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  • Transposon-associated transposase B (TnpB) is the putative ancestor of Cas nucleases. A TnpB-based adenine base editor has now been developed that is small enough to be loaded into a single AAV vector without compromising editing activity.

    • Beomjong Song
    • Sangsu Bae
    News & Views
  • A hypercompact adenine base editor termed TaRGET-ABE was developed by fusing a catalytically inactive transposon-associated transposase B guided by an engineered RNA to deaminases, which achieves efficient A-to-G conversions via adeno-associated viral delivery in mammalian genomes.

    • Do Yon Kim
    • Yuhee Chung
    • Yong-Sam Kim
    Article
  • Emerging evidence shows that ubiquitination can occur on nonlysine residues and nonproteinaceous substrates. This perspective summarizes the recent discoveries in noncanonical ubiquitination and advocates the development of chemical biology tools for new substrate identification and mechanistic dissection.

    • Daniel R. Squair
    • Satpal Virdee
    Perspective
  • In the post-genomic era, many genes and regulatory elements remain uncharacterized, including riboswitches — RNA structures that control gene expression by directly binding metabolites. Here, a new type of metal-responsive riboswitch that senses sodium cations expands the bacterial Na+ metabolic repertoire.

    • Lauren Waters
    News & Views
  • Only one protein factor is known that senses Na+ and controls gene expression. The Breaker Laboratory describes a bacterial riboswitch class selective for Na+ that regulates genes important for Na+ homeostasis, pH maintenance, osmotic stress response and ATP synthesis.

    • Neil White
    • Harini Sadeeshkumar
    • Ronald R. Breaker
    Article Open Access
  • Biomolecular condensates form and dissolve in response to a wide range of signals. A new study reports a solubility-based phosphoproteome-profiling approach, which uncovers the extensive role of phosphorylation in regulating protein partitioning into condensates across the human proteome.

    • Kamran Rizzolo
    • Diana M. Mitrea
    News & Views
  • Chemical probes are powerful tools for the discovery of ligand-binding and drug-target sites on proteins. A new software helps to profile their performance in chemoproteomic applications.

    • Alexander Leitner
    News & Views
  • Mutant-selective KRAS-targeting drugs hold great promise for the treatment of some of the most aggressive forms of cancer. Building on the breakthrough success of KRAS-G12C inhibitors, researchers have now found a way to target another mutant KRAS with serine-modifying covalent inhibitors.

    • Micah J. Niphakis
    • Benjamin F. Cravatt
    News & Views