Aline Wuidart, Université Libre de Bruxelles

Single-cell multi-omics sequencing of human early embryos

Latest Research

  • Resource |

    Using scCOOL-seq, Li et al. simultaneously characterize the DNA methylation and chromatin accessibility of the same cell during human preimplantation development.

    • Lin Li
    • , Fan Guo
    • , Yun Gao
    • , Yixin Ren
    • , Peng Yuan
    • , Liying Yan
    • , Rong Li
    • , Ying Lian
    • , Jingyun Li
    • , Boqiang Hu
    • , Junpeng Gao
    • , Lu Wen
    • , Fuchou Tang
    •  & Jie Qiao
  • Article |

    Sacristan et al. show that the dynein adaptor Spindly facilitates oligomerisation of the RZZ complex to expand the kinetochore, after which Spindly-associated dynein compacts the kinetochore to allow for faithful chromosome segregation.

    • Carlos Sacristan
    • , Misbha Ud Din Ahmad
    • , Jenny Keller
    • , Job Fermie
    • , Vincent Groenewold
    • , Eelco Tromer
    • , Alexander Fish
    • , Roberto Melero
    • , José María Carazo
    • , Judith Klumperman
    • , Andrea Musacchio
    • , Anastassis Perrakis
    •  & Geert JPL Kops
  • Resource |

    Using a multi-tier scRNA-seq and CRISP-seq approach, Giladi et al. define a transcriptional signature for the naive haematopoietic stem cell state, and follow progenitor plasticity and fate commitment under the influence of cytokines and growth factors.

    • Amir Giladi
    • , Franziska Paul
    • , Yoni Herzog
    • , Yaniv Lubling
    • , Assaf Weiner
    • , Ido Yofe
    • , Diego Jaitin
    • , Nina Cabezas-Wallscheid
    • , Regine Dress
    • , Florent Ginhoux
    • , Andreas Trumpp
    • , Amos Tanay
    •  & Ido Amit
  • Article |

    Signalling by the energy sensor kinase AMPK is generally tumour suppressive, but Chhipa et al. show that AMPK is upregulated in glioblastoma, where it phosphorylates CREB1 to enhance HIF1α and GABPA transcription and to support tumour bioenergetics.

    • Rishi Raj Chhipa
    • , Qiang Fan
    • , Jane Anderson
    • , Ranjithmenon Muraleedharan
    • , Yan Huang
    • , Georgianne Ciraolo
    • , Xiaoting Chen
    • , Ronald Waclaw
    • , Lionel M. Chow
    • , Zaza Khuchua
    • , Matthew Kofron
    • , Matthew T. Weirauch
    • , Ady Kendler
    • , Christopher McPherson
    • , Nancy Ratner
    • , Ichiro Nakano
    • , Nupur Dasgupta
    • , Kakajan Komurov
    •  & Biplab Dasgupta
  • Resource |

    Knapp et al. analyse the heterogeneous molecular profiles and functions of CD49f human cord blood haematopoietic stem cells and report that a subset with CD33 expression has improved regenerative activity.

    • David J. H. F. Knapp
    • , Colin A. Hammond
    • , Tony Hui
    • , Marijn T. J. van Loenhout
    • , Fangwu Wang
    • , Nima Aghaeepour
    • , Paul H. Miller
    • , Michelle Moksa
    • , Gabrielle M. Rabu
    • , Philip A. Beer
    • , Davide Pellacani
    • , R. Keith Humphries
    • , Carl Hansen
    • , Martin Hirst
    •  & Connie J. Eaves
  • Letter |

    Monitoring growing epithelial cells through the cell cycle, Uroz et al. find that cell–cell tension and cell–matrix traction forces differ across the cell cycle and affect cell cycle duration, the G1–S transition and mitotic rounding.

    • Marina Uroz
    • , Sabrina Wistorf
    • , Xavier Serra-Picamal
    • , Vito Conte
    • , Marta Sales-Pardo
    • , Pere Roca-Cusachs
    • , Roger Guimerà
    •  & Xavier Trepat

News & Comment

  • News & Views |

    AMPK is a key metabolic sensor promoting cellular energy homeostasis under low-nutrient conditions and other stresses. However, its role in cancer is context-dependent and not fully understood. A study now shows that glioma stem cells co-opt an AMPK-dependent pathway to rewire metabolism, promoting tumour growth.

    • Nektaria Maria Leli
    •  & Constantinos Koumenis
  • News & Views |

    The transition from a fertilized egg to a pluripotent and transcriptionally independent embryo requires multi-layered chromatin regulation. A study now provides simultaneous profiling of chromatin accessibility and DNA methylation in human preimplantation embryos at single-cell resolution.

    • Raquel Pérez-Palacios
    •  & Deborah Bourc’his
  • News & Views |

    Multiple clones of cancer cells co-exist within a tumour, and yet it is not clear when these subclones arise and how they contribute to tumour progression. A multicolour clonal tracing study now shows that benign skin tumours are mostly monoclonal while the more advanced lesions are composed of multiple intermixed subclones.

    • Michalina Janiszewska
    •  & Kornelia Polyak
  • News & Views |

    Phase separation can build assemblies and regulate biological function. Two articles link specific forms of protein and RNA degradation to phase separation. The polyubiquitin shuttle factor UBQLN2 localizes to stress granules where it may extract ubiquitinated proteins, and the miRISC complex functions through phase separation.

    • Tanja Mittag
    •  & Nicolas L. Fawzi
  • News & Views |

    The establishment of the two distinct lineages that form the branched epithelial ductal tree of the mammary gland is a complex and essential developmental process. Two independent studies now describe the switch from multipotency to unipotency as an embryonic process and outline mechanisms of early lineage restriction.

    • Philip Bland
    •  & Beatrice A. Howard

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