Volume 17 Issue 7, July 2015

Volume 17 Issue 7

Microtubule initiation from a template is slow but the probability of initiation is increased by proteins that regulate microtubule dynamics.

[article p907]

Review

Obituary

  • Obituary |

    • Filippo G. Giancotti
    • , Cole M. Haynes
    • , Laura M. Machesky
    • , Michael F. Olson
    •  & Michael Overholtzer

News and Views

  • News & Views |

    The eukaryotic cell uses two complex machineries to degrade unwanted proteins. The first is the ubiquitin–proteasome system and the second is autophagy. A new study contributes to our understanding of how the two systems interconnect to coordinate protein degradation.

    • Sascha Martens
    •  & Andreas Bachmair
  • News & Views |

    Phagocytic cells engulf their prey into vesicular structures called phagosomes, of which a certain proportion becomes demarcated for enhanced maturation by a process called LC3-associated phagocytosis (LAP). Light has now been shed on the molecular requirements of LAP, establishing a central role for the protein Rubicon in the immune response to Aspergillus fumigatus.

    • Keith B. Boyle
    •  & Felix Randow
  • News & Views |

    Integrin-based focal adhesions integrate biochemical and biomechanical signals from the extracellular matrix and the actin cytoskeleton. The combination of three-dimensional super-resolution imaging and loss- or gain-of-function protein mutants now links the nanoscale dynamic localization of proteins to their activation and function within focal adhesions.

    • Grégory Giannone

Articles

  • Article |

    By measuring surface tensions in developing mouse embryos, Maître and colleagues show that compaction of the blastomere stage embryo is driven by downregulation of actomyosin at cell–cell contacts.

    • Jean-Léon Maître
    • , Ritsuya Niwayama
    • , Hervé Turlier
    • , François Nédélec
    •  & Takashi Hiiragi
  • Article |

    Pei and colleagues report that the oncogene c-Jun acts as a somatic cell reprogramming barrier, and that its truncated dominant-negative form can substitute for Oct4 in reprogramming.

    • Jing Liu
    • , Qingkai Han
    • , Tianran Peng
    • , Meixiu Peng
    • , Bei Wei
    • , Dongwei Li
    • , Xiaoshan Wang
    • , Shengyong Yu
    • , Jiaqi Yang
    • , Shangtao Cao
    • , Kaimeng Huang
    • , Andrew Paul Hutchins
    • , He Liu
    • , Junqi Kuang
    • , Zhiwei Zhou
    • , Jing Chen
    • , Haoyu Wu
    • , Lin Guo
    • , Yongqiang Chen
    • , You Chen
    • , Xuejia Li
    • , Hongling Wu
    • , Baojian Liao
    • , Wei He
    • , Hong Song
    • , Hongjie Yao
    • , Guangjin Pan
    • , Jiekai Chen
    •  & Duanqing Pei
  • Article |

    Waterman and colleagues use super-resolution microscopy and biosensor technology to characterize the spatiotemporal regulation of the protein interactions within focal adhesions that control vinculin activation and function during focal adhesion maturation.

    • Lindsay B. Case
    • , Michelle A. Baird
    • , Gleb Shtengel
    • , Sharon L. Campbell
    • , Harald F. Hess
    • , Michael W. Davidson
    •  & Clare M. Waterman
  • Article |

    Green and colleagues characterize LC3-associated phagocytosis as a process that depends on Rubicon, Beclin-1, UVRAG and VPS34 but not on canonical autophagy proteins.

    • Jennifer Martinez
    • , R. K. Subbarao Malireddi
    • , Qun Lu
    • , Larissa Dias Cunha
    • , Stephane Pelletier
    • , Sebastien Gingras
    • , Robert Orchard
    • , Jun-Lin Guan
    • , Haiyan Tan
    • , Junmin Peng
    • , Thirumala-Devi Kanneganti
    • , Herbert W. Virgin
    •  & Douglas R. Green
  • Article |

    Kim and colleagues and Kwon and colleagues reveal that amino-terminal arginylation of BiP promotes its targeting to autophagy adaptor p62 and subsequent lysosomal degradation of BiP, p62 and associated cargo.

    • Hyunjoo Cha-Molstad
    • , Ki Sa Sung
    • , Joonsung Hwang
    • , Kyoung A. Kim
    • , Ji Eun Yu
    • , Young Dong Yoo
    • , Jun Min Jang
    • , Dong Hoon Han
    • , Michael Molstad
    • , Jung Gi Kim
    • , Yoon Jee Lee
    • , Adriana Zakrzewska
    • , Su-Hyeon Kim
    • , Sung Tae Kim
    • , Sun Yong Kim
    • , Hee Gu Lee
    • , Nak Kyun Soung
    • , Jong Seog Ahn
    • , Aaron Ciechanover
    • , Bo Yeon Kim
    •  & Yong Tae Kwon
  • Article |

    Williamson and colleagues demonstrate that mRNA-decapping enzyme Dcp2 is phosphorylated by mTORC1, leading to degradation of Atg mRNA and suppression of autophagy, influencing immunity and inflammation.

    • Guowu Hu
    • , Travis McQuiston
    • , Amélie Bernard
    • , Yoon-Dong Park
    • , Jin Qiu
    • , Ali Vural
    • , Nannan Zhang
    • , Scott R. Waterman
    • , Nathan H. Blewett
    • , Timothy G. Myers
    • , Richard J. Maraia
    • , John H. Kehrl
    • , Gulbu Uzel
    • , Daniel J. Klionsky
    •  & Peter R. Williamson
  • Article |

    Tavazoie and colleagues report that activation of pannexin-1 channels and the subsequent stimulation of autocrine purinergic signalling promotes the survival of disseminated cancer cells in the microvasculature of metastasis target organs.

    • Paul W. Furlow
    • , Steven Zhang
    • , T. David Soong
    • , Nils Halberg
    • , Hani Goodarzi
    • , Creed Mangrum
    • , Y. Gloria Wu
    • , Olivier Elemento
    •  & Sohail F. Tavazoie

Corrigenda