Latest Research

  • Article |

    Screening for substrate preference of the SARS-CoV and SARS-CoV-2 main protease Mpro leads to the development of activity-based probes useful for structural analysis and for visualization of active Mpro in infected patient epithelial cells.

    • Wioletta Rut
    • , Katarzyna Groborz
    • , Linlin Zhang
    • , Xinyuanyuan Sun
    • , Mikolaj Zmudzinski
    • , Bartlomiej Pawlik
    • , Xinyu Wang
    • , Dirk Jochmans
    • , Johan Neyts
    • , Wojciech Młynarski
    • , Rolf Hilgenfeld
    •  & Marcin Drag
  • Article |

    A screening approach finds VH-domain antibodies that bind the SARS-CoV-2 Spike protein receptor-binding domain at its interface with host ACE2. Bi-paratopic and multivalent binders have high affinity and potency.

    • Colton J. Bracken
    • , Shion A. Lim
    • , Paige Solomon
    • , Nicholas J. Rettko
    • , Duy P. Nguyen
    • , Beth Shoshana Zha
    • , Kaitlin Schaefer
    • , James R. Byrnes
    • , Jie Zhou
    • , Irene Lui
    • , Jia Liu
    • , Katarina Pance
    • , Caleigh M. Azumaya
    • , Julian R. Braxton
    • , Axel F. Brilot
    • , Meghna Gupta
    • , Fei Li
    • , Kyle E. Lopez
    • , Arthur Melo
    • , Gregory E. Merz
    • , Frank Moss
    • , Joana Paulino
    • , Thomas H. Pospiech Jr.
    • , Sergei Pourmal
    • , Cristina Puchades
    • , Alexandrea N. Rizo
    • , Amber M. Smith
    • , Ming Sun
    • , Paul V. Thomas
    • , Feng Wang
    • , Zanlin Yu
    • , Daniel Asarnow
    • , Julian R. Braxton
    • , Melody G. Campbell
    • , Cynthia M. Chio
    • , Un Seng Chio
    • , Miles Sasha Dickinson
    • , Devan Diwanji
    • , Bryan Faust
    • , Meghna Gupta
    • , Nick Hoppe
    • , Mingliang Jin
    • , Fei Li
    • , Junrui Li
    • , Yanxin Liu
    • , Gregory E. Merz
    • , Henry C. Nguyen
    • , Joana Paulino
    • , Thomas H. Pospiech Jr.
    • , Sergei Pourmal
    • , Smriti Sangwan
    • , Raphael Trenker
    • , Donovan Trinidad
    • , Eric Tse
    • , Kaihua Zhang
    • , Fengbo Zhou
    • , Caleigh M. Azumaya
    • , Christian Billesboelle
    • , Alisa Bowen
    • , Melody G. Campbell
    • , Devan Diwanji
    • , Nick Hoppe
    • , Yen-Li Li
    • , Phuong Nguyen
    • , Carlos Nowotny
    • , Cristina Puchades
    • , Mali Safari
    • , Smriti Sangwan
    • , Kaitlin Schaefer
    • , Amber M. Smith
    • , Raphael Trenker
    • , Tsz Kin Martin Tsui
    • , Natalie Whitis
    • , Jianhua Zhao
    • , Daniel Asarnow
    • , Caleigh M. Azumaya
    • , Cynthia M. Chio
    • , Bryan Faust
    • , Meghna Gupta
    • , Kate Kim
    • , Michelle Moritz
    • , Tristan W. Owens
    • , Joana Paulino
    • , Jessica K. Peters
    • , Sergei Pourmal
    • , Kaitlin Schaefer
    • , Tsz Kin Martin Tsui
    • , Justin Biel
    • , Ishan Deshpande
    • , Nadia Herrera
    • , Huong T. Kratochvil
    • , Xi Liu
    • , Ursula Schulze-Gahmen
    • , Iris D. Young
    • , Jen Chen
    • , Amy Diallo
    • , Loan Doan
    • , Sebastian Flores
    • , Meghna Gupta
    • , Mingliang Jin
    • , Huong T. Kratochvil
    • , Victor L. Lam
    • , Yang Li
    • , Megan Lo
    • , Gregory E. Merz
    • , Joana Paulino
    • , Aye C. Thwin
    • , Erron W. Titus
    • , Zanlin Yu
    • , Fengbo Zhou
    • , Yang Zhang
    • , David Bulkley
    • , Arceli Joves
    • , Almarie Joves
    • , Liam McKay
    • , Mariano Tabios
    • , Eric Tse
    • , David A. Agard
    • , Yifan Cheng
    • , James S. Fraser
    • , Adam Frost
    • , Natalia Jura
    • , Tanja Kortemme
    • , Nevan J. Krogan
    • , Aashish Manglik
    • , Oren S. Rosenberg
    • , Daniel R. Southworth
    • , Robert M. Stroud
    • , Kliment A. Verba
    • , Xin X. Zhou
    • , Kevin K. Leung
    •  & James A. Wells
  • Article |

