Resources

Liu, Neve et al. use large-scale loss-of-function RNA-interference screens to identify circular RNAs that are direct regulators of important signalling pathways and also common essential and tissue-specific circRNAs.

In two independent studies, Sun, Liu et al. and Sun et al. develop SPATAC-seq to map the chromatin accessibility landscape of zebrafish embryogenesis and mouse organogenesis, respectively, and identify transcription regulators that determine cell fate.

In two independent studies, Sun, Liu et al. and Sun et al. develop SPATAC-seq to map the chromatin accessibility landscape of zebrafish embryogenesis and mouse organogenesis, respectively, and identify transcription regulators that determine cell fate.

Chidley et al. report a CRISPR interference/activation screening platform to systematically interrogate the contribution of nutrient transporters to support cancer cell proliferation in environments of variable composition.

Morgan, Pernes and colleagues perform mass spectrometry-based targeted lipidomics and provide a comprehensive lipid profile of human and mouse immune cells, which they then show confer differential ferroptosis susceptibilities.

Roider, Baertsch et al. present a spatially resolved resource map of the T cell infiltration landscape across and within patients with distinct B cell non-Hodgkin lymphoma entities.

Zwick et al. examine longitudinal transcriptional patterns across the entire mouse and human small intestine and find that, in both species, the small intestine comprises five domains of nutrient absorption and three regional stem cell populations.

Using low-input lipidomics in mouse and human embryos, Zhang, Shui, Li and colleagues find that lipid unsaturation increases with development towards the blastocyst stage. They further show that lipid desaturases contribute to successful embryo implantation.

Lin Li, Lei Li et al. perform single-cell multi-omics to study the transcriptome, the DNA methylome and chromatin accessibility in human arrested embryos and find that cytoskeletal defects cause embryonic arrest characterized by zygotic genome activation.

Huang, Li, An, Yang, Cui et al. perform a single-cell multi-omics analysis of human spermatogenesis and identify a DNA demethylation event upon meiosis initiation, which is associated with meiotic recombination.

Eom, Peak, Park, Ahn and colleagues reveal and provide a comprehensive resource of 8-oxoguanine modifications in tumour microRNAs and show how they differentially influence malignancy progression in gliomas and hepatocellular carcinoma.

Ton, Keitley et al. provide a morphological and molecular atlas of rabbit development. Comparative studies reveal that combining rabbit and mouse atlases can serve as a model for dissecting early primate development.

Augsornworawat et al. perform single-nucleus multi-omics and integrated transcriptional and chromatin analysis to identify differences between human stem cell-derived and primary islets.

Xu et al. provide a single-cell transcriptomic atlas of 4–6 week human embryos, thereby profiling early human organogenesis.

Sountoulidis et al. provide a spatial gene expression atlas of human embryonic lung during the first trimester of gestation and identify 83 cell identities corresponding to stable cell types or transitional states.

Using single-cell analysis, Tan et al. map the cellular and spatial hierarchy and heterogeneity of eutopic endometrium and characterize ectopic peritoneal and ovarian endometriosis lesions from individuals receiving hormone treatment.

Using an optimized Ribo-seq protocol that is applicable for low-input samples, Xie, Li and colleagues revealed the translation landscape during oocyte-to-embryo transition and in pre-implantation embryos.

Abe et al. profile, characterize and compare non-haematopoietic cells in normal human lymph nodes versus nodal lymphomas from patients, providing insights into stromal modelling in health and disease.

Jijumon et al. develop a medium-throughput, lysate-based approach to characterize microtubule interactors, starting here with a set of 45 proteins, and describe unique microtubule behaviours and microtubule-associated activities.

Wang et al. analysed post-mortem samples of the lungs of patients with COVID-19 by bulk and single-nucleus RNA sequencing along with proteomics and discovered lung senescence as a feature of COVID-19 pathology.