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Advances in fluorescence labeling strategies for dynamic cellular imaging

Nature Chemical Biology volume 10, pages 512–523 (2014)Cite this article

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Abstract

Synergistic advances in optical physics, probe design, molecular biology, labeling techniques and computational analysis have propelled fluorescence imaging into new realms of spatiotemporal resolution and sensitivity. This review aims to discuss advances in fluorescent probes and live-cell labeling strategies, two areas that remain pivotal for future advances in imaging technology. Fluorescent protein– and bio-orthogonal–based methods for protein and RNA imaging are discussed as well as emerging bioengineering techniques that enable their expression at specific genomic loci (for example, CRISPR and TALENs). Important attributes that contribute to the success of each technique are emphasized, providing a guideline for future advances in dynamic live-cell imaging.

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Figure 1: Spectral properties of chromophore classes found in FPs.
Figure 2: Covalent bio-orthogonal labeling mechanisms.
Figure 3: Bio-orthogonal labeling mechanisms based on reversible binding equilibria.
Figure 4: Methods for labeling RNA biomolecules.

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  1. Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, USA

    Kevin M Dean & Amy E Palmer

  2. BioFrontiers Institute, University of Colorado, Boulder, Colorado, USA

    Kevin M Dean & Amy E Palmer

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Dean, K., Palmer, A. Advances in fluorescence labeling strategies for dynamic cellular imaging. Nat Chem Biol 10, 512–523 (2014). https://doi.org/10.1038/nchembio.1556

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