3D image of the mitochondrial compartment of a live yeast cell obtained through volume rendering of a superresolution 3D data stack recorded with a 4Pi microscope. Courtesy of H. Gugel, R. Storz (Leica Microsystems Heidelberg GmbH, Mannheim, Germany) and J. Bewersdorf, S. Jakobs, J. Engelhardt, S.W. Hell (MPI for Biophysical Chemistry, Göttingen, Germany). The 3D image is superimposed on a colored high resolution scanning electron micrograph of mitochondria and rough endoplasmic reticulum. © PhotoResearchers. Artwork rendered by Erin Boyle.