Volume 31

  • No. 12 December 2013

    Artistic rendition of hyperbranched amplicons created by multiple-displacement amplification. Gole et al. uniformly amplify the DNA of single cells in nanoliter wells, enabling copynumber variants to be detected in primary human neurons (p 1126). Credit: Kun Zhang

  • No. 11 November 2013

    Volumetric time series of false-colored GFP-histones in a live nematode embryo from the four-cell stage up to hatching. Wu et al. present diSPIM, a four-dimensional imaging method with isotropic resolution and minimal photobleaching (p 1032). Credit: Marina Spence, based on images from Yicong Wu and Hari Shroff.

  • No. 10 October 2013

    Close-up of suckers and teeth rings on the tentacles of a Humboldt squid (Dosidicus gigas). Guerette et al. use RNA-seq to rapidly identify proteins that synthesize or constitute biomaterials, including promising silklike proteins from squid sucker ring teeth (p 908). Credit: Louise Murray/ Visuals Unlimited/Corbis

  • No. 9 September 2013

    Heat maps of the specificity of DNA cleavage by RNA-guided Cas9 endonuclease. Four groups use a variety of methods in vitro and in cells to assess the specificity of genome editing with CRISPR-Cas systems (pp 822, 827, 833, 839). Credit: Marina Spence, based on images provided by David Liu, Prashant Mali and Feng Zhang.

  • No. 7 July 2013

    Confocal image of liver section from mice injected with lipid nanoparticles carrying siRNA (red). Gilleron et al. (p 638) and Sahay et al. (p 653) dissect molecular mechanisms of siRNA delivery to guide the development of RNAi therapeutics. Credit: Jerome Gilleron, Anja Zeigerer and Marino Zerial.

  • No. 6 June 2013

    Global mapping of cancer gene expression changes to the human metabolic network. Hu et al. find substantial heterogeneity in how cellular metabolism is reprogrammed across 22 cancers (p 522). Credit: Jie Hu and Dennis Vitkup using the iPath tool.

  • No. 5 May 2013

    A bacterial cell endowed with logic and memory. Siuti et al. open new doors for cellular engineering by synthesizing genetic circuits whose function depends on past and present inputs (p 448). Credit: Liang Zong and Yan Liang (l2xy2.com).

  • No. 4 April 2013

    Bacteria 1 Credit: Art by Angelina Vick www.angelinavick.com

  • No. 3 March 2013

    Artistic rendition of DNA cleavage directed by the Cas9 endonuclease complexed with a synthetic single guide RNA. Three groups demonstrate site-specific genome modification in zebrafish embryos (p 227), human cells (p 230) and bacteria (p 233). Credit: Stephen Dixon

  • No. 2 February 2013

    Cherry and currant tomatoes of varying ripeness. Zhong et al. use whole-genome cytosine methylation and transcription factor binding analyses to show that epigenome dynamics influence the developmental transition to ripening (p 154). Credit: Ryan McQuinn

  • No. 1 January 2013

    A fractal-like hierarchy of network clusters extracted from the interaction networks of budding yeast. Dutkowski et al. use cellular components and processes embedded in molecular networks to derive a gene ontology (p 38). Credit: Janusz Dutkowski