Letters

  • Letter |

    Xu et al. report that nuclear SIRT1 is recognized as an autophagy substrate during senescence and also observe ageing of the immune system.

    • Caiyue Xu
    • , Lu Wang
    • , Parinaz Fozouni
    • , Gry Evjen
    • , Vemika Chandra
    • , Jing Jiang
    • , Congcong Lu
    • , Michael Nicastri
    • , Corey Bretz
    • , Jeffrey D. Winkler
    • , Ravi Amaravadi
    • , Benjamin A. Garcia
    • , Peter D. Adams
    • , Melanie Ott
    • , Wei Tong
    • , Terje Johansen
    • , Zhixun Dou
    •  & Shelley L. Berger
  • Letter |

    Phase separation concentrates mitochondrial RNA granules. Here Rey et al., show that mitochondrial RNA granules (MRGs) behaviour is consistent with liquid–liquid phase separation (LLPS) and their fusion coincides with mitochondrial remodelling.

    • Timo Rey
    • , Sofia Zaganelli
    • , Emilie Cuillery
    • , Evangelia Vartholomaiou
    • , Marie Croisier
    • , Jean-Claude Martinou
    •  & Suliana Manley
  • Letter |

    Xu, Liu, Pen, Zhang et al. demonstrate that the SEL1L–HRD1 complex, which is part of the ERAD protein quality control machinery, safeguards haematopoietic stem cell identity and niche location by ensuring the haematopoietic stem cell surface expression of MPL.

    • Longyong Xu
    • , Xia Liu
    • , Fanglue Peng
    • , Weijie Zhang
    • , Liting Zheng
    • , Yao Ding
    • , Tianpeng Gu
    • , Kaosheng Lv
    • , Jin Wang
    • , Laura Ortinau
    • , Tianyuan Hu
    • , Xiangguo Shi
    • , Guojun Shi
    • , Ge Shang
    • , Shengyi Sun
    • , Takao Iwawaki
    • , Yewei Ji
    • , Wei Li
    • , Jeffrey M. Rosen
    • , Xiang H.-F. Zhang
    • , Dongsu Park
    • , Stanley Adoro
    • , Andre Catic
    • , Wei Tong
    • , Ling Qi
    • , Daisuke Nakada
    •  & Xi Chen
  • Letter |

    Wei et al. show that clusters of unphosphorylated RNA polymerase II seed the nucleation of phase-separated condensates of TAF15, which further recruit RNA polymerase II to amplify transcriptional activation.

    • Ming-Tzo Wei
    • , Yi-Che Chang
    • , Shunsuke F. Shimobayashi
    • , Yongdae Shin
    • , Amy R. Strom
    •  & Clifford P. Brangwynne
  • Letter |

    Two complementary studies from the laboratories of Riegman et al. and Katikaneni et al., respectively, identify a key role for controlled wave-like propagation of lipid peroxide signalling during wound detection in vivo and in ferroptotic cell death.

    • Michelle Riegman
    • , Liran Sagie
    • , Chen Galed
    • , Tom Levin
    • , Noah Steinberg
    • , Scott J. Dixon
    • , Ulrich Wiesner
    • , Michelle S. Bradbury
    • , Philipp Niethammer
    • , Assaf Zaritsky
    •  & Michael Overholtzer
  • Letter |

    Two complementary studies from the laboratories of Riegman et al. and Katikaneni et al., respectively, identify a key role for controlled wave-like propagation of lipid peroxide signalling during wound detection in vivo, and in ferroptotic cell death.

    • Anushka Katikaneni
    • , Mark Jelcic
    • , Gary F. Gerlach
    • , Yanan Ma
    • , Michael Overholtzer
    •  & Philipp Niethammer
  • Letter |

    Liu et al. characterize a mechanism of UFL1-mediated UFMylation of p53 at multiple lysine residues and show how this dampens MDM2-induced ubiquitination of p53 to enhance its stability and tumour-suppressive function.

    • Jiang Liu
    • , Di Guan
    • , Maogong Dong
    • , Jingjing Yang
    • , Haibin Wei
    • , Qian Liang
    • , Lizhi Song
    • , Lu Xu
    • , Junjie Bai
    • , Cui Liu
    • , Jian Mao
    • , Qian Zhang
    • , Junzhi Zhou
    • , Xiaoying Wu
    • , Miao Wang
    •  & Yu-Sheng Cong
  • Letter |

    Sato et al. demonstrate that IRF2, which negatively regulates interferon signalling, safeguards intestinal stem cells against non-infectious, sterile interferon stress by limiting their differentiation into secretory lineages.

