Volume 23

  • No. 12 December 2005

    Image of a mouse embryo undergoing gastrulation, in which the three embryonic germ layers —ectoderm, mesoderm and endoderm — are formed. Two papers in this issue describe the differentiation of embryonic stem cells to definitive endoderm (p 1534; p 1542). Image courtesy Olivia Kelly.

  • No. 11 November 2005

    Two male larvae of the malaria vector mosquito Anopheles stephensi showing expression of a green fluorescent protein in their gonads. Differentiating males from females is a crucial aspect of the sterile insect technique (SIT) for mosquito control. Catteruccia et al. describe a genetic sexing technique to identify sterile male mosquitoes for their potential use in SIT programs for controlling malaria, p 1414.

  • No. 10 October 2005

    An aggregate of human pancreatic β-cells. The red dye indicates cytoplasmic insulin, and the green dye indicates nuclear Pdx-1. Narushima et al. report a reversible immortalization strategy for generating large numbers of functional human β-cells, (p 1274).

  • No. 9 September 2005

    This issue focuses on antibody engineering and manufacturing. The stylized image of antibodies is by Jacque Deshaies and is entitled "Deshaies 99—Antibody #1 to #4" (original 4: 28 x 36 cm, acrylic on paper) http://www.JacquesDeshaies.com

  • No. 8 August 2005

    Pathways involved in the osmotic shock response in yeast cells. A comprehensive model of this process that incorporates molecular and biophysical components is reported by Klipp et al.(p. 975). Photo by SciMAT/Photo Researchers, Inc.

  • No. 7 July 2005

    'The Creation of Organs' depicts the potential to create organs and tissues from stem cells. This issue includes a focus on cell therapies for regenerative medicine. Painting by Hunter O'Reilly, Loyola University, Chicago

  • No. 6 June 2005

    Nicotiana benthamiana plants expressing green fluorescent protein (GFP) following transfection with an 'mRNA-like', tobacco mosaic virus-derived expression vector containing the GFP gene (picture taken under UV light). Marillonnet et al. describe the application of this expression vector to recombinant protein production in plants (p 718).

  • No. 5 May 2005

    An unstained brain section from an APP23 transgenic mouse, an animal model of Alzheimer disease. Amyloid plaques are visible as small dark dots, most of them in the neocortex. Hintersteiner et al. describe a new probe for imaging amyloid plaques in living mice (p 577).

  • No. 4 April 2005

    Genetically engineered cows expressing an antibacterial enzyme in their milk resist mastitis. (Wall et al., p 445)

  • No. 3 March 2005

    Transverse section of a hind leg muscle of a 6-month-old mouse showing GFP gene expression following delivery by an adeno-associated virus 8 vector (tibia and fibula bones and muscle cell nuclei are stained red). A comparative study shows that this vector enables efficient system-wide gene delivery to muscle tissues. (Wang et al.p 321).

  • No. 2 February 2005

    Representation of siRNA and shRNA processing by the RNAse III-family nuclease Dicer. Two papers describe the optimization of RNAi molecules for more efficient gene silencing. (see Kim et al.p. 222 and Siolas et al. p. 227). Graphic by Doug Huff © Arkitek.

  • No. 1 January 2005

    A color-enhanced scanning electron micrograph of red blood cells. Fully mature red blood cells have been generated in culture from hematopoietic stem cells (Giarratana et al., p 69). Credit: Biophoto Associates/Photo Researchers, Inc.