    The plant cuticle was initially thought to act as a passive diffusion barrier. Genetic and metabolic analysis reveals that it is also a sink/concentrator for volatiles protecting cells from toxic effects of these hydrophobic compounds.

    • Pan Liao
    • , Shaunak Ray
    • , Benoît Boachon
    • , Joseph H. Lynch
    • , Arnav Deshpande
    • , Scott McAdam
    • , John A. Morgan
    •  & Natalia Dudareva
  • Review Article |

    This Review summarizes the chemical and physical properties of methylated nucleobases in DNA and RNA, proposes a chemical classification of methylation types, and discusses recent advance in demethylation reactions mediated by dioxygenases.

    • Guo-Liang Xu
    •  & Matthias Bochtler
  • Article |

    Increased production of (S)-reticuline and other alkaloids is achieved through alleviating norcoclaurine synthase toxicity by targeting the enzyme to the peroxisome plus enlarging peroxisomes by expression of engineered transcription factors.

    • Parbir S. Grewal
    • , Jennifer A. Samson
    • , Jordan J. Baker
    • , Brian Choi
    •  & John E. Dueber
  • Article |

    A family of riboswitches named Sensei that specifically sense iron has been discovered and characterized, which enables the authors to engineer metal ion sensing riboswitches to convert their metal selectivity.

    • Siladitya Bandyopadhyay
    • , Susmitnarayan Chaudhury
    • , Dolly Mehta
    •  & Arati Ramesh
  • Article |

    A chemical glycobiology approach reveals that heparan-sulfate glycosaminoglycans regulate vascular development through direct interactions with angiopoietin (Ang) ligands and the Tie1 receptor of the Ang–Tie signaling system.

    • Matthew E. Griffin
    • , Alexander W. Sorum
    • , Gregory M. Miller
    • , William A. Goddard III
    •  & Linda C. Hsieh-Wilson
  • Article |

    Structural analysis of transcription activation complex comprising E. coli transcription factor CueR, RNAP holoenzyme and promoter DNA reveals that CueR distorts the DNA conformation to promote the association of promoter with polymerase.

    • Chengli Fang
    • , Steven J. Philips
    • , Xiaoxian Wu
    • , Kui Chen
    • , Jing Shi
    • , Liqiang Shen
    • , Juncao Xu
    • , Yu Feng
    • , Thomas V. O’Halloran
    •  & Yu Zhang
  • Article |

    The whitefly Bemisia tabaci defends against plant glucosinolate toxins by serial addition of glucose moieties catalyzed by a pair of glycoside hydrolases, preventing toxin activation during feeding on the plant tissue.

    • Osnat Malka
    • , Michael L. A. E. Easson
    • , Christian Paetz
    • , Monika Götz
    • , Michael Reichelt
    • , Beate Stein
    • , Katrin Luck
    • , Aleksa Stanišić
    • , Ksenia Juravel
    • , Diego Santos-Garcia
    • , Lilach L. Mondaca
    • , Simon Springate
    • , John Colvin
    • , Stephan Winter
    • , Jonathan Gershenzon
    • , Shai Morin
    •  & Daniel G. Vassão
  • Article |

    A fluorescence-based sensor of PKA activity has increased brightness, dynamic range and signal-to-noise ratio over related sensors and is useful for visualizing kinase activity in HeLa cells, primary neurons and the cortex of awake mice.

