Letters

  • Letter |

    Using super-resolution microscopy, tracking of single particle trajectories of endoplasmic reticulum (ER) luminal proteins traversing tubular ER, and measuring ER dynamics, Holcman et al. show that ER content is propelled by active luminal flow.

    • David Holcman
    • , Pierre Parutto
    • , Joseph E. Chambers
    • , Marcus Fantham
    • , Laurence J. Young
    • , Stefan J. Marciniak
    • , Clemens F. Kaminski
    • , David Ron
    •  & Edward Avezov
  • Letter |

    Studying early Drosophila wing epithelium development, Singh et al. find that individual cells are mechanically autonomous, and that their elongation along the proximal–distal axis is driven by polarized microtubule-mediated pushing forces.

    • Amrita Singh
    • , Tanumoy Saha
    • , Isabell Begemann
    • , Andrea Ricker
    • , Harald Nüsse
    • , Oliver Thorn-Seshold
    • , Jürgen Klingauf
    • , Milos Galic
    •  & Maja Matis
  • Letter |

    Chan Wah Hak et al. show how plasma membrane patches are primed for fast endophilin-mediated endocytosis and disassembly, in the absence of receptor stimulation, through FBP17 and CIP4 binding to SHIP2 and lamellipodin.

    • Laura Chan Wah Hak
    • , Shaheen Khan
    • , Ilaria Di Meglio
    • , Ah-Lai Law
    • , Safa Lucken-Ardjomande Häsler
    • , Leonor M. Quintaneiro
    • , Antonio P. A. Ferreira
    • , Matthias Krause
    • , Harvey T. McMahon
    •  & Emmanuel Boucrot
  • Letter |

    Garcia-Bermudez et al. and Sullivan et al. show that endogenous aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours, and that metformin depletes aspartate to limit tumour growth.

    • Lucas B. Sullivan
    • , Alba Luengo
    • , Laura V. Danai
    • , Lauren N. Bush
    • , Frances F. Diehl
    • , Aaron M. Hosios
    • , Allison N. Lau
    • , Sarah Elmiligy
    • , Scott Malstrom
    • , Caroline A. Lewis
    •  & Matthew G. Vander Heiden
  • Letter |

    Garcia-Bermudez et al. and Sullivan et al. show that endogenous aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours, and that metformin depletes aspartate to limit tumour growth.

    • Javier Garcia-Bermudez
    • , Lou Baudrier
    • , Konnor La
    • , Xiphias Ge Zhu
    • , Justine Fidelin
    • , Vladislav O. Sviderskiy
    • , Thales Papagiannakopoulos
    • , Henrik Molina
    • , Matija Snuderl
    • , Caroline A. Lewis
    • , Richard L. Possemato
    •  & Kıvanç Birsoy
  • Letter |

    Monitoring growing epithelial cells through the cell cycle, Uroz et al. find that cell–cell tension and cell–matrix traction forces differ across the cell cycle and affect cell cycle duration, the G1–S transition and mitotic rounding.

    • Marina Uroz
    • , Sabrina Wistorf
    • , Xavier Serra-Picamal
    • , Vito Conte
    • , Marta Sales-Pardo
    • , Pere Roca-Cusachs
    • , Roger Guimerà
    •  & Xavier Trepat
  • Letter |

    Zhang et al. report that tRNA methyltransferase Dnmt2 is required for sperm small-non-coding-RNA-mediated transmission of paternal metabolic disorders to the offspring.

    • Yunfang Zhang
    • , Xudong Zhang
    • , Junchao Shi
    • , Francesca Tuorto
    • , Xin Li
    • , Yusheng Liu
    • , Reinhard Liebers
    • , Liwen Zhang
    • , Yongcun Qu
    • , Jingjing Qian
    • , Maya Pahima
    • , Ying Liu
    • , Menghong Yan
    • , Zhonghong Cao
    • , Xiaohua Lei
    • , Yujing Cao
    • , Hongying Peng
    • , Shichao Liu
    • , Yue Wang
    • , Huili Zheng
    • , Rebekah Woolsey
    • , David Quilici
    • , Qiwei Zhai
    • , Lei Li
    • , Tong Zhou
    • , Wei Yan
    • , Frank Lyko
    • , Ying Zhang
    • , Qi Zhou
    • , Enkui Duan
    •  & Qi Chen
  • Letter |

    Using super-resolution microscopy and cryo-electron microscopy, Stoldt et al. show that mitochondrial transcript translation and OXPHOS complex assembly are spatially partitioned within the mitochondrial membrane.

