Abstract
In the following review, I present an assessment of the realities and possibilities of effecting gene transfer to cereal crops. I discuss why Agrobacterium has been unsuccessful with cereals, what alternatives have been tested, the extent to which they have yielded transgenic plants, and their potential agronomic utility. The discussion, necessarily subjective, is framed within a rigid definition of what constitutes proof of gene integration, and the biological factors affecting transformation competence.
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References
Gasser, C.S. and Fraley, R.T. 1989. Genetically engineering plants for crop improvement. Science 244: 1293–1299.
Kahl, G. 1982. Molecular biology of wound healing: the conditioning phenomenon. In: Molecular Biology of Plant Tumors. G. Kahl, J. Schell (Eds.), p. 211–267, Academic Press, NY.
Vasil, I.K. 1987. Developing cell and tissue culture systems for the improvement of cereal and grass crops. J. Plant Physiol 128: 193–218.
Hooykaas, P.J.J. 1989. Transformation of plant cells via Agrobacterium. Plant Mol. Biol. 13: 327–336.
Klee, H.J. and Rogers, S.G. 1989. Plant gene vectors and transformation: Plant transformation systems based on the use of Agrobacterium tumefaciens. p. 2–25. In: Cell Culture and Somatic Cell Genetics. Vol. 6. Molecular Biology of Plant Nuclear Genes. Schell, J. and Vasil, I. K. (Eds.), Academic Press, San Diego.
Binns, A.N. 1990. Agrobacterium-mediated gene delivery and the biology of host range limitations. Physiol. Plant. 78(1), In press.
Tepfer, D. 1990. Genetic transformation using Agrobacterium rhizogenes. Physiol. Plant. 78(1), In press.
Graves, A.C.F. and Goldmann, S.L. 1986. The transformation of Zea mays seedlings via the Agrobacterium tumefaciens. Plant Mol. Biol. 7: 43–50.
Schäfer, W., Gorz, A. and Kahl, G. 1987. T-DNA integration and expression in a monocot crop plant after induction of Agrobacterium. Nature 327: 529–532.
King, P.J., Potrykus, I. and Thomas, E. 1978. In vitro genetics of cereals: problems and perspectives. Physiol. Vég. 16: 381–399.
Grimsley, N.H., 1990. Agroinfection. Physiol. Plant. 78(1), In press.
Gardner, R., Chonoles, K. and Owens, R. 1986. Potato spindle tuber viroid infections mediated by the Ti-plasmid of Agrobacterium tumefaciens. Plant Mol. Biol. 6: 221–228.
Grimsley, N.H., Hohn, T., Davies, J.W., Hohn, B. 1987. Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325: 177–179.
Fütterer, J., Bonneville, J.M., Hohn, T. 1990. Cauliflower mosaic virus as a gene expression vector for plants. Physiol. Plant. 78(1), In press.
Brisson, N., Paszkowski, J., Penswick, J.R., Gronenborn, B., Potrykus, I. and Hohn, T. 1984. Expression of a bacterial gene in plants by using a viral vector. Nature 310: 511–514.
Ahlquist, P. and Pacha, R.F. 1990. Gene amplification and expression by RNA viruses and potential for further application to plant gene transfer. Physiol. Plant. 78(1), In press.
Ahlquist, P., French, R., Bujarski, J.J. 1987. Molecular studies of brome mosaic virus using infectious transcripts from cloned cDNA. Adv. Virus Res. 32: 214–242.
Töpfer, R., Gronenborn, B., Schaefer, S., Schell, J. and Steinbiss, H.H. 1990. Expression of engineered wheat dwarf virus in seed derived embryos. Physiol. Plant. 78(1), In press.
Töpfer, R., Gronenborn, B., Schell, J. and Steinbiss, H.H. 1989. Uptake and transient expression of chimeric genes in seed-derived embryos. The Plant Cell 1: 133–139.
Ledoux, L. and Huart, R. 1969. Fate of exogenous bacterial desoxyri-bonucleic acids in barley seedlings. J. Mol. Biol. 43: 243–262.
Ledoux, L., Huart, R. and Jacobs, M. 1974. DNA-mediated genetic correction of thiaminless Arabidopsis thaliana. Nature 249: 17–21.
Luo, Z. and Wu, R. 1988. A simple method for transformation of rice via the pollen-tube pathway. Plant Mol. Biol. Reporter 6: 165–174.
Caboche, M. 1990. Liposome-mediated transfer of nucleic acids in plant protoplasts. Physiol. Plant. 78(1), In press.
Gad, A.E., Rosenberg, N. and Altmann, A. 1990. Liposome-mediated gene delivery into plant cells. Physiol. Plant. 78(1), In press.
Ahokas, H. 1987. Transfection by DNA-associated liposomes evidenced at pea pollination. Hereditas 106: 129–138.
Lucas, W.J., Lansing, A., deWet, J.R. and Walbot, V. 1990. Introduction of foreign DNA into walled plant cells via liposomes injected into the vacuole: a preliminary study. Physiol. Plant. 78(1), In press.