    • Jin-Fan Zhang
    • , Bian Liu
    • , Ingie Hong
    • , Albert Mo
    • , Richard H. Roth
    • , Brian Tenner
    • , Wei Lin
    • , Jason Z. Zhang
    • , Rosana S. Molina
    • , Mikhail Drobizhev
    • , Thomas E. Hughes
    • , Lin Tian
    • , Richard L. Huganir
    • , Sohum Mehta
    •  & Jin Zhang
  • Article |

    Native ion mobility mass spectrometry reveals two isoforms of the two-pore domain K+ channel K2P4.1 have distinct binding preferences for lipids and show a relationship between the strength of individual lipid binding events and channel activity.

    • Samantha Schrecke
    • , Yun Zhu
    • , Jacob W. McCabe
    • , Mariah Bartz
    • , Charles Packianathan
    • , Minglei Zhao
    • , Ming Zhou
    • , David Russell
    •  & Arthur Laganowsky
  • Article |

    A bifunctional AURORA-A degrader induces the fast and specific degradation of this kinase in cancer cell lines, which enables targeting of non-catalytic, oncogenic functions of AURORA-A resulting in S-phase arrest and rampant apoptosis.

    • Bikash Adhikari
    • , Jelena Bozilovic
    • , Mathias Diebold
    • , Jessica Denise Schwarz
    • , Julia Hofstetter
    • , Martin Schröder
    • , Marek Wanior
    • , Ashwin Narain
    • , Markus Vogt
    • , Nevenka Dudvarski Stankovic
    • , Apoorva Baluapuri
    • , Lars Schönemann
    • , Lorenz Eing
    • , Pranjali Bhandare
    • , Bernhard Kuster
    • , Andreas Schlosser
    • , Stephanie Heinzlmeir
    • , Christoph Sotriffer
    • , Stefan Knapp
    •  & Elmar Wolf
  • Article |

    Immunomodulatory drugs are used for the treatment of multiple myeloma. ARID2, a component of the PBAF chromatin-remodeling complex, is a new pomalidomide-induced neosubstrate of CRL4CRBN, which accounts for its superior efficacy over lenalidomide.

    • Junichi Yamamoto
    • , Tetsufumi Suwa
    • , Yuki Murase
    • , Shumpei Tateno
    • , Hirotaka Mizutome
    • , Tomoko Asatsuma-Okumura
    • , Nobuyuki Shimizu
    • , Tsutomu Kishi
    • , Shuji Momose
    • , Masahiro Kizaki
    • , Takumi Ito
    • , Yuki Yamaguchi
    •  & Hiroshi Handa
  • Article |

    Imaging of phosphatidylcholine, sphingomyelin and their interorganelle lipid transport in live cells, using azido-choline and a spatially limited bioorthogonal tag, suggests that autophagosomal membranes originate from the ER.

    • Tomonori Tamura
    • , Alma Fujisawa
    • , Masaki Tsuchiya
    • , Yuying Shen
    • , Kohjiro Nagao
    • , Shin Kawano
    • , Yasushi Tamura
    • , Toshiya Endo
    • , Masato Umeda
    •  & Itaru Hamachi
  • Review Article |

    Virulence factors from various bacterial pathogens catalyze post-translational modification of key host proteins, including phosphate β-elimination and phosphoribosyl-linked ubiquitination, to suppress host immune defenses and promote infection.

    • Kaitlin A. Chambers
    •  & Rebecca A. Scheck
  • Article |

    Cryo-EM structural work shows sterols binding at four adjacent locations within the class F GPCR Smoothened (SMO), where the transmembrane core functions as a sterol tunnel in which occupancy activates SMO for downstream Hedgehog signaling.

