Volume 28

  • No. 12 December 2010

    Oligonucleotides generated on a microarray provide an abundant source of raw material for assembling long synthetic DNA molecules. Kosuri et al. and Matzas et al. reduce the error rate and improve the scalability of approaches that use microarray oligonucleotides to synthesize genes and other DNA elements (pp 1291 and 1295). Credit: Marina Corral, based on idea courtesy of Sriram Kosuri and Mark Matzas.

  • No. 11 November 2010

    Therapeutic IgG antibodies bind only two antigen molecules during their lifetime. Igawa et al. engineer antibody recycling through pH-dependent binding, which allows the antigen to be released in the endosome for lysosomal degradation while the antibody is returned to the plasma for additional rounds of antigen binding (p 1203). Credit: JOLLYBOY and Sakura Motion Picture Co., Ltd.

  • No. 10 October 2010

    The painting Histone Subunit Exchange portrays the dynamic nature of nucleosome structure. This issue focuses on the role of epigenetics in health and disease and discusses the therapeutic prospects of targeting the epigenetic machinery. Credit: David Sweatt.



  • No. 9 September 2010

    Seeds of the castor bean plant, Ricinus communis. Chan et al. report a draft genome of this oilseed crop, the first for a member of the Euphorbiaceae (p 951). Credit: Pablo Rabinowicz

  • No. 8 August 2010

    A computer-generated representation of HIV on the surface of a T lymphocyte. Holt et al. block the entry of HIV into blood cells by using zinc finger nucleases to knock out CCR5 in hematopoietic stem cells (p 839). Credit: ANIMATE4.com/SciencePhotoLibrary

  • No. 7 July 2010

    Crystal structure of the WD40 domain of the yeast SCFCdc4 E3 ubiquitin ligase showing the binding of an allosteric inhibitor and the subsequent displacement of the substrate. Orlicky et al. and Aghajan et al. present the first inhibitors of cullin-RING ubiquitin ligases, the largest class of enzyme that confers specificity to the ubiquitin-proteasome system. (pp 733 and 738).

  • No. 6 June 2010

    Artist’s impression of digital ELISA. Rissin et al. determine protein abundance by capturing beads bearing single analyte molecules in arrayed femtoliter-volume reaction chambers (p 595). Credit: Ken Eward © BioGrafx.

  • No. 5 May 2010

    Kidney glomeruli. The progress of the Nephrotoxicity Working Group of the Predictive Safety Testing Consortium towards validating markers of kidney damage is presented on p 430. Artwork by Lewis Long.

  • No. 4 April 2010

    Light micrograph of cultured human breast cancer cells. Weinberg and colleagues show that targeting a pro-metastatic microRNA with antagomirs prevents the formation of new metastases in a mouse model of breast cancer (p 341). Credit: Cecil H. Fox/Science Photo Library

  • No. 1 January 2010

    Structures of phosphodiesterase 4 in open and closed conformations and several allosteric modulators. Burgin, Gurney and colleagues solve crystal structures of phosphodiesterase 4 bound to ligands and design allosteric modulators with pro-cognitive effects. (p 63)