    • Taku Sato
    • , Shun Ishikawa
    • , Jumpei Asano
    • , Hirona Yamamoto
    • , Masayuki Fujii
    • , Toshiro Sato
    • , Kouhei Yamamoto
    • , Keisuke Kitagaki
    • , Takumi Akashi
    • , Ryuichi Okamoto
    •  & Toshiaki Ohteki
  • Letter |

    Hsu et al. find that the protein kinase Akt acts as a co-adaptor in the clathrin coat complex and is needed in conjunction with ACAP1 to bind to cargo proteins to promote their recycling.

    • Jia-Wei Hsu
    • , Ming Bai
    • , Kunhua Li
    • , Jia-Shu Yang
    • , Nam Chu
    • , Philip A. Cole
    • , Michael J. Eck
    • , Jian Li
    •  & Victor W. Hsu
  • Letter |

    Tumour fibroblasts influence oncolytic viral therapy. Arwert et al. show that transcytosis of cancer cells into fibroblasts activates STING and IRF3 to upregulate interferon-β1, eliciting a transcriptional program to reduce the effectiveness of oncolytic viral therapy.

    • Esther N. Arwert
    • , Emma L. Milford
    • , Antonio Rullan
    • , Stefanie Derzsi
    • , Steven Hooper
    • , Takuya Kato
    • , David Mansfield
    • , Alan Melcher
    • , Kevin J. Harrington
    •  & Erik Sahai
  • Letter |

    Borg et al. report that incorporation of the sperm-specific histone variant H3.10, which resists K27 methylation, causes H3K27me3 removal from sperm chromatin in plants, thus facilitating the expression of sperm-specific genes.

    • Michael Borg
    • , Yannick Jacob
    • , Daichi Susaki
    • , Chantal LeBlanc
    • , Daniel Buendía
    • , Elin Axelsson
    • , Tomokazu Kawashima
    • , Philipp Voigt
    • , Leonor Boavida
    • , Jörg Becker
    • , Tetsuya Higashiyama
    • , Robert Martienssen
    •  & Frédéric Berger
  • Letter |

    Three independent but complementary studies by the laboratories of Markus, Reck-Peterson and Yildiz identify a key role for LIS1 in modulating localization, activity and function of dynein in cells.

    • Zaw Min Htet
    • , John P. Gillies
    • , Richard W. Baker
    • , Andres E. Leschziner
    • , Morgan E. DeSantis
    •  & Samara L. Reck-Peterson
  • Letter |

    Martinez-Hoyer et al. identify recurrent TP53 or RUNX1 variants in patients with lenalidomide-resistant myelodysplastic syndromes that are associated with impaired RUNX1/GATA2-mediated megakaryocytic differentiation and cell death.

    • Sergio Martinez-Høyer
    • , Yu Deng
    • , Jeremy Parker
    • , Jihong Jiang
    • , Angela Mo
    • , T. Roderick Docking
    • , Nadia Gharaee
    • , Jenny Li
    • , Patricia Umlandt
    • , Megan Fuller
    • , Martin Jädersten
    • , Austin Kulasekararaj
    • , Luca Malcovati
    • , Alan F. List
    • , Eva Hellström-Lindberg
    • , Uwe Platzbecker
    •  & Aly Karsan
  • Letter |

    Hoffmann and colleagues report that the mammalian maternal-to-zygotic transition requires KDM4A-mediated removal of H3K9me3 from the broad H3K4me3 domains in oocytes.

    • Aditya Sankar
    • , Mads Lerdrup
    • , Adeel Manaf
    • , Jens Vilstrup Johansen
    • , Javier Martin Gonzalez
    • , Rehannah Borup
    • , Robert Blanshard
    • , Arne Klungland
    • , Klaus Hansen
    • , Claus Yding Andersen
    • , John Arne Dahl
    • , Kristian Helin
    •  & Eva R. Hoffmann
  • Letter |

    Santoriello, Sporrij et al. show that the progesterone receptor associates with RNA helicase DDX21 during nucleotide depletion, promotes its binding on chromatin and rescues efficient transcription in melanoma cells.

