Resources

Xu et al. provide a single-cell transcriptomic atlas of 4–6 week human embryos, thereby profiling early human organogenesis.

Sountoulidis et al. provide a spatial gene expression atlas of human embryonic lung during the first trimester of gestation and identify 83 cell identities corresponding to stable cell types or transitional states.

Using single-cell analysis, Tan et al. map the cellular and spatial hierarchy and heterogeneity of eutopic endometrium and characterize ectopic peritoneal and ovarian endometriosis lesions from individuals receiving hormone treatment.

Using an optimized Ribo-seq protocol that is applicable for low-input samples, Xie, Li and colleagues revealed the translation landscape during oocyte-to-embryo transition and in pre-implantation embryos.

Abe et al. profile, characterize and compare non-haematopoietic cells in normal human lymph nodes versus nodal lymphomas from patients, providing insights into stromal modelling in health and disease.

Jijumon et al. develop a medium-throughput, lysate-based approach to characterize microtubule interactors, starting here with a set of 45 proteins, and describe unique microtubule behaviours and microtubule-associated activities.

Wang et al. analysed post-mortem samples of the lungs of patients with COVID-19 by bulk and single-nucleus RNA sequencing along with proteomics and discovered lung senescence as a feature of COVID-19 pathology.

Constructing a single-cell transcriptional map of primary human epidermal melanocytes, Belote et al. uncover distinct subpopulations of melanocytes, characterize dedifferentiation patterns associated with melanoma prognosis and uncover the unique cellular origins of acral melanoma.

You et al. report single-cell ATAC-seq profiles of periphery immune cells from patients recovered from COVID-19, which reveals a global remodelling of the chromatin accessibility that may contribute to immune memory formation.

Using an unbiased and quantitative proteomic approach, Kugeratski et al. identified the core constituents of exosomes and found that syntenin-1 was the most abundant component, making it a putative universal biomarker.

Saichi et al. performed single-cell RNA-seq analysis of antigen-presenting cells (APCs) isolated from the peripheral blood of patients with moderate and severe COVID-19 and uncovered defects in antiviral immune response in specific APC subsets.

Shi et al. profiled small non-coding RNAs (sncRNAs) through PANDORA-seq, which identified tissue-specific transfer RNA- and ribosomal RNA-derived small RNAs, as well as sncRNAs, with dynamic changes during induced pluripotent stem cell reprogramming.

Pijuan-Sala et al. present a comprehensive single-nucleus open chromatin map of early mouse embryogenesis and validate the role of ETS transcription factor FEV in endothelial identity in zebrafish.

Müller et al. provide a comprehensive resource depicting cellular substrates, localization and interacting partners of RhoGEF and RhoGAP proteins regulating the canonical Rho family of GTPases.

Wang, Yu, Zhou, Song et al. profile cardiomyocytes and neighbouring cells from healthy adults and patients with heart failure and in recovery, and delineate their cellular compositions and interaction networks.

Using a BioID approach, Bagci et al. systematically analyse the Rho-family GTPase interactome and reveal previously unappreciated interactions with RhoGEFs and RhoGAPs and effectors for Rho proteins.

Brumbaugh, Kim et al. generate mice with inducible H3 K-to-M mutations, which globally inhibit methylation at specific sites, and analyse specific phenotypes and changes in chromatin accessibility and transcriptional programmes.

Xia and colleagues provided a map for transcriptome-wide distribution of m⁶A RNA methylation in eight human fetal tissue types.

Using a multi-tier scRNA-seq and CRISP-seq approach, Giladi et al. define a transcriptional signature for the naive haematopoietic stem cell state, and follow progenitor plasticity and fate commitment under the influence of cytokines and growth factors.

Using scCOOL-seq, Li et al. simultaneously characterize the DNA methylation and chromatin accessibility of the same cell during human preimplantation development.