Volume 14 Issue 8, August 2018

Volume 14 Issue 8

Diversity in bloom

In Rauwolfia serpentina (pictured) and other alkaloid-producing plants, sarpagan bridge enzyme can catalyze either cyclization or aromatization, depending on the substrate, to produce diverse classes of monoterpene indole alkaloid products.

See O’Connor et al.

Image: Dinodia Photos/Alamy Stock Photo. Cover Design: Erin Dewalt.

Research Highlights

News & Views

  • News & Views |

    Adaptive survival signaling can promote resistance to individual kinase inhibitors. A new study used a pathway-based approach to characterize shared kinome-wide rewiring mechanisms across multiple kinase inhibitors and developed rational drug-combination approaches that target cross-talk between two signaling pathways.

    • Vinayak Palve
    • , Brent M. Kuenzi
    •  & Uwe Rix
  • News & Views |

    Monitoring MHC-I dynamics upon binding to its chaperone TAPBPR helps us understand how optimal peptide sequences are selected for presentation and coordinated with release of the chaperone from the ternary peptide–MHC-I–TAPBPR complex.

    • Tim Elliott
  • News & Views |

    Calcium channels are crucial regulators of a broad range of biological processes. A photoswitchable chemical probe allows the opening and closing of these channels with unprecedented temporal resolution, providing new opportunities to study calcium-dependent signaling pathways in real time.

    • Marc Vendrell

Perspectives

Brief Communications

  • Brief Communication |

    Three homologous cytochrome P450s from monoterpene indole alkaloid-producing plants enable the identification of sarpagan bridge enzyme, which catalyzes either cyclization or aromatization to yield sarpagan or β-carboline alkaloids, respectively.

    • Thu-Thuy T. Dang
    • , Jakob Franke
    • , Ines Soares Teto Carqueijeiro
    • , Chloe Langley
    • , Vincent Courdavault
    •  & Sarah E. O’Connor
  • Brief Communication |

    A photoswitchable probe to control Ca2+ influx through L-type Ca2+ channels is useful in pancreatic β cells and can be employed to modulate beating rate in explanted hearts.

    • Timm Fehrentz
    • , Florian M. E. Huber
    • , Nina Hartrampf
    • , Tobias Bruegmann
    • , James A. Frank
    • , Nicholas H. F. Fine
    • , Daniela Malan
    • , Johann G. Danzl
    • , Denis B. Tikhonov
    • , Martin Sumser
    • , Philipp Sasse
    • , David J. Hodson
    • , Boris S. Zhorov
    • , Nikolaj Klöcker
    •  & Dirk Trauner

Articles

  • Article |

    Proteomic mapping of dynamic changes in kinase signaling after drug treatment identifies that AURKA inhibition is required for drug sensitivity, representing a new co-targeting opportunity with PI3K, AKT, or mTOR inhibitors in breast cancer.

    • Hayley J. Donnella
    • , James T. Webber
    • , Rebecca S. Levin
    • , Roman Camarda
    • , Olga Momcilovic
    • , Nora Bayani
    • , Khyati N. Shah
    • , James E. Korkola
    • , Kevan M. Shokat
    • , Andrei Goga
    • , John D. Gordan
    •  & Sourav Bandyopadhyay
  • Article |

    The membrane-bound enzyme CybB can directly oxidize superoxide to molecular oxygen and transfer the sequestered electrons to ubiquinone in vitro, providing a mechanism for superoxide scavenging distinct from that of superoxide dismutase.

    • Camilla A. K. Lundgren
    • , Dan Sjöstrand
    • , Olivier Biner
    • , Matthew Bennett
    • , Axel Rudling
    • , Ann-Louise Johansson
    • , Peter Brzezinski
    • , Jens Carlsson
    • , Christoph von Ballmoos
    •  & Martin Högbom
  • Article |

    Efficient production of a simple carbapenem antibiotic in Escherichia coli is achieved by a combination of feedback-resistant enzymes for increased precursor biosynthesis and inhibition of fatty acid synthesis for tolerance toward the toxic product.

    • Helena Shomar
    • , Sophie Gontier
    • , Niels J. F. van den Broek
    • , Héctor Tejeda Mora
    • , Marek J. Noga
    • , Peter-Leon Hagedoorn
    •  & Gregory Bokinsky
  • Article |

    A Rab11 FRET biosensor reveals a spatiotemporal interplay between Rab11 and PtdIns(3)P turnover during the removal of recycling cargo from the endosome.

    • Carlo Cosimo Campa
    • , Jean Piero Margaria
    • , Abhishek Derle
    • , Marco Del Giudice
    • , Maria Chiara De Santis
    • , Luca Gozzelino
    • , Francesca Copperi
    • , Carla Bosia
    •  & Emilio Hirsch
  • Article |

    Use of NMR to monitor MHC-I dynamics upon binding to the MHC-I chaperone TAPBPR explains the selection of optimal peptide sequences and release of the chaperone from the ternary peptide-MHC-I–TAPBPR complex.

    • Andrew C. McShan
    • , Kannan Natarajan
    • , Vlad K. Kumirov
    • , David Flores-Solis
    • , Jiansheng Jiang
    • , Mareike Badstübner
    • , Jugmohit S. Toor
    • , Clive R. Bagshaw
    • , Evgenii L. Kovrigin
    • , David H. Margulies
    •  & Nikolaos G. Sgourakis
  • Article |

    Synthetic microbial consortia were engineered as experimental models of bacterial interactions within ecosystems, and mathematical models of their behavior were used to design more complex microbial systems with additional interactions.

    • Wentao Kong
    • , David R. Meldgin
    • , James J. Collins
    •  & Ting Lu