Volume 17 Issue 8, August 2015

Volume 17 Issue 8

Inhibition of NF-κB signalling or the downstream factor DOTL1 in aged fibroblasts increases their reprogramming efficiency.p1004

Review

News and Views

  • News & Views |

    Mitotic chromosome condensation has fascinated biologists since Flemming's early illustrations of mitosis in the late nineteenth century. Now — 130 years later — chromatid condensation is reconstituted in vitro with the minimum components. The results are remarkably and beautifully simple, requiring only core histones, three histone chaperones, topoisomerase II and condensin I.

    • Jason C. Bell
    •  & Aaron F. Straight
  • News & Views |

    Modelling organs in culture has great potential to improve our understanding of development, organogenesis and disease. While some endodermal derived organs have been modelled, the corpus region of the stomach, where acid-producing cells reside, remained an invincible target. A 60-day differentiation protocol now enables the generation of functional acid-producing cells in culture, conquering the challenge.

    • Meritxell Huch
  • News & Views |

    Activator E2Fs and Myc cooperate as master regulators of proliferation. A new study sheds light on one of the fundamental questions in cancer biology: how do oncogenic changes, such as Retinoblastoma (RB)-mutation, modify E2F and Myc activity?

    • Wayne O. Miles
    •  & Nicholas J. Dyson

Articles

  • Article |

    Forbes and colleagues report on a population of hepatic progenitor cells that regenerate the adult liver in a mouse model where more than 98% of all hepatocytes are irreversibly damaged.

    • Wei-Yu Lu
    • , Thomas G. Bird
    • , Luke Boulter
    • , Atsunori Tsuchiya
    • , Alicia M. Cole
    • , Trevor Hay
    • , Rachel V. Guest
    • , Davina Wojtacha
    • , Tak Yung Man
    • , Alison Mackinnon
    • , Rachel A. Ridgway
    • , Timothy Kendall
    • , Michael J. Williams
    • , Thomas Jamieson
    • , Alex Raven
    • , David C. Hay
    • , John P. Iredale
    • , Alan R. Clarke
    • , Owen J. Sansom
    •  & Stuart J. Forbes
  • Article |

    Noguchi and colleagues report the generation of stomach-like tissue from mouse embryonic stem cells. They show that the tissue contains all stomach-specific cell types and secretes acid and digestive enzyme.

    • Taka-aki K. Noguchi
    • , Naoto Ninomiya
    • , Mari Sekine
    • , Shinji Komazaki
    • , Pi-Chao Wang
    • , Makoto Asashima
    •  & Akira Kurisaki
  • Article |

    Cowan and colleagues report a method to generate mature endothelial or vascular smooth muscle cells from human pluripotent stem cells with high efficiency and purity.

    • Christoph Patsch
    • , Ludivine Challet-Meylan
    • , Eva C. Thoma
    • , Eduard Urich
    • , Tobias Heckel
    • , John F. O’Sullivan
    • , Stephanie J. Grainger
    • , Friedrich G. Kapp
    • , Lin Sun
    • , Klaus Christensen
    • , Yulei Xia
    • , Mary H. C. Florido
    • , Wei He
    • , Wei Pan
    • , Michael Prummer
    • , Curtis R. Warren
    • , Roland Jakob-Roetne
    • , Ulrich Certa
    • , Ravi Jagasia
    • , Per-Ola Freskgård
    • , Isaac Adatto
    • , Dorothee Kling
    • , Paul Huang
    • , Leonard I. Zon
    • , Elliot L. Chaikof
    • , Robert E. Gerszten
    • , Martin Graf
    • , Roberto Iacone
    •  & Chad A. Cowan
  • Article |

    Lopez-Otin and colleagues report that NF-κB signalling hyperactivation in progeria syndrome fibroblasts inhibits their reprogramming into iPS cells. Blockade of this pathway or the downstream factor DOTL1 rescues senescence-related phenotypes.

    • Clara Soria-Valles
    • , Fernando G. Osorio
    • , Ana Gutiérrez-Fernández
    • , Alejandro De Los Angeles
    • , Clara Bueno
    • , Pablo Menéndez
    • , José I. Martín-Subero
    • , George Q. Daley
    • , José M. P. Freije
    •  & Carlos López-Otín
  • Article |

    Leone and colleagues find that in intestinal crypt cells, Myc and E2F transcription factors control the transition into the G2 phase of the cell cycle, but in the absence of Rb, Myc and E2f3a drive S phase entry and ectopic proliferation in vivo.

