Volume 17 Issue 11, November 2015

Volume 17 Issue 11

Competition for actin between two distinct F-actin networks determines whether epithelial cells remain stationary or migrate.

[article p1435]

News and Views

  • News & Views |

    Protein ubiquitylation in mammals is known to trigger selective autophagy of peroxisomes through a process termed pexophagy. The physiological peroxisomal target for pexophagy-related ubiquitylation has been controversial, but two studies have now identified the protein PEX5 as the real candidate.

    • Suresh Subramani
  • News & Views |

    The mechanisms underlying integrin-dependent signalling are a topic of continued study. Endocytosed integrins are now shown to drive assembly of signalling complexes on the cytoplasmic face of endocytic membranes to promote cancer cell survival and increase metastatic capacity following cell detachment.

    • Elena Rainero
    •  & Jim C. Norman
  • News & Views |

    Two studies show that the E3 ubiquitin ligase RNF138 is recruited to DNA double-strand break sites, where it ubiquitylates key repair factors to promote DNA-end resection and homologous recombination. These findings add insights into the multilayered regulatory mechanisms underlying DNA double-strand break repair pathway choice in mammalian cells.

    • Simon Bekker-Jensen
    •  & Niels Mailand

Articles

  • Article |

    Ding and colleagues show that somatic cell reprogramming does not depend on Atg5-dependent canonical autophagy, but requires mitochondrial clearance in an Atg5-independent manner downstream of AMPK.

    • Tianhua Ma
    • , Jun Li
    • , Yue Xu
    • , Chen Yu
    • , Tao Xu
    • , Haixia Wang
    • , Kai Liu
    • , Nan Cao
    • , Bao-ming Nie
    • , Sai-yong Zhu
    • , Shaohua Xu
    • , Ke Li
    • , Wan-guo Wei
    • , Yuzhang Wu
    • , Kun-liang Guan
    •  & Sheng Ding
  • Article |

    Huynh and colleagues discover nuclear rotations driven by centrosomes, microtubules and Dynein in Drosophila germ cells, and find that these movements facilitate the pairing of homologous chromosomes.

    • Nicolas Christophorou
    • , Thomas Rubin
    • , Isabelle Bonnet
    • , Tristan Piolot
    • , Marion Arnaud
    •  & Jean-René Huynh
  • Article |

    Ivaska and colleagues report that endocytosed integrins are able to signal from endosomes in an FAK-dependent manner. They further show that endosomal integrin signalling can promote anoikis resistance and lung colonization in cancer cells.

    • Jonna Alanko
    • , Anja Mai
    • , Guillaume Jacquemet
    • , Kristine Schauer
    • , Riina Kaukonen
    • , Markku Saari
    • , Bruno Goud
    •  & Johanna Ivaska
  • Article |

    Lomakin et al. report that the competition for actin between two distinct F-actin networks determines whether epithelial cells remain stationary or migrate, with myosin II inhibiting the migratory polarized phenotype by confining actin in contractile bundles.

    • Alexis J. Lomakin
    • , Kun-Chun Lee
    • , Sangyoon J. Han
    • , Duyen A. Bui
    • , Michael Davidson
    • , Alex Mogilner
    •  & Gaudenz Danuser
  • Article |

    Jackson and colleagues and Hendzel and colleagues reveal that the E3 ligase RNF138 functions in the repair of double-strand breaks by promoting CtIP accumulation and displacement of DNA-PK subunit Ku.

    • Ismail Hassan Ismail
    • , Jean-Philippe Gagné
    • , Marie-Michelle Genois
    • , Hilmar Strickfaden
    • , Darin McDonald
    • , Zhizhong Xu
    • , Guy G. Poirier
    • , Jean-Yves Masson
    •  & Michael J. Hendzel
  • Article |

    Jackson and colleagues and Hendzel and colleagues reveal that the E3 ligase RNF138 functions in the repair of double-strand breaks by promoting CtIP accumulation and displacement of DNA-PK subunit Ku.

    • Christine K. Schmidt
    • , Yaron Galanty
    • , Matylda Sczaniecka-Clift
    • , Julia Coates
    • , Satpal Jhujh
    • , Mukerrem Demir
    • , Matthew Cornwell
    • , Petra Beli
    •  & Stephen P. Jackson
  • Article |

    Using live imaging of Xenopus and starfish oocytes and embryos undergoing cytokinesis, Bement and colleagues show that anaphase onset promotes cortical waves of Rho and F-actin, which can be modelled by reaction–diffusion dynamics.

    • William M. Bement
    • , Marcin Leda
    • , Alison M. Moe
    • , Angela M. Kita
    • , Matthew E. Larson
    • , Adriana E. Golding
    • , Courtney Pfeuti
    • , Kuan-Chung Su
    • , Ann L. Miller
    • , Andrew B. Goryachev
    •  & George von Dassow
  • Article |

    Chen and colleagues report that the third enzyme in the oxidative pentose phosphate pathway (PPP), 6PGD, controls cancer cell proliferation by regulating LKB1–AMPK signalling. Inhibitors of 6PGD decrease tumorigenesis in mouse xenografts.

    • Ruiting Lin
    • , Shannon Elf
    • , Changliang Shan
    • , Hee-Bum Kang
    • , Quanjiang Ji
    • , Lu Zhou
    • , Taro Hitosugi
    • , Liang Zhang
    • , Shuai Zhang
    • , Jae Ho Seo
    • , Jianxin Xie
    • , Meghan Tucker
    • , Ting-Lei Gu
    • , Jessica Sudderth
    • , Lei Jiang
    • , Matthew Mitsche
    • , Ralph J. DeBerardinis
    • , Shaoxiong Wu
    • , Yuancheng Li
    • , Hui Mao
    • , Peng R. Chen
    • , Dongsheng Wang
    • , Georgia Zhuo Chen
    • , Selwyn J. Hurwitz
    • , Sagar Lonial
    • , Martha L. Arellano
    • , Hanna J. Khoury
    • , Fadlo R. Khuri
    • , Benjamin H. Lee
    • , Qunying Lei
    • , Daniel J. Brat
    • , Keqiang Ye
    • , Titus J. Boggon
    • , Chuan He
    • , Sumin Kang
    • , Jun Fan
    •  & Jing Chen

Letters

Corrigendum