Volume 17 Issue 12, December 2015

Volume 17 Issue 12

Systems-level analysis of the integrin adhesome to define the core cell adhesion machinery.p1577

Editorials

  • Editorial |

    In the mission to reduce irreproducibility, true change can only come about if all stakeholders — researchers, institutions, funders and journals — join together with common purpose.

News and Views

  • News & Views |

    Tumours reprogram their metabolism to maximize macromolecule biosynthesis for growth. However, which of the common tumour-associated metabolic activities are critical for proliferation remains unclear. Glutamate-derived glutamine is now shown to satisfy the glutamine needs of glioblastoma, indicating that glutamine anaplerosis is dispensable for growth.

    • Abigail S. Krall
    •  & Heather R. Christofk
  • News & Views |

    A powerful combination of two-colour imaging in vivo, Fourier-filtered kymography and simulations provides a high-resolution view of kinesin-2 transport dynamics in cilia. This study reveals heterotrimeric kinesin-II as an 'obstacle-course runner' and homodimeric OSM-3 (KIF17) as a 'long-distance runner', and elucidates the 'baton handoff' between these two kinesin-2 motors on the microtubule track.

    • Robert O'Hagan
    •  & Maureen M. Barr
  • News & Views |

    Spatiotemporally distinct pluripotent states captured in vitro provide an accessible way of modelling early human development. An intricate interplay between the metabolome and histone modifications is now shown to drive the metabolic switch from human naive to primed pluripotency, one of the earliest steps of embryogenesis.

    • Jun Wu
    •  & Juan Carlos Izpisua Belmonte

Articles

  • Article |

    By comparing the metabolomes, transcriptomes and epigenomes of human pluripotent stem cell lines, Sperber et al. show that interplay between the metabolome and histone modifications drives the metabolic switch from naive to primed pluripotency.

    • Henrik Sperber
    • , Julie Mathieu
    • , Yuliang Wang
    • , Amy Ferreccio
    • , Jennifer Hesson
    • , Zhuojin Xu
    • , Karin A. Fischer
    • , Arikketh Devi
    • , Damien Detraux
    • , Haiwei Gu
    • , Stephanie L. Battle
    • , Megan Showalter
    • , Cristina Valensisi
    • , Jason H. Bielas
    • , Nolan G. Ericson
    • , Lilyana Margaretha
    • , Aaron M. Robitaille
    • , Daciana Margineantu
    • , Oliver Fiehn
    • , David Hockenbery
    • , C. Anthony Blau
    • , Daniel Raftery
    • , Adam A. Margolin
    • , R. David Hawkins
    • , Randall T. Moon
    • , Carol B. Ware
    •  & Hannele Ruohola-Baker
  • Article |

    Through a proteomics approach, Qi and colleagues and Long and colleagues identify the sensor of the unfolded protein response IRE1α as an endogenous substrate of the E3 ubiquitin ligase involved in ER-associated degradation, Hrd1.

    • Shengyi Sun
    • , Guojun Shi
    • , Haibo Sha
    • , Yewei Ji
    • , Xuemei Han
    • , Xin Shu
    • , Hongming Ma
    • , Takamasa Inoue
    • , Beixue Gao
    • , Hana Kim
    • , Pengcheng Bu
    • , Robert D. Guber
    • , Xiling Shen
    • , Ann-Hwee Lee
    • , Takao Iwawaki
    • , Adrienne W. Paton
    • , James C. Paton
    • , Deyu Fang
    • , Billy Tsai
    • , John R. Yates III
    • , Haoquan Wu
    • , Sander Kersten
    • , Qiaoming Long
    • , Gerald E. Duhamel
    • , Kenneth W. Simpson
    •  & Ling Qi
  • Article |

    Gottlieb and colleagues demonstrate that glioblastoma cell proliferation under glutamine starvation conditions depends on the glutamine-synthetase-dependent conversion of glutamate to glutamine to fuel purine biosynthesis and cell growth.

    • Saverio Tardito
    • , Anaïs Oudin
    • , Shafiq U. Ahmed
    • , Fred Fack
    • , Olivier Keunen
    • , Liang Zheng
    • , Hrvoje Miletic
    • , Per Øystein Sakariassen
    • , Adam Weinstock
    • , Allon Wagner
    • , Susan L. Lindsay
    • , Andreas K. Hock
    • , Susan C. Barnett
    • , Eytan Ruppin
    • , Svein Harald Mørkve
    • , Morten Lund-Johansen
    • , Anthony J. Chalmers
    • , Rolf Bjerkvig
    • , Simone P. Niclou
    •  & Eyal Gottlieb

Letter

Resource

  • Resource |

    Humphries and colleagues analyse proteomic data of integrin adhesion complexes to derive a consensus integrin adhesome and characterize the temporal dynamics of adhesome component recruitment during adhesion complex assembly and disassembly.

    • Edward R. Horton
    • , Adam Byron
    • , Janet A. Askari
    • , Daniel H. J. Ng
    • , Angélique Millon-Frémillon
    • , Joseph Robertson
    • , Ewa J. Koper
    • , Nikki R. Paul
    • , Stacey Warwood
    • , David Knight
    • , Jonathan D. Humphries
    •  & Martin J. Humphries

Technical Reports

  • Technical Report |

    Austen et al. generated talin biosensors to study integrin-based force transduction. They report that extracellular rigidity sensing requires talin’s mechanical engagement and find talin isoform-dependent effects in integrin-mediated mechanosensing.

    • Katharina Austen
    • , Pia Ringer
    • , Alexander Mehlich
    • , Anna Chrostek-Grashoff
    • , Carleen Kluger
    • , Christoph Klingner
    • , Benedikt Sabass
    • , Roy Zent
    • , Matthias Rief
    •  & Carsten Grashoff

Addendum

Addendum