    • Huayang Liu
    • , Xing Tang
    • , Arunima Srivastava
    • , Thierry Pécot
    • , Piotr Daniel
    • , Benjamin Hemmelgarn
    • , Stephan Reyes
    • , Nicholas Fackler
    • , Amneet Bajwa
    • , Raleigh Kladney
    • , Christopher Koivisto
    • , Zhong Chen
    • , Qianben Wang
    • , Kun Huang
    • , Raghu Machiraju
    • , Maria Teresa Sáenz-Robles
    • , Paul Cantalupo
    • , James M. Pipas
    •  & Gustavo Leone
  • Article |

    Campisi and colleagues show that the MTOR inhibitor rapamycin blocks the senescence-associated secretory phenotype (SASP) by inhibiting translation of IL1A, which prevents senescent fibroblasts from promoting tumour growth.

    • Remi-Martin Laberge
    • , Yu Sun
    • , Arturo V. Orjalo
    • , Christopher K. Patil
    • , Adam Freund
    • , Lili Zhou
    • , Samuel C. Curran
    • , Albert R. Davalos
    • , Kathleen A. Wilson-Edell
    • , Su Liu
    • , Chandani Limbad
    • , Marco Demaria
    • , Patrick Li
    • , Gene B. Hubbard
    • , Yuji Ikeno
    • , Martin Javors
    • , Pierre-Yves Desprez
    • , Christopher C. Benz
    • , Pankaj Kapahi
    • , Peter S. Nelson
    •  & Judith Campisi
  • Article |

    Romagnolo and colleagues report that Atg7 deficiency has a tumour-suppressive role in the intestinal epithelium, by inducing a microbiome-influenced immune response and inhibiting tumour growth through p53- and AMPK-related mechanisms.

    • Jonathan Lévy
    • , Wulfran Cacheux
    • , Medhi Ait Bara
    • , Antoine L’Hermitte
    • , Patricia Lepage
    • , Marie Fraudeau
    • , Coralie Trentesaux
    • , Julie Lemarchand
    • , Aurélie Durand
    • , Anne-Marie Crain
    • , Carmen Marchiol
    • , Gilles Renault
    • , Florent Dumont
    • , Franck Letourneur
    • , Myriam Delacre
    • , Alain Schmitt
    • , Benoit Terris
    • , Christine Perret
    • , Mathias Chamaillard
    • , Jean-Pierre Couty
    •  & Béatrice Romagnolo

Resource

  • Resource |

    In an effort by several research groups, a genome-wide RNAi-based screen presents a list of validated genes involved in cilia assembly and maintenance, and reveals previously unidentified ciliopathy factors.

    • Gabrielle Wheway
    • , Miriam Schmidts
    • , Dorus A. Mans
    • , Katarzyna Szymanska
    • , Thanh-Minh T. Nguyen
    • , Hilary Racher
    • , Ian G. Phelps
    • , Grischa Toedt
    • , Julie Kennedy
    • , Kirsten A. Wunderlich
    • , Nasrin Sorusch
    • , Zakia A. Abdelhamed
    • , Subaashini Natarajan
    • , Warren Herridge
    • , Jeroen van Reeuwijk
    • , Nicola Horn
    • , Karsten Boldt
    • , David A. Parry
    • , Stef J. F. Letteboer
    • , Susanne Roosing
    • , Matthew Adams
    • , Sandra M. Bell
    • , Jacquelyn Bond
    • , Julie Higgins
    • , Ewan E. Morrison
    • , Darren C. Tomlinson
    • , Gisela G. Slaats
    • , Teunis J. P. van Dam
    • , Lijia Huang
    • , Kristin Kessler
    • , Andreas Giessl
    • , Clare V. Logan
    • , Evan A. Boyle
    • , Jay Shendure
    • , Shamsa Anazi
    • , Mohammed Aldahmesh
    • , Selwa Al Hazzaa
    • , Robert A. Hegele
    • , Carole Ober
    • , Patrick Frosk
    • , Aizeddin A. Mhanni
    • , Bernard N. Chodirker
    • , Albert E. Chudley
    • , Ryan Lamont
    • , Francois P. Bernier
    • , Chandree L. Beaulieu
    • , Paul Gordon
    • , Richard T. Pon
    • , Clem Donahue
    • , A. James Barkovich
    • , Louis Wolf
    • , Carmel Toomes
    • , Christian T. Thiel
    • , Kym M. Boycott
    • , Martin McKibbin
    • , Chris F. Inglehearn
    • , UK10K Consortium
    • , University of Washington Center for Mendelian Genomics
    • , Fiona Stewart
    • , Heymut Omran
    • , Martijn A. Huynen
    • , Panagiotis I. Sergouniotis
    • , Fowzan S. Alkuraya
    • , Jillian S. Parboosingh
    • , A. Micheil Innes
    • , Colin E. Willoughby
    • , Rachel H. Giles
    • , Andrew R. Webster
    • , Marius Ueffing
    • , Oliver Blacque
    • , Joseph G. Gleeson
    • , Uwe Wolfrum
    • , Philip L. Beales
    • , Toby Gibson
    • , Dan Doherty
    • , Hannah M. Mitchison
    • , Ronald Roepman
    •  & Colin A. Johnson

Corrigendum

Erratum