    • Cristina Santoriello
    • , Audrey Sporrij
    • , Song Yang
    • , Ryan A. Flynn
    • , Telmo Henriques
    • , Bilguujin Dorjsuren
    • , Eugenia Custo Greig
    • , Wyatt McCall
    • , Meredith E. Stanhope
    • , Maurizio Fazio
    • , Michael Superdock
    • , Asher Lichtig
    • , Isaac Adatto
    • , Brian J. Abraham
    • , Marian Kalocsay
    • , Michael Jurynec
    • , Yi Zhou
    • , Karen Adelman
    • , Eliezer Calo
    •  & Leonard I. Zon
  • Letter |

    Montagner et al. show that lung epithelial cells induce Sfrp2 expression and fibronectin fibril formation in disseminated breast cancer cells, thereby promoting their survival and latency in the lung.

    • Marco Montagner
    • , Rahul Bhome
    • , Steven Hooper
    • , Probir Chakravarty
    • , Xiao Qin
    • , Jahangir Sufi
    • , Ajay Bhargava
    • , Colin D. H. Ratcliffe
    • , Yutaka Naito
    • , Arianna Pocaterra
    • , Christopher J. Tape
    •  & Erik Sahai
  • Letter |

    Li et al. demonstrate the efficacy of correcting the mutated TERT promoter using a programmable base editing, highlighting its ability to induce senescence, arrest and regression in brain tumour models.

    • Xinjian Li
    • , Xu Qian
    • , Bin Wang
    • , Yan Xia
    • , Yanhua Zheng
    • , Linyong Du
    • , Daqian Xu
    • , Dongming Xing
    • , Ronald A. DePinho
    •  & Zhimin Lu
  • Letter |

    Lau et al. quantify endogenous concentrations of the chemokine Cxcl12 and its binding affinity for its cognate receptor Cxcr4 in zebrafish embryos, uncovering a negative-feedback loop governing directional cell migration in vivo.

    • Stephanie Lau
    • , Anna Feitzinger
    • , Gayatri Venkiteswaran
    • , John Wang
    • , Stephen W. Lewellis
    • , Chad A. Koplinski
    • , Francis C. Peterson
    • , Brian F. Volkman
    • , Martin Meier-Schellersheim
    •  & Holger Knaut
  • Letter |

    Lee et al. show that, after nitrogen starvation and genetic interference with the architecture of nuclear pore complexes, nucleoporins are degraded by autophagy, constituting a quality-control step at the nuclear envelope.

    • Chia-Wei Lee
    • , Florian Wilfling
    • , Paolo Ronchi
    • , Matteo Allegretti
    • , Shyamal Mosalaganti
    • , Stefan Jentsch
    • , Martin Beck
    •  & Boris Pfander
  • Letter |

    Li et al. show that BCAA transaminase 2 enhances uptake and catabolism of branched-chain amino acids, thereby promoting development of pancreatic ductal adenocarcinoma harbouring KRAS mutations.

    • Jin-Tao Li
    • , Miao Yin
    • , Di Wang
    • , Jian Wang
    • , Ming-Zhu Lei
    • , Ye Zhang
    • , Ying Liu
    • , Lei Zhang
    • , Shao-Wu Zou
    • , Li-Peng Hu
    • , Zhi-Gang Zhang
    • , Yi-Ping Wang
    • , Wen-Yu Wen
    • , Hao-Jie Lu
    • , Zheng-Jun Chen
    • , Dan Su
    •  & Qun-Ying Lei
  • Letter |

    Gaglia et al. show, using single-cell imaging and analysis in human tumours, that phase transition of heat-shock factor 1 (HSF1) to form intranuclear stress bodies mediates cell-fate decisions underlying cell survival or death.

    • Giorgio Gaglia
    • , Rumana Rashid
    • , Clarence Yapp
    • , Gaurav N. Joshi
    • , Carmen G. Li
    • , Susan L. Lindquist
    • , Kristopher A. Sarosiek
    • , Luke Whitesell
    • , Peter K. Sorger
    •  & Sandro Santagata
  • Letter |

    Zhang et al. show that ketogenesis-derived β-hydroxybutyrate (BHB) epigenetically modifies H3K9 of Foxo1 and Ppargc1a to regulate Pck1, which in turn controls metabolic flux and CD8+ memory T-cell development.