    • Xiaofeng Qi
    • , Lucas Friedberg
    • , Ryan De Bose-Boyd
    • , Tao Long
    •  & Xiaochun Li
  • Article |

    Incorporation of the non-canonical amino acid 3-aminotyrosine into the chromophores of green fluorescent protein-based biosensors systematically red-shifts their fluorescent properties while maintaining brightness, dynamic range and responsiveness.

    • Shen Zhang
    •  & Hui-wang Ai
  • Article |

    Pyruvate-responsive circuits based on an orthologous transcription factor and adaptation of an antisense transcriptional circuit were developed to sense pyruvate in Bacillus subtilis and redirect metabolism for optimized glucaric acid production.

    • Xianhao Xu
    • , Xueliang Li
    • , Yanfeng Liu
    • , Yonglian Zhu
    • , Jianghua Li
    • , Guocheng Du
    • , Jian Chen
    • , Rodrigo Ledesma-Amaro
    •  & Long Liu
  • Article |

    Reengineering of the lac operon in E. coli from a ligand-inducible to a blue-light-regulated gene expression system facilitates optogenetic control of biotechnological applications including metabolic engineering and protein expression.

    • Makoto A. Lalwani
    • , Samantha S. Ip
    • , César Carrasco-López
    • , Catherine Day
    • , Evan M. Zhao
    • , Hinako Kawabe
    •  & José L. Avalos
  • Article |

    An irreversible small-molecule inhibitor of histone methyltransferase NSD1 is developed, which binds covalently to the C2062 residue in the catalytic SET domain and represses H3K36 dimethylation and target gene expression in leukemia cells.

    • Huang Huang
    • , Christina A. Howard
    • , Sergei Zari
    • , Hyo Je Cho
    • , Shirish Shukla
    • , Hao Li
    • , Juliano Ndoj
    • , Paula González-Alonso
    • , Caroline Nikolaidis
    • , Joshua Abbott
    • , David S. Rogawski
    • , Mykhaylo A. Potopnyk
    • , Katarzyna Kempinska
    • , Hongzhi Miao
    • , Trupta Purohit
    • , Andrew Henderson
    • , Anna Mapp
    • , Maria Luisa Sulis
    • , Adolfo Ferrando
    • , Jolanta Grembecka
    •  & Tomasz Cierpicki
  • Article |

    Structural and biochemical analysis of E. coli transketolase with 2′-methoxy-thiamine shows that this antivitamin selectively inhibits the bacterial enzyme via a steric clash with a critical glutamate residue, preventing cofactor activation.

    • Fabian Rabe von Pappenheim
    • , Matteo Aldeghi
    • , Brateen Shome
    • , Tadhg Begley
    • , Bert L. de Groot
    •  & Kai Tittmann
  • Article |

    A cell-free system for cannabinoid production uses only low-cost inputs with 12 enzymes and can operate either aerobically or anaerobically, in addition to reducing ATP requirements by use of an engineered system for malonate-CoA biosynthesis.

    • Meaghan A. Valliere
    • , Tyler P. Korman
    • , Mark A. Arbing
    •  & James U. Bowie
  • Article |

    Cryo-EM structural work defines binding of the insecticide CHL in the pseudo-voltage-sensor domain of ryanodine receptor RyR that triggers conformational changes leading to channel opening and explains the resistance to CHL by some insects.

    • Ruifang Ma
    • , Omid Haji-Ghassemi
    • , Dan Ma
    • , Heng Jiang
    • , Lianyun Lin
    • , Li Yao
    • , Arthur Samurkas
    • , Yuxin Li
    • , Yiwen Wang
    • , Peng Cao
    • , Shian Wu
    • , Yan Zhang
    • , Takashi Murayama
    • , Bernard Moussian
    • , Filip Van Petegem
    •  & Zhiguang Yuchi
  • Article |

    A chemical screen targeting major epigenetic pathways identifies permissive epigenetic states that enable reprogramming with a broad range of transcriptional regulators and almost all octamer-binding (OCT) family members.

