Brief Communications

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  • A metabolic labeling method reveals that genomic N6-methyl-deoxyadenosine in mammalian cell lines originates not from direct methylation in DNA, but from a misincorporation of the metabolite of ribo-N6-methyladenosine.

    • Michael U. Musheev
    • Anne Baumgärtner
    • Christof Niehrs
    Brief Communication
  • The authors used an expanded genetic code to incorporate sulfated tyrosine into specific sites of proteins expressed in E. coli and mammalian cells and showed that sulfation of tyrosine at different sites had different functions.

    • James S. Italia
    • Jennifer C. Peeler
    • Abhishek Chatterjee
    Brief Communication
  • In depsipeptide synthetases, intact adenylation and ketoreductase domains responsible for selecting and reducing α-keto acids are flanked by two halves of a split pseudo-Asub domain whose physical interaction is critical for the module's activity.

    • Diego A. Alonzo
    • Clarisse Chiche-Lapierre
    • T. Martin Schmeing
    Brief Communication
  • The authors designed a chemical probe, azido-kethoxal, to specifically label guanosine in single-strand RNAs in live cells that could be used to determine transcriptome-wide RNA secondary structures.

    • Xiaocheng Weng
    • Jing Gong
    • Chuan He
    Brief Communication
  • The topology of homodimeric membrane protein EmrE is dynamic and includes unassisted flipping of an N-terminal helix in and out of the membrane long after co-translational insertion. Dimerization locks the helix to limit topological dynamics.

    • Maximilian Seurig
    • Moira Ek
    • Nir Fluman
    Brief Communication
  • An inhibitor of the complement pathway of the innate immune system targets the human complement component 5 protein (C5) by binding to an interfacial pocket to prevent its proteolytic cleavage by the last enzyme of the complement pathway, C5 convertase.

    • Keith Jendza
    • Mitsunori Kato
    • Gregory A. Michaud
    Brief Communication
  • MCC950, a small-molecule inhibitor of the NLRP3 inflammasome, inactivates NLRP3, including hyperactive disease-linked mutations, by closing the ‘open’ conformation, thereby preventing conformational changes required for NLRP3 activation.

    • Ana Tapia-Abellán
    • Diego Angosto-Bazarra
    • Pablo Pelegrin
    Brief Communication
  • Engineering of toehold-gated guide RNA (thgRNA) by tethering toehold riboswitches to sgRNAs enables the activation of CRISPR–Cas9 genome editing or transcriptional regulation in response to complementary synthetic or endogenous cellular RNAs.

    • Ka-Hei Siu
    • Wilfred Chen
    Brief Communication
  • Structural analysis of prostaglandin E receptor EP3, a member of the prostanoid receptor subfamily of GPCRs, in complex with the endogenous agonist PGE2 reveals important interactions and motions required for receptor activation.

    • Kazushi Morimoto
    • Ryoji Suno
    • Takuya Kobayashi
    Brief Communication
  • Plant-associated rhizosphere bacteria produce gramibactin, a cyclic lipodepsipeptide siderophore that tightly binds iron via an unexpected functional group, the N-nitrosohydroxylamine (diazeniumdiolate) moieties of the amino acid graminine.

    • Ron Hermenau
    • Keishi Ishida
    • Christian Hertweck
    Brief Communication