    • Huafeng Zhang
    • , Ke Tang
    • , Jingwei Ma
    • , Li Zhou
    • , Jincheng Liu
    • , Liping Zeng
    • , Liyan Zhu
    • , Pingwei Xu
    • , Jie Chen
    • , Keke Wei
    • , Xiaoyu Liang
    • , Jiadi Lv
    • , Jing Xie
    • , Yuying Liu
    • , Yonghong Wan
    •  & Bo Huang
  • Letter |

    Tan et al. and Siahaan et al. present distinct but complementary data showing that microtubule regulates dynamic condensation of tau molecules, and this in turn affects microtubule biology and function.

    • Ruensern Tan
    • , Aileen J. Lam
    • , Tracy Tan
    • , Jisoo Han
    • , Dan W. Nowakowski
    • , Michael Vershinin
    • , Sergi Simó
    • , Kassandra M. Ori-McKenney
    •  & Richard J. McKenney
  • Letter |

    Using intravital imaging, Ebrahim et al. show that actin and non-muscle myosin II assemble into polyhedral lattices around the vesicle membrane to mediate exocytic secretion in live tissues.

    • Seham Ebrahim
    • , Desu Chen
    • , Max Weiss
    • , Lenka Malec
    • , Yeap Ng
    • , Ivan Rebustini
    • , Evan Krystofiak
    • , Longhua Hu
    • , Jian Liu
    • , Andrius Masedunskas
    • , Edna Hardeman
    • , Peter Gunning
    • , Bechara Kachar
    •  & Roberto Weigert
  • Letter |

    Andersen, Hannezo, Ulyanchenko et al. map cell behaviour and spatiotemporal dynamics of the sebaceous gland during homeostasis and oncogene-induced gland expansion, and show that all basal cells contribute to long-term gland maintenance.

    • Marianne Stemann Andersen
    • , Edouard Hannezo
    • , Svetlana Ulyanchenko
    • , Soline Estrach
    • , Yasuko Antoku
    • , Sabrina Pisano
    • , Kim E. Boonekamp
    • , Sarah Sendrup
    • , Martti Maimets
    • , Marianne Terndrup Pedersen
    • , Jens V. Johansen
    • , Ditte L. Clement
    • , Chloe C. Feral
    • , Benjamin D. Simons
    •  & Kim B. Jensen
  • Letter |

    Nakahara et al. identify five transcriptional regulators that can revitalize Nestin-expressing mesenchymal stromal cells to enhance the synthesis of haematopoietic stem cell niche factors, improve haematopoietic stem cell expansion and protect them against DNA damage.

    • Fumio Nakahara
    • , Daniel K. Borger
    • , Qiaozhi Wei
    • , Sandra Pinho
    • , Maria Maryanovich
    • , Ali H. Zahalka
    • , Masako Suzuki
    • , Cristian D. Cruz
    • , Zichen Wang
    • , Chunliang Xu
    • , Philip E. Boulais
    • , Avi Ma’ayan
    • , John M. Greally
    •  & Paul S. Frenette
  • Letter |

    Nakamura et al. show that DNA repair pathway choice and initiation of homologous recombination is guided by the recruitment of BRCA1 to post-replicative chromatin by BARD1 recognition of histone H4 tails unmethylated on lysine 20.

    • Kyosuke Nakamura
    • , Giulia Saredi
    • , Jordan R. Becker
    • , Benjamin M. Foster
    • , Nhuong V. Nguyen
    • , Tracey E. Beyer
    • , Laura C. Cesa
    • , Peter A. Faull
    • , Saulius Lukauskas
    • , Thomas Frimurer
    • , J. Ross Chapman
    • , Till Bartke
    •  & Anja Groth
  • Letter |

    Kraft et al. show that chromosomal inversions that relocate a limb enhancer can establish asymmetric stripes of the enhancer with downstream genes, resulting in ectopic gene expression and limb phenotypes.

    • Katerina Kraft
    • , Andreas Magg
    • , Verena Heinrich
    • , Christina Riemenschneider
    • , Robert Schöpflin
    • , Julia Markowski
    • , Daniel M. Ibrahim
    • , Rocío Acuna-Hidalgo
    • , Alexandra Despang
    • , Guillaume Andrey
    • , Lars Wittler
    • , Bernd Timmermann
    • , Martin Vingron
    •  & Stefan Mundlos
  • Letter |

    By using multicolour single-molecule live imaging, Moon et al. show that the dynamics of the interaction between mRNAs and ribonucleoprotein granules are affected by translational status, mRNA length and granule size.