    • Kee-Pyo Kim
    • , Jinmi Choi
    • , Juyong Yoon
    • , Jan M. Bruder
    • , Borami Shin
    • , Jonghun Kim
    • , Marcos J. Arauzo-Bravo
    • , Dong Han
    • , Guangming Wu
    • , Dong Wook Han
    • , Johnny Kim
    • , Patrick Cramer
    •  & Hans R. Schöler
  • Article |

    The interaction between E1 and E2 is targeted by a stapled peptide that mimics the alpha-1 helix of E2, resulting in blockade of ubiquitin transfer from E1 to E2.

    • Ann M. Cathcart
    • , Gregory H. Bird
    • , Thomas E. Wales
    • , Henry D. Herce
    • , Edward P. Harvey
    • , Zachary J. Hauseman
    • , Catherine E. Newman
    • , Utsarga Adhikary
    • , Michelle S. Prew
    • , Tun Oo
    • , Susan Lee
    • , John R. Engen
    •  & Loren D. Walensky
  • Article |

    Structural and biophysical approaches suggest that structural preorganization is important for triggering endogenous CD8+ T cells and escape from immune tolerance, as demonstrated by a single nonsynonymous mutation in an ovarian cancer neoepitope

    • Jason R. Devlin
    • , Jesus A. Alonso
    • , Cory M. Ayres
    • , Grant L. J. Keller
    • , Sara Bobisse
    • , Craig W. Vander Kooi
    • , George Coukos
    • , David Gfeller
    • , Alexandre Harari
    •  & Brian M. Baker
  • Article |

    Application of an electrical field to Geobacter sulfurreducens biofilms stimulates production of OmcZ nanowires, which undergo a pH-induced conformational switch that causes increased stiffness and conductivity due to enhanced heme group π-stacking.

    • Sibel Ebru Yalcin
    • , J. Patrick O’Brien
    • , Yangqi Gu
    • , Krystle Reiss
    • , Sophia M. Yi
    • , Ruchi Jain
    • , Vishok Srikanth
    • , Peter J. Dahl
    • , Winston Huynh
    • , Dennis Vu
    • , Atanu Acharya
    • , Subhajyoti Chaudhuri
    • , Tamas Varga
    • , Victor S. Batista
    •  & Nikhil S. Malvankar
  • Article |

    An αHER2 antibody–neuraminidase conjugate, which selectively targets the removal of sialic acids from glycans on breast cancer cells, bypasses a glycoimmune checkpoint and enhances tumor cell killing by the host immune system.

    • Melissa A. Gray
    • , Michal A. Stanczak
    • , Natália R. Mantuano
    • , Han Xiao
    • , Johan F. A. Pijnenborg
    • , Stacy A. Malaker
    • , Caitlyn L. Miller
    • , Payton A. Weidenbacher
    • , Julia T. Tanzo
    • , Green Ahn
    • , Elliot C. Woods
    • , Heinz Läubli
    •  & Carolyn R. Bertozzi
  • Article |

    Among the adhesion receptor class of GPCRs, which are understudied, the adhesion receptor ADGRL3 can be activated by its own tethered agonist and couples to G protein G12/13 and somewhat to Gq.

    • Signe Mathiasen
    • , Tiago Palmisano
    • , Nicole A. Perry
    • , Hannah M. Stoveken
    • , Alex Vizurraga
    • , Dyke P. McEwen
    • , Najeah Okashah
    • , Tobias Langenhan
    • , Asuka Inoue
    • , Nevin A. Lambert
    • , Gregory G. Tall
    •  & Jonathan A. Javitch
  • Article |

    A PROTAC termed P4B targeting BRAF V600E mutant has been developed, which displays enhanced inhibitory function in cell lines carrying BRAF mutations that impart resistance to conventional BRAF inhibitors.