Paszkowski, J., Saul, M.W. and Potrykus, I. 1989. Plant gene vectors and genetic transformation: DNA-mediated direct gene transfer to plants. p. 52–68. In: Cell Culture and Somatic Cell Genetics of Plants. Vol. 6. Molecular Biology of Plant Nuclear Genes. Schell, J. and Vasil, I. K. (Eds.). Academic Press, San Diego.
Davey, M.R., Rech, E.L. and Mulligan, B.J. 1989. Direct DNA transfer to plant cells. Plant Mol. Biol. 13: 273–285.
Potrykus, I., Shillito, R.D., Saul, M.W. and Paszkowski, J. 1985. Direct gene transfer. State of the art and future potential. Plant Mol. Biol. Rep. 3: 117–128.
Paszkowski, J., Baur, M., Bogucki, A. and Potrykus, I. 1988. Gene targeting in plants. EMBO J. 7: 4021–4027.
Shimamoto, K., Terada, R., Izawa, T. and Fujimoto, H. 1989. Fertile rice plants regenerated from transformed protoplasts. Nature 338: 274–276.
Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D. and Detmer, J.J. 1989. Genetically transformed maize plants from protoplasts. Science 240: 204–207.
Vasil, I.K. 1988. Progress in the regeneration and genetic manipulation of cereal crops. Bio/Technology 6: 397–401.
Shillito, R.D., Carswell, G.K., Johnson, C.M., DiMiaio, S. and Harms, C.T. 1989. Regeneration of fertile plants from protoplasts of elite inbred maize. Bio/Technology 7: 581–587.
Prioli, L.M. and Söndahl, M.R. 1989. Plant regeneration and recovery of fertile plants from protoplasts of maize. Bio/Technology 7: 589–594.
Potrykus, I. 1980. The old problem of protoplast culture: cereals. p. 243–254. In: Advances in protoplast research. Ferency, L., Farkas, G.L. (Eds.). Pergamon Press, Oxford.
Weber, G., Monajembashi, S., Greulich, K.O. and Wolfrum, J. 1990. Genetic changes induced in higher plant cells by a laser micro beam. Physiol. Plant. 78(1), In press.
Ahokas, H. 1989. Transfection of germinating barley seed electrophoretically with exogenous DNA. Theor. Appl. Genet. 77: 469–472.
Sanford, J.C. 1990. Biolistic plant transformation. Physiol. Plant. 78(1), In press.
Klein, T.M., Fromm, M.E., Gradziel, T. and Sanford, J.C. 1988. Factors influencing gene delivery into Zea mays cells by high-velocity microprojectiles. Bio/Technology 6: 559–536.
McCabe, D.E., Swain, W.E., Martinell, B.J. and Christou, P. 1988. Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6: 923–926.
Christou, P., McCabe, D.E. and Swain, W.F. 1988. Stable transformation of soybean callus by DNA-coated gold particles. Plant Physiol. 87: 671–674.
Neuhaus, G., Sopangenberg, G., Mittelsten-Scheid, O. and Schweiger, H.G. 1987. Transgenic rapeseed plants obtained by microinjection of DNA into microspore-derived proembryoids. Theor. Appl. Genet. 75: 30–36.
Neuhaus, G. and Spangtenberg, G. 1990. Plant transformation by microinjection technique. Physiol. Plant. 78(1), In press.
De la Pena, A., Lörz, H. and Schell, J. 1987. Transgenic plants obtained by injecting DNA into young floral tillers. Nature 325: 274–276.
Hess, D. 1987. Pollen based techniques in genetic manipulation. Int. Rev. Cytol. 107: 169–190.
DeWet, J.M.J., Bergquist, R.R., Harlan, J.R., Brink, D.E., Cohan, C.E., Newell, C.A. and DeWet, A.E. 1985. Exogenous DNA transfer in maize (Zea mays) using DNA-treated pollen. p. 197–209. In: Experimental Manipulation of Ovule Tissue. Chapman, G. P., Mantell, S. H., Daniels, W. (Eds.). Longman, London.
Ohta, Y. 1986. High efficiency genetic transformation of maize by a mixture of pollen and exogenous DNA. Proc. Natl. Acad. Sci. USA 83: 715–719.
Alwen, A., Eller, N., Kastler, M., Benito-Moreno, R.M. and Heberle-Bors, E. 1990. Potential of in vitro pollen maturation for gene transfer. Physiol. Plant. 78(1), In press.
Fromm, M.E., Taylor, L.P. and Walbot, V. 1986. Stable transformation of maize after electroporation. Nature 319: 791–793.
Shillito, R.D., Saul, M.W., Paszkowski, J., Müller, M. and Potrykus, I. 1985. High frequency direct gene transfer to plants. Bio/Technology 3: 1099–1103.
Lindsay, K. and Jones, M.K.G. 1990. Electroporation of cells. Physiol. Plant. 78(1), In press.
Potrykus, I. 1990. Gene transfer to plants: assessment and perspectives. Physiol. Plant 78(1), In press.
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Potrykus, I. Gene Transfer to Cereals: An Assessment. Nat Biotechnol 8, 535–542 (1990). https://doi.org/10.1038/nbt0690-535
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DOI: https://doi.org/10.1038/nbt0690-535
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