    • Stephanie L. Moon
    • , Tatsuya Morisaki
    • , Anthony Khong
    • , Kenneth Lyon
    • , Roy Parker
    •  & Timothy J. Stasevich
  • Letter |

    Xin et al. track stem cells in live mice to show that fate determination is a flexible process during homeostatic hair follicle growth, and progenitor cells can change differentiation outcomes as a consequence of dynamic relocation.

    • Tianchi Xin
    • , David Gonzalez
    • , Panteleimon Rompolas
    •  & Valentina Greco
  • Letter |

    di Ronza et al. identify CLN8 as a cargo receptor for lysosomal enzymes required for their endoplasmic-reticulum-to-Golgi transport, linking Batten disease caused by CLN8 mutations to defects in organelle biogenesis.

    • Alberto di Ronza
    • , Lakshya Bajaj
    • , Jaiprakash Sharma
    • , Deepthi Sanagasetti
    • , Parisa Lotfi
    • , Carolyn Joy Adamski
    • , John Collette
    • , Michela Palmieri
    • , Abdallah Amawi
    • , Lauren Popp
    • , Kevin Tommy Chang
    • , Maria Chiara Meschini
    • , Hon-Chiu Eastwood Leung
    • , Laura Segatori
    • , Alessandro Simonati
    • , Richard Norman Sifers
    • , Filippo Maria Santorelli
    •  & Marco Sardiello
  • Letter |

    Using super-resolution microscopy, tracking of single particle trajectories of endoplasmic reticulum (ER) luminal proteins traversing tubular ER, and measuring ER dynamics, Holcman et al. show that ER content is propelled by active luminal flow.

    • David Holcman
    • , Pierre Parutto
    • , Joseph E. Chambers
    • , Marcus Fantham
    • , Laurence J. Young
    • , Stefan J. Marciniak
    • , Clemens F. Kaminski
    • , David Ron
    •  & Edward Avezov
  • Letter |

    Studying early Drosophila wing epithelium development, Singh et al. find that individual cells are mechanically autonomous, and that their elongation along the proximal–distal axis is driven by polarized microtubule-mediated pushing forces.

    • Amrita Singh
    • , Tanumoy Saha
    • , Isabell Begemann
    • , Andrea Ricker
    • , Harald Nüsse
    • , Oliver Thorn-Seshold
    • , Jürgen Klingauf
    • , Milos Galic
    •  & Maja Matis
  • Letter |

    Chan Wah Hak et al. show how plasma membrane patches are primed for fast endophilin-mediated endocytosis and disassembly, in the absence of receptor stimulation, through FBP17 and CIP4 binding to SHIP2 and lamellipodin.

    • Laura Chan Wah Hak
    • , Shaheen Khan
    • , Ilaria Di Meglio
    • , Ah-Lai Law
    • , Safa Lucken-Ardjomande Häsler
    • , Leonor M. Quintaneiro
    • , Antonio P. A. Ferreira
    • , Matthias Krause
    • , Harvey T. McMahon
    •  & Emmanuel Boucrot
  • Letter |

    Garcia-Bermudez et al. and Sullivan et al. show that endogenous aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours, and that metformin depletes aspartate to limit tumour growth.

    • Lucas B. Sullivan
    • , Alba Luengo
    • , Laura V. Danai
    • , Lauren N. Bush
    • , Frances F. Diehl
    • , Aaron M. Hosios
    • , Allison N. Lau
    • , Sarah Elmiligy
    • , Scott Malstrom
    • , Caroline A. Lewis
    •  & Matthew G. Vander Heiden
  • Letter |

    Garcia-Bermudez et al. and Sullivan et al. show that endogenous aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours, and that metformin depletes aspartate to limit tumour growth.