    • Stefan Stoldt
    • , Dirk Wenzel
    • , Kirsten Kehrein
    • , Dietmar Riedel
    • , Martin Ott
    •  & Stefan Jakobs
  • Letter |

    Floros et al. show that mtDNA copy number is reduced and non-synonymous mtDNA mutations are eliminated to prevent mtDNA mutation accumulation in germ cells during human primordial germ cell development.

    • Vasileios I. Floros
    • , Angela Pyle
    • , Sabine Dietmann
    • , Wei Wei
    • , Walfred C. W. Tang
    • , Naoko Irie
    • , Brendan Payne
    • , Antonio Capalbo
    • , Laila Noli
    • , Jonathan Coxhead
    • , Gavin Hudson
    • , Moira Crosier
    • , Henrik Strahl
    • , Yacoub Khalaf
    • , Mitinori Saitou
    • , Dusko Ilic
    • , M. Azim Surani
    •  & Patrick F. Chinnery
  • Letter |

    Ibarra-Soria et al. study cellular diversity, transcriptional signatures, lineage specification and somitogenesis on a single-cell level in E8.25 mouse embryos, and reveal the regulation of blood progenitor formation by the leukotriene pathway.

    • Ximena Ibarra-Soria
    • , Wajid Jawaid
    • , Blanca Pijuan-Sala
    • , Vasileios Ladopoulos
    • , Antonio Scialdone
    • , David J. Jörg
    • , Richard C. V. Tyser
    • , Fernando J. Calero-Nieto
    • , Carla Mulas
    • , Jennifer Nichols
    • , Ludovic Vallier
    • , Shankar Srinivas
    • , Benjamin D. Simons
    • , Berthold Göttgens
    •  & John C. Marioni
  • Letter |

    Ingallina et al. show that mutant p53 is protected from degradation in response to matrix stiffness in a manner dependent on RhoA geranylgeranylation and actomyosin dynamics.

    • Eleonora Ingallina
    • , Giovanni Sorrentino
    • , Rebecca Bertolio
    • , Kamil Lisek
    • , Alessandro Zannini
    • , Luca Azzolin
    • , Luisa Ulloa Severino
    • , Denis Scaini
    • , Miguel Mano
    • , Fiamma Mantovani
    • , Antonio Rosato
    • , Silvio Bicciato
    • , Stefano Piccolo
    •  & Giannino Del Sal
  • Letter |

    Glycogen metabolism controls memory T cells. Ma et al. show that the metabolic gene PCK1 promotes glycogen formation, which is used in the pentose phosphate pathway, generating glutathione that is important for counteraction of ROS and thus promotion of memory T-cell maintenance, and resulting in improved antitumour immunity.

    • Ruihua Ma
    • , Tiantian Ji
    • , Huafeng Zhang
    • , Wenqian Dong
    • , Xinfeng Chen
    • , Pingwei Xu
    • , Degao Chen
    • , Xiaoyu Liang
    • , Xiaonan Yin
    • , Yuying Liu
    • , Jingwei Ma
    • , Ke Tang
    • , Yi Zhang
    • , Yue’e Peng
    • , Jinzhi Lu
    • , Yi Zhang
    • , Xiaofeng Qin
    • , Xuetao Cao
    • , Yonghong Wan
    •  & Bo Huang
  • Letter |

    Cdk1-mediated phosphorylation of threonine and serine residues on cell cycle regulators needs to be removed after mitosis. Hein et al. show that the known preference of the PP2A–B55 phosphatase for threonine provides temporal regulation of mitotic exit.

    • Jamin B. Hein
    • , Emil P. T. Hertz
    • , Dimitriya H. Garvanska
    • , Thomas Kruse
    •  & Jakob Nilsson
  • Letter |

    Rondinelli et al. show that EZH2-mediated H3K27me3 at stalled replication forks recruits MUS81 nuclease to facilitate fork degradation. Loss of EZH2 contributes to PARPi resistance in BRCA2-deficient tumours.

    • Beatrice Rondinelli
    • , Ewa Gogola
    • , Hatice Yücel
    • , Alexandra A. Duarte
    • , Marieke van de Ven
    • , Roxanne van der Sluijs
    • , Panagiotis A. Konstantinopoulos
    • , Jos Jonkers
    • , Raphaël Ceccaldi
    • , Sven Rottenberg
    •  & Alan D. D’Andrea
  • Letter |

    Lin et al. find that stress-induced p38 MAPK activation leads to cytoplasmic relocation of the Hippo pathway nuclear transcription factor TEAD. TEAD relocation causes inhibition of YAP activity and suppresses YAP-driven cancer cell growth.