    • Ganna Posternak
    • , Xiaojing Tang
    • , Pierre Maisonneuve
    • , Ting Jin
    • , Hugo Lavoie
    • , Salima Daou
    • , Stephen Orlicky
    • , Theo Goullet de Rugy
    • , Lauren Caldwell
    • , Kin Chan
    • , Ahmed Aman
    • , Michael Prakesch
    • , Gennady Poda
    • , Pavel Mader
    • , Cassandra Wong
    • , Stefan Maier
    • , Julia Kitaygorodsky
    • , Brett Larsen
    • , Karen Colwill
    • , Zhe Yin
    • , Derek F. Ceccarelli
    • , Robert A. Batey
    • , Mikko Taipale
    • , Igor Kurinov
    • , David Uehling
    • , Jeff Wrana
    • , Daniel Durocher
    • , Anne-Claude Gingras
    • , Rima Al-Awar
    • , Marc Therrien
    •  & Frank Sicheri
  • Article |

    Genetic screens reveal a compendium of metabolic modifiers of lipid peroxidation. Tetrahydrobiopterin is essential under GPX4 inhibition, acting as a radical-trapping antioxidant that inhibits lipid peroxidation and is regenerated by DHFR.

    • Mariluz Soula
    • , Ross A. Weber
    • , Omkar Zilka
    • , Hanan Alwaseem
    • , Konnor La
    • , Frederick Yen
    • , Henrik Molina
    • , Javier Garcia-Bermudez
    • , Derek A. Pratt
    •  & Kıvanç Birsoy
  • Article |

    The fluorescent chemogenetic reporters greenFAST and redFAST were engineered by protein engineering. They display orthogonal fluorogen recognition and spectral properties allowing efficient multicolor imaging of proteins in live cells and organisms.

    • Alison G. Tebo
    • , Benjamien Moeyaert
    • , Marion Thauvin
    • , Irene Carlon-Andres
    • , Dorothea Böken
    • , Michel Volovitch
    • , Sergi Padilla-Parra
    • , Peter Dedecker
    • , Sophie Vriz
    •  & Arnaud Gautier
  • Article |

    The combination of heavy isotope labeling and ultra-high-pressure liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC–MS) is used to quantify modified genomic cytosines in pluripotent stem cells in different states and reveals active turnover of methylcytidine in oxidation-dependent and oxidation-independent manners.

    • Fabio Spada
    • , Sarah Schiffers
    • , Angie Kirchner
    • , Yingqian Zhang
    • , Gautier Arista
    • , Olesea Kosmatchev
    • , Eva Korytiakova
    • , René Rahimoff
    • , Charlotte Ebert
    •  & Thomas Carell
  • Article |

    Levels of the endogenous bile acid cholic acid-7-sulfate (CA7S) increase in the gastrointestinal tract of both mice and humans after sleeve gastrectomy. CA7S acts through the G-protein-coupled receptor TGR5 to increase glucose tolerance during insulin resistance.

    • Snehal N. Chaudhari
    • , David A. Harris
    • , Hassan Aliakbarian
    • , James N. Luo
    • , Matthew T. Henke
    • , Renuka Subramaniam
    • , Ashley H. Vernon
    • , Ali Tavakkoli
    • , Eric G. Sheu
    •  & A. Sloan Devlin
  • Article |

    Chemical profiling in hyponeddylated cells coupled with multi-omics target deconvolution led to the identification of molecular glue degraders of cyclin K that function by inducing proximity between the CRL adaptor DDB1 and a CDK12–cyclin K complex.

    • Cristina Mayor-Ruiz
    • , Sophie Bauer
    • , Matthias Brand
    • , Zuzanna Kozicka
    • , Marton Siklos
    • , Hana Imrichova
    • , Ines H. Kaltheuner
    • , Elisa Hahn
    • , Kristina Seiler
    • , Anna Koren
    • , Georg Petzold
    • , Michaela Fellner
    • , Christoph Bock
    • , André C. Müller
    • , Johannes Zuber
    • , Matthias Geyer
    • , Nicolas H. Thomä
    • , Stefan Kubicek
    •  & Georg E. Winter
  • Article |

    The structural analysis of a covalent E2–E3 ubiquitin transfer intermediate reveals the ubiquitin relay mechanism via two Cys residues of E3 ligase MYCBP2, which is related to neurite growth and neurite protection phenotypes.