    • Javier Garcia-Bermudez
    • , Lou Baudrier
    • , Konnor La
    • , Xiphias Ge Zhu
    • , Justine Fidelin
    • , Vladislav O. Sviderskiy
    • , Thales Papagiannakopoulos
    • , Henrik Molina
    • , Matija Snuderl
    • , Caroline A. Lewis
    • , Richard L. Possemato
    •  & Kıvanç Birsoy
  • Letter |

    Monitoring growing epithelial cells through the cell cycle, Uroz et al. find that cell–cell tension and cell–matrix traction forces differ across the cell cycle and affect cell cycle duration, the G1–S transition and mitotic rounding.

    • Marina Uroz
    • , Sabrina Wistorf
    • , Xavier Serra-Picamal
    • , Vito Conte
    • , Marta Sales-Pardo
    • , Pere Roca-Cusachs
    • , Roger Guimerà
    •  & Xavier Trepat
  • Letter |

    Zhang et al. report that tRNA methyltransferase Dnmt2 is required for sperm small-non-coding-RNA-mediated transmission of paternal metabolic disorders to the offspring.

    • Yunfang Zhang
    • , Xudong Zhang
    • , Junchao Shi
    • , Francesca Tuorto
    • , Xin Li
    • , Yusheng Liu
    • , Reinhard Liebers
    • , Liwen Zhang
    • , Yongcun Qu
    • , Jingjing Qian
    • , Maya Pahima
    • , Ying Liu
    • , Menghong Yan
    • , Zhonghong Cao
    • , Xiaohua Lei
    • , Yujing Cao
    • , Hongying Peng
    • , Shichao Liu
    • , Yue Wang
    • , Huili Zheng
    • , Rebekah Woolsey
    • , David Quilici
    • , Qiwei Zhai
    • , Lei Li
    • , Tong Zhou
    • , Wei Yan
    • , Frank Lyko
    • , Ying Zhang
    • , Qi Zhou
    • , Enkui Duan
    •  & Qi Chen
  • Letter |

    Using super-resolution microscopy and cryo-electron microscopy, Stoldt et al. show that mitochondrial transcript translation and OXPHOS complex assembly are spatially partitioned within the mitochondrial membrane.

    • Stefan Stoldt
    • , Dirk Wenzel
    • , Kirsten Kehrein
    • , Dietmar Riedel
    • , Martin Ott
    •  & Stefan Jakobs
  • Letter |

    Floros et al. show that mtDNA copy number is reduced and non-synonymous mtDNA mutations are eliminated to prevent mtDNA mutation accumulation in germ cells during human primordial germ cell development.

    • Vasileios I. Floros
    • , Angela Pyle
    • , Sabine Dietmann
    • , Wei Wei
    • , Walfred C. W. Tang
    • , Naoko Irie
    • , Brendan Payne
    • , Antonio Capalbo
    • , Laila Noli
    • , Jonathan Coxhead
    • , Gavin Hudson
    • , Moira Crosier
    • , Henrik Strahl
    • , Yacoub Khalaf
    • , Mitinori Saitou
    • , Dusko Ilic
    • , M. Azim Surani
    •  & Patrick F. Chinnery
  • Letter |

    Ibarra-Soria et al. study cellular diversity, transcriptional signatures, lineage specification and somitogenesis on a single-cell level in E8.25 mouse embryos, and reveal the regulation of blood progenitor formation by the leukotriene pathway.

    • Ximena Ibarra-Soria
    • , Wajid Jawaid
    • , Blanca Pijuan-Sala
    • , Vasileios Ladopoulos
    • , Antonio Scialdone
    • , David J. Jörg
    • , Richard C. V. Tyser
    • , Fernando J. Calero-Nieto
    • , Carla Mulas
    • , Jennifer Nichols
    • , Ludovic Vallier
    • , Shankar Srinivas
    • , Benjamin D. Simons
    • , Berthold Göttgens
    •  & John C. Marioni
  • Letter |

    Ingallina et al. show that mutant p53 is protected from degradation in response to matrix stiffness in a manner dependent on RhoA geranylgeranylation and actomyosin dynamics.

    • Eleonora Ingallina
    • , Giovanni Sorrentino
    • , Rebecca Bertolio
    • , Kamil Lisek
    • , Alessandro Zannini
    • , Luca Azzolin
    • , Luisa Ulloa Severino
    • , Denis Scaini
    • , Miguel Mano
    • , Fiamma Mantovani
    • , Antonio Rosato
    • , Silvio Bicciato
    • , Stefano Piccolo
    •  & Giannino Del Sal