    • Kimberly C. Lin
    • , Toshiro Moroishi
    • , Zhipeng Meng
    • , Han-Sol Jeong
    • , Steven W. Plouffe
    • , Yoshitaka Sekido
    • , Jiahuai Han
    • , Hyun Woo Park
    •  & Kun-Liang Guan
  • Letter |

    Chen et al. generate lung bud organoids from human pluripotent stem cells that recapitulate early lung development, such as branching airway formation and early alveolar structures, which could potentially be used to model lung disease.

    • Ya-Wen Chen
    • , Sarah Xuelian Huang
    • , Ana Luisa Rodrigues Toste de Carvalho
    • , Siu-Hong Ho
    • , Mohammad Naimul Islam
    • , Stefano Volpi
    • , Luigi D. Notarangelo
    • , Michael Ciancanelli
    • , Jean-Laurent Casanova
    • , Jahar Bhattacharya
    • , Alice F. Liang
    • , Laura M. Palermo
    • , Matteo Porotto
    • , Anne Moscona
    •  & Hans-Willem Snoeck
  • Letter |

    Through imaging and theoretical modelling, Kimura et al. discover that endoplasmic reticulum flow determines microtubule alignment to promote cytoplasmic streaming of yolk granules in Caenorhabditis elegans zygotes.

    • Kenji Kimura
    • , Alexandre Mamane
    • , Tohru Sasaki
    • , Kohta Sato
    • , Jun Takagi
    • , Ritsuya Niwayama
    • , Lars Hufnagel
    • , Yuta Shimamoto
    • , Jean-François Joanny
    • , Seiichi Uchida
    •  & Akatsuki Kimura
  • Letter |

    Acetylation of α-tubulin on lysine 40 is associated with microtubule stability. In vitro experiments by Portran et al. show that tubulin acetylation reduces lateral interactions, increasing microtubule flexibility and resistance to mechanical stress.

    • Didier Portran
    • , Laura Schaedel
    • , Zhenjie Xu
    • , Manuel Théry
    •  & Maxence V. Nachury
  • Letter |

    Using structured illumination microscopy, Beach et al. and Hu et al. visualize the assembly of myosin II filaments in cells, describing a filament-partitioning mechanism, and long-range self-organization of filaments, respectively.

    • Shiqiong Hu
    • , Kinjal Dasbiswas
    • , Zhenhuan Guo
    • , Yee-Han Tee
    • , Visalatchi Thiagarajan
    • , Pascal Hersen
    • , Teng-Leong Chew
    • , Samuel A. Safran
    • , Ronen Zaidel-Bar
    •  & Alexander D. Bershadsky
  • Letter |

    Resnik-Docampo et al. demonstrate that depletion of the tricellular junction protein Gliotactin in young flies leads to hallmarks of ageing, including an increase in intestinal stem cell proliferation and a block in terminal differentiation.

    • Martin Resnik-Docampo
    • , Christopher L. Koehler
    • , Rebecca I. Clark
    • , Joseph M. Schinaman
    • , Vivien Sauer
    • , Daniel M. Wong
    • , Sophia Lewis
    • , Cecilia D’Alterio
    • , David W. Walker
    •  & D. Leanne Jones
  • Letter |

    Daley and colleagues report that MAPK signalling controls pluripotency in embryonic stem cells and during somatic cell reprogramming by enhancing the stability and effects of LIN28 on direct mRNA targets through its phosphorylation by ERK.

    • Kaloyan M. Tsanov
    • , Daniel S. Pearson
    • , Zhaoting Wu
    • , Areum Han
    • , Robinson Triboulet
    • , Marc T. Seligson
    • , John T. Powers
    • , Jihan K. Osborne
    • , Susan Kane
    • , Steven P. Gygi
    • , Richard I. Gregory
    •  & George Q. Daley
  • Letter |

    Ly et al. establish a method to selectively inactivate the centromere of the Y chromosome to follow chromosome shattering and micronuclei formation through several cell cycles, and suggest re-ligation of chromosome fragments is dependent on non-homologous end joining.

    • Peter Ly
    • , Levi S. Teitz
    • , Dong H. Kim
    • , Ofer Shoshani
    • , Helen Skaletsky
    • , Daniele Fachinetti
    • , David C. Page
    •  & Don W. Cleveland
  • Letter |

    Hansen et al. find that SCAI (suppressor of cancer cell invasion) is a 53BP1-binding protein that acts to repair in heterochromatin and to facilitate meiotic recombination in germ cells.

