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  • Only one protein factor is known that senses Na+ and controls gene expression. The Breaker Laboratory describes a bacterial riboswitch class selective for Na+ that regulates genes important for Na+ homeostasis, pH maintenance, osmotic stress response and ATP synthesis.

    • Neil White
    • Harini Sadeeshkumar
    • Ronald R. Breaker
    Article Open Access
  • Biomolecular condensates form and dissolve in response to a wide range of signals. A new study reports a solubility-based phosphoproteome-profiling approach, which uncovers the extensive role of phosphorylation in regulating protein partitioning into condensates across the human proteome.

    • Kamran Rizzolo
    • Diana M. Mitrea
    News & Views
  • Chemical probes are powerful tools for the discovery of ligand-binding and drug-target sites on proteins. A new software helps to profile their performance in chemoproteomic applications.

    • Alexander Leitner
    News & Views
  • Mutant-selective KRAS-targeting drugs hold great promise for the treatment of some of the most aggressive forms of cancer. Building on the breakthrough success of KRAS-G12C inhibitors, researchers have now found a way to target another mutant KRAS with serine-modifying covalent inhibitors.

    • Micah J. Niphakis
    • Benjamin F. Cravatt
    News & Views
  • A directed evolution approach delivers ribosomes with highly functional tethered subunits. Combining the decoding and peptidyl transferase activities of the ribosome into a single entity sets the scene for more efficient protein engineering technologies.

    • Aleksandra Filipovska
    • Oliver Rackham
    News & Views
  • Using single-molecule magnetic tweezers and biochemical methods, Naqvi et al. revealed how CRISPR–Cas12a regulates the DNA cleavage rate through a conserved stacking interaction between the R-loop and the W355 residue and the sequence of the CRISPR RNA 3′ end.

    • Mohsin M. Naqvi
    • Laura Lee
    • Mark D. Szczelkun
    Article Open Access
  • The de novo design of a pair of complementary peptides, one basic for cell penetration and target binding and one acidic that can be fused to proteins of interest, provides an approach for delivery into mammalian cells and subcellular targeting.

    • Guto G. Rhys
    • Jessica A. Cross
    • Derek N. Woolfson
  • A combination of engineering a fluorine-selective trans-acyltransferase and manipulation of the fluorinated extender unit pool in Escherichia coli enables the production of site-selectively fluorinated erythromycin precursors in vitro in vitroin vitro and in vivo.

    • Sasilada Sirirungruang
    • Omer Ad
    • Michelle C. Y. Chang
  • A combined screening and selection approach enables the evolution of the generalist transcription factor RamR into specific and sensitive biosensors for various alkaloids and in turn a streamlined pathway for tetrahydropapaverine biosynthesis.

    • Simon d’Oelsnitz
    • Wantae Kim
    • Andrew D. Ellington
  • Functional screening of a large metagenomic library with a droplet microfluidics platform enabled the discovery of SN243, a bacterial β-glucuronidase from the glycoside hydrolase 3 family, which was characterized structurally and biochemically.

    • Stefanie Neun
    • Paul Brear
    • Florian Hollfelder
  • The Hippo pathway is a key regulator of tissue homeostasis, organ size and cancer. Identification of microcolin B and its analog molecules as Hippo pathway activators connects PtdIns4P-dependent lipid signaling with the Hippo pathway, suggesting potential targets for cancer therapy.

    • Gayoung Seo
    • Wenqi Wang
    News & Views