.

    See Kusakci et al.

  • No. 3 March 2024

    Concentrated by condensates

    Small molecules can concentrate in diverse cellular compartments. The image, captured by two-photon microscopy, shows the accumulation of tryptanthrin, a quinazoline indole alkaloid and active ingredient in medicinal herbs, within biomolecular condensates and cytoplasmic organelles.

    See Kilgore et al.

  • No. 2 February 2024

    Peaks and valleys of GPCR signaling

    The spatial organization of GPCRs is closely associated with their signaling responses and cellular function. Kockelkoren et al. reveal that the spatial organization is energetic coupling of receptors to the curvature of the plasma membrane. The image depicts the landscape of GPCR distribution at the plasma membrane of living cells.

    See Kockelkoren et al.

  • No. 1 January 2024

    Living with lipid mimics

    Genetic code expansion (GCE) techniques are valuable for studying post-translational modifications by incorporating modified non-canonical amino acids into specific sites within target proteins. The image depicts lipidated proteins produced via GCE anchored to the membrane.

    See Ding et al.