    • Rebecca Kring Hansen
    • , Andreas Mund
    • , Sara Lund Poulsen
    • , Maria Sandoval
    • , Karolin Klement
    • , Katerina Tsouroula
    • , Maxim A. X. Tollenaere
    • , Markus Räschle
    • , Rebeca Soria
    • , Stefan Offermanns
    • , Thomas Worzfeld
    • , Robert Grosse
    • , Dominique T. Brandt
    • , Björn Rozell
    • , Matthias Mann
    • , Francesca Cole
    • , Evi Soutoglou
    • , Aaron A. Goodarzi
    • , Jeremy A. Daniel
    • , Niels Mailand
    •  & Simon Bekker-Jensen
  • Letter |

    By modulating the presence of lamellipodia and filopodia, Sixt and colleagues determine that migrating dendritic cells rely on these protrusions for directed migration in complex environments, whereas locomotion per se is not driven by lamellipodia.

    • Alexander Leithner
    • , Alexander Eichner
    • , Jan Müller
    • , Anne Reversat
    • , Markus Brown
    • , Jan Schwarz
    • , Jack Merrin
    • , David J. J. de Gorter
    • , Florian Schur
    • , Jonathan Bayerl
    • , Ingrid de Vries
    • , Stefan Wieser
    • , Robert Hauschild
    • , Frank P. L. Lai
    • , Markus Moser
    • , Dontscho Kerjaschki
    • , Klemens Rottner
    • , J. Victor Small
    • , Theresia E. B. Stradal
    •  & Michael Sixt
  • Letter |

    Muthuswamy et al. report that in macrophages SCRIB interacts with the NADPH oxidase complex to promote the production of reactive oxygen species needed to kill bacteria. Conversely, loss of SCRIB promotes M1 macrophage polarization and inflammation.

    • Weiyue Zheng
    • , Masataka Umitsu
    • , Ishaan Jagan
    • , Charles W. Tran
    • , Noboru Ishiyama
    • , Michael BeGora
    • , Kiyomi Araki
    • , Pamela S. Ohashi
    • , Mitsuhiko Ikura
    •  & Senthil K. Muthuswamy
  • Letter |

    Guesdon et al.  characterize the microtubule-end-binding region of EB1 using cryo-electron tomography, providing insights into the mechanism of this interaction and the architectural changes in the GTP-cap region during microtubule growth.

    • Audrey Guesdon
    • , Franck Bazile
    • , Rubén M. Buey
    • , Renu Mohan
    • , Solange Monier
    • , Ruddi Rodríguez García
    • , Morgane Angevin
    • , Claire Heichette
    • , Ralph Wieneke
    • , Robert Tampé
    • , Laurence Duchesne
    • , Anna Akhmanova
    • , Michel O. Steinmetz
    •  & Denis Chrétien
  • Letter |

    By engineering mice to express a version of the Pten tumour suppressor that lacks the three C-terminal amino acids, van Deursen and colleagues reveal a role for the protein in the generation of symmetric spindles through recruitment of Eg5 to centrosomes.

    • Janine H. van Ree
    • , Hyun-Ja Nam
    • , Karthik B. Jeganathan
    • , Arun Kanakkanthara
    •  & Jan M. van Deursen
  • Letter |

    The imaging of individually labelled sister chromatids allows Nagasaka et al. to conclude that mitotic sister chromatin resolution begins in prophase and depends on the activity of topoisomerase II and condensin II, but not on cohesin dissociation.

    • Kota Nagasaka
    • , M. Julius Hossain
    • , M. Julia Roberti
    • , Jan Ellenberg
    •  & Toru Hirota
  • Letter |

    Vincent and colleagues show in Drosophila wing imaginal discs that the signalling molecule Wingless is synthesized and secreted at the apical surface, and is re-internalized to be transcytosed basally, where its signalling occurs.

    • Yasuo Yamazaki
    • , Lucy Palmer
    • , Cyrille Alexandre
    • , Satoshi Kakugawa
    • , Karen Beckett
    • , Isabelle Gaugue
    • , Ruth H. Palmer
    •  & Jean-Paul Vincent
  • Letter |

    Kornfeld and colleagues identify miRNAs that are dysregulated in brown adipose tissue in mouse models of obesity and ageing, and show that miR-328 targets Bace1 to promote brown adipogenesis.

    • Matteo Oliverio
    • , Elena Schmidt
    • , Jan Mauer
    • , Catherina Baitzel
    • , Nils Hansmeier
    • , Sajjad Khani
    • , Sandra Konieczka
    • , Marta Pradas-Juni
    • , Susanne Brodesser
    • , Trieu-My Van
    • , Deniz Bartsch
    • , Hella S. Brönneke
    • , Markus Heine
    • , Hans Hilpert
    • , Emilio Tarcitano
    • , George A. Garinis
    • , Peter Frommolt
    • , Joerg Heeren
    • , Marcelo A. Mori
    • , Jens C. Brüning
    •  & Jan-Wilhelm Kornfeld