    • Peter D. Mabbitt
    • , Andrea Loreto
    • , Marc-André Déry
    • , Adam J. Fletcher
    • , Mathew Stanley
    • , Kuan-Chuan Pao
    • , Nicola T. Wood
    • , Michael P. Coleman
    •  & Satpal Virdee
  • Article |

    An orthogonal O-glycan biosynthesis system was engineered in Escherichia coli to support the production of glycoproteins displaying human mucin O-glycans, including Tn antigens, in living bacteria and in cell-free extracts.

    • Aravind Natarajan
    • , Thapakorn Jaroentomeechai
    • , Marielisa Cabrera-Sánchez
    • , Jody C. Mohammed
    • , Emily C. Cox
    • , Olivia Young
    • , Asif Shajahan
    • , Michael Vilkhovoy
    • , Sandra Vadhin
    • , Jeffrey D. Varner
    • , Parastoo Azadi
    •  & Matthew P. DeLisa
  • Article |

    TAp63α monitors the genome integrity in oocytes. After DNA damage, TAp63α is activated, involving multiple phosphorylation steps by CK1 with different kinetics due to an unusual CK1/TAp63α interaction in which the product of one step inhibits the next.

    • Jakob Gebel
    • , Marcel Tuppi
    • , Apirat Chaikuad
    • , Katharina Hötte
    • , Martin Schröder
    • , Laura Schulz
    • , Frank Löhr
    • , Niklas Gutfreund
    • , Franziska Finke
    • , Erik Henrich
    • , Julija Mezhyrova
    • , Ralf Lehnert
    • , Francesco Pampaloni
    • , Gerhard Hummer
    • , Ernst H. K. Stelzer
    • , Stefan Knapp
    •  & Volker Dötsch
  • Article |

    Biocatalytic cascade reactions using engineered variants of ferulic acid decarboxylase coupled to carboxylic acid reductase utilize carbon dioxide fixation to enable the carboxylation and functionalization of styrene and other aromatic compounds.

    • Godwin A. Aleku
    • , Annica Saaret
    • , Ruth T. Bradshaw-Allen
    • , Sasha R. Derrington
    • , Gabriel R. Titchiner
    • , Irina Gostimskaya
    • , Deepankar Gahloth
    • , David A. Parker
    • , Sam Hay
    •  & David Leys
  • Article |

    A robust mass spectrometry workflow was developed for rapid drug perturbation profiling in multiple cell lines, enabling improved mechanistic deconvolution of single compounds and revealing novel mechanisms of action.

    • Benjamin Ruprecht
    • , Julie Di Bernardo
    • , Zhao Wang
    • , Xuan Mo
    • , Oleg Ursu
    • , Matthew Christopher
    • , Rafael B. Fernandez
    • , Li Zheng
    • , Brian D. Dill
    • , Huijun Wang
    • , Yuting Xu
    • , Andy Liaw
    • , Jonathan D. Mortison
    • , Nirodhini Siriwardana
    • , Brian Andresen
    • , Meir Glick
    • , James R. Tata
    • , Victoria Kutilek
    • , Ivan Cornella-Taracido
    •  & An Chi
  • Article |

    Highly selective activators of IRE1/XBP1 splicing pathway were identified via high-throughput screen, which promoted the degradation of APP and reduced APP-associated mitochondrial toxicity in an IRE1-dependent manner.

    • Julia M. D. Grandjean
    • , Aparajita Madhavan
    • , Lauren Cech
    • , Bryan O. Seguinot
    • , Ryan J. Paxman
    • , Emery Smith
    • , Louis Scampavia
    • , Evan T. Powers
    • , Christina B. Cooley
    • , Lars Plate
    • , Timothy P. Spicer
    • , Jeffery W. Kelly
    •  & R